decreasing polarity (this can be done according to structure of the individual solvents and % composition of the mixtures). Also rank the 3 compounds in order of decreasing polarity (this compound ranking can be based on structure as well as on these TLC results). Explain each of your rankings. Compounds: 9-fluorenone‚ 9-fluorenol. Fluorene Solvent: Ethyl acetate 10% in hexane‚ Ethyl acetate 25% in hexane‚ 100% hexane Polarity of each compound reacted differently to each of the solvents used
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Abstract: In this lab‚ the chemical components of Excedrin and Tylenol‚ two common‚ over-the-counter analgesic drugs‚ were determined by utilizing the technique of thin-layer chromatography‚ or TLC. The active ingredients of Excedrin were caffeine‚ acetaminophen‚ and aspirin with Rf values of 0.11‚ 0.38‚ and 0.61‚ respectively. The lone active ingredient of Tylenol was acetaminophen with an Rf value of 0.39. The elution sequence obtained in the experiment is as follows: ibuprofen (least polar)
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Dyes by TLC Pre-lab Properties: Ethyl acetate‚ ethanol‚ silica‚ azobenzene‚ azulene‚ 4-(p-nitrophenylazo) resorcinol‚ methyl red‚ bromocresol green (solubilities in water and ethanol) Purpose: To identify compounds from an unknown mixture using TLC Up to 100% of missed points can be recovered from this lab Watch the video: http://www.youtube.com/watch?v=e99nsCAsJrw (MIT) TLC plates are near the main hood DO NOT BREAK CAPILLARY TUBES Keep spots small and concentrated (video) Stop TLC before solvent
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Expt. 8.1 Thin Layer Chromatography (TLC) Analysis of Analgesics Krista Jinks CH 211L Section F 10/27/14 I. Abstract: The objective of this experiment was to analyze analgesic tablets by TLC to determine what analgesics they contain. The analgesics that could be in the tablets included ibuprofen‚ acetaminophen‚ aspirin‚ and caffeine. Through the use of TLC‚ unknown substance #62 was found to be Bayer Aspirin because it only contained aspirin. II. Introduction: 1. Briefly describe
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A- HPTLC Flavonoid profile of methanol extract M. concanensis leaves B- HPTLC Flavonoid profile of methanol extract M. concanensis flowers C- HPTLC Flavonoid profile of methanol extract M. concanensis seeds 4.2.3.1 C) HPTLC Phenol profile of M. concanensis Nimmo. HPTLC finger printing of M. concanensis was done by using selected solvent system Chloroform: Ethyl acetate: Formic acid (50%:40%:10%v/v) for leaf‚ flower and seed extracts‚ visualized under UV 254 and 366 nm showed more
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Experiment 3: The preparation of acetaminophen (paracetamol) with thin layer chromatography (TLC) to monitor the reaction. Abstract: This experiment is to demonstrate the preparation of paracetamol and its properties. Reflux and filtration of 4-aminophenol and acetic anhydride formed the crude sample. Further analysis of dry white crystals were used to give quantitative measurements and a percentage yield of 46% was obtained. The overall conclusion is that the acetic anhydride reacted with the
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factors that might affect the percentage yield‚ such as influence of the harvest place‚ harvest period‚ type of soil and climate. 4.2 Phytochemical analysis 4.2.1 Phytochemical compounds determination Saponins‚ alkaloids‚ tannins‚ flavonoids and Phenolic compounds were qualitatively analyzed. The summary of phytochemical analysis results of aqueous crude extracts of C. nardus leaves was tabulated in table 4.1. Table 4.1. Phytochemical analysis results of aqueous crude extracts of
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Thin Layer Chromatography (TLC) 5.0 Detection and Identification 7 6.4 UV-Vis Spectrophotometry 6.5 Mass Spectrometry 6.6 Nuclear Magnetic Resonance Spectrometry 6.0 Health Benefits of Daidzein 18 7.7 Exposure to Daidzein 7.8 Anti-Tumour Effect of Isoflavone 7.9 Daidzein and Cardioovascular Disease 7.0 Conclusion 21 8.0 References 22 1.0 Introduction Flavonoids are present in plants as mixtures
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Chromatography – Analyzing Analgesics by TLC and Isolation of β-Carotene by Column Chromatography Introduction/Background: Flavonoids are an important group of additives that can be defined as pure substances either natural‚ extracted from raw materials or synthetic. Chromatography is the separation of two or more compounds or ions caused by their molecular interactions with two phases – one moving and one stationary (Weldegirma 2012). Three types of chromatography are used
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ether‚ methanol‚ distilled water‚ and ethanol solvents. Evaluation of phytochemicals such as alkaloids‚ flavonoids‚ carbohydrates‚ glycosides‚ protein and amino acids‚ saponins‚ and triterpenoids revealed the presence of most of the constituent in polar extracts such as ethanol‚ methanol‚ and aqueous extracts compared with nonpolar extracts (petroleum ether and chloroform). However‚ flavonoids‚ proteins and amino acids‚ tannins‚ and phenols were found to be universally occurring in all the extracts
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