Introduction Thin-layer Chromatography(TLC) was the method used to figure out and identify the drug. TLC is done on a plate that is coated with a layer of absorbent material.after the solutions have been spotted onto the plate‚ a solvent is then drawn up the plate with capillary action. Capillary action has to do with the intermolecular forces with the liquid and the surroundings solid surface Different substances ascend the plates with different rates. After the TLC was completed the retention factors
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the Rf of a known compound with the Rf of an unknown compound. Intodection. In this experiment the thin-layer chromatography the (TLC) will be used to determine the composition of various over-the-counter analgesic. We will be given two commercially prepared TLC plates with a flexible backing and a silica-gel coating with a fluorescent indicator. On the first TLC plate‚ a reference plate‚ we will spot five standard compounds . In addition‚ a standard reference mixture containing four of these
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sulfate as the phase-transfer catalyst. Monitor the progress of the reaction by thin-layer chromatography. BACKGROUND REQUIRED You should be familiar with extraction‚ evaporation‚ and thin-layer chromatography techniques. You may wish to review the TLC lab from earlier in the semester! BACKGROUND INFORMATION In organic molecules‚ carbon may exist in several different oxidation states. In typical organic compounds‚ the oxidation number for carbon may vary from –4 (a very reduced form of carbon‚
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the recrystallization was very effective in removing impurities. Conclusion: When doing this experiment I made a batch of crude benzoic acid‚ this batch was meant to be used for a titration‚ recrystallization‚ six melting points and a TLC experiment. When I started the experiment I believed that I would have enough crystals to complete all experiment with some crystals left over‚ however once the titration came about I realised that I did not have enough benzoic acid‚ therefore instead
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colored components of plant leaves. Today‚ the name is a bit misleading‚ because most forms of chromatography do not depend on color. Several types of chromatography are commonly used‚ among which are paper chromatography‚ thin-layer chromatography or TLC‚ liquid-liquid chromatography‚ gas chromatography‚ and high performance liquid chromatography or HPLC. Chromatography is so useful that some form can be found in most scientific laboratories around the world. For example‚ in forensic chemistry crime
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partitioning‚ this separation technique exploited the different polarity of the compounds in the spinach extract. Three fractions with different colors were obtained. The extract and its fractions were analyzed using thin layer chromatography (TLC). The TLC results showed that there was one compound (Rf=0.979) in the first fraction; there were three compounds (Rf1=0.839‚ Rf2= 0.691‚ Rf3=0.149) in the second fraction; there was one compound (Rf=0.017) in the third fraction. The separations of compounds
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Experiment 3: Isolation of Chlorophyll from Spinach and Chromatography Performed February 14th and 16th By Kyle Herzog Organic Chemistry 344 Section 807 Spring 2012 Objective: The purpose of the experiment is to extract pigments from spinach leaves and separate them by column and thin layer chromatography‚ determining Rf values for the pigments. Equations: Rf= Distance travelled by a given compound/Distance travelled by a solvent solution 1. Crude- 4.25/5.5= 0.773 2. Carotene-
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and Health‚ Dublin Institute of Technology‚ Cathal Brugha Street‚ Dublin 1‚ Ireland nissreen.abughannam@dit.ie Received: November 28th‚ 2010; Accepted: May 22nd‚ 2011 Vegetables belonging to the Brassicaceae family are rich in polyphenols‚ flavonoids and glucosinolates‚ and their hydrolysis products‚ which may have antibacterial‚ antioxidant and anticancer properties. In the present study‚ phenolic composition‚ antibacterial activity and antioxidant capacity of selected Brassica vegetables
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2-15-11 Purpose: The purpose of this experiment was to take spinach leaves and extract the chlorophyll and carotenoid pigments by using acetone as the solvent. The chlorophyll and carotenoid pigments were extracted by using column chromography and alumina was used as the solvent. Solvents of different polarities were used‚ starting with the least polar‚ to extract the certain components from the leaves. They were then analyzed by using thin- layer chromatography. Procedure: The first part
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Student Name: Melissa Tatum Student ID: 4593119 Date: 7 Dec 2014 Course and Section Number: SCIN131 A004 Fall 14 Lesson 4 Lab: Chromatography and Ionic versus Covalent Bonds PART 1 Begin by viewing the following Thinkwell video 15.1.3 CIA Demonstration: Chromatography After you watch the above video‚ answer the questions below in sufficient detail: (a) (3 pts.) This video discusses 3 different types of chromatography. List each one mentioned‚ and describe their differences in as much detail
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