3.2 DNA Extraction The DNA will be extracted from the Nemipterus samples according to Wizard® Genomic DNA Purification Kit (Promega) protocols. The first step of is cells and nuclei will be lysed by adding 120 µl of 0.5 Molar ethylenediaminetetraacetic acid (EDTA) to 500 µL of Nuclei Lysis Solution in a eppendorf tube‚ then it will be chilled on ice until the solution turn cloudy. The second step is 0.5 cm of Nemipterus sample tissue will be minced to fine powder. The fine powder of fresh Nemipterus
Premium DNA Protein Molecular biology
Light at the End of the Tunnel When I graduated from high school I was like all the other low-life‚ lazy teenagers‚ not yet ready to be taken from my parent’s wings. I never realized the importance of furthering my education. I just knew I could “do big things” working as a Sales Associate at 7-Eleven for the rest of my life. After my first child it began to dawn on me that an education would be the only thing that would let me provide for my family in the ways I wanted to. How scary is that I
Premium 2006 albums English-language films High school
Photosynthesis and Semi-Conductor based Solar Cells Plants‚ by shear desire to survive have developed the worlds most efficient and reliable power supply by way of photosynthesis. Scientist for years have tried unsuccessfully to duplicate the process of photosynthesis. However‚ for the purpose of this paper‚ I will compare and contrast how energy and electricity is generated through photosynthesis and a semi-conductor based solar cell. In addition‚ this paper will explain how both processes
Premium Photovoltaics Entropy Temperature
Automatic Street Light [pic] Submitted By: Introduction Automatic Streetlight needs no manual operation for switching ON and OFF when there is need of light. It detects itself weather there is need for light or not. When darkness rises to a certain value then automatically streetlight is switched ON and when there is other source of light i.e. day time‚ the street light gets OFF. The sensitiveness of the street light canal so be adjusted
Premium Resistor Ohm's law Voltage divider
Ticie and Stella See were mother- and daughter-in-law‚ from different generations and backgrounds‚ but with the same yearning for a place to belong‚ a family to surround themselves with‚ and a way to make their mark on the world. Both women grew up in poverty‚ shuttled around to various relatives‚ never a place to settle down. They were a product of the place and time in which they grew up‚ yet they evolved beyond what should have been shuttered‚ sheltered slums and found beauty in a new culture
Premium Family English-language films Marriage
Ryan Getherall November 3‚ 2014 Light/Dark Paragraphs In the novel‚ A Tale of Two Cities‚ by Charles Dickens‚ the author uses light and dark to set the tone. The tone of this novel is mysterious‚ dark‚ and depressing. At the beginning of the novel‚ Dickens says “It was the season of Light‚ it was the season of Darkness‚ it was the spring of hope‚ it was the winter of despair” (7). In this quote‚ Dickens uses paradox to explain the controversy between the light and darkness throughout. When
Premium
the speed of light “So far as problems involving light are concerned‚ the electrons (in atoms) behave as though they were held by springs”.Feynman “In a microscopic but otherwise classical analysis‚ the electric field in electromagnetic radiation accelerates electrons held by springs in the atoms of a piece of glass‚ and these accelerated electrons re-radiate in all directions. The observed light is the superposition of the electric (and magnetic) fields of the incoming light and the re-radiation
Premium Wave Fundamental physics concepts Electric charge
A Light Bulb Moment What is a light bulb moment? A light bulb moment can mean a sudden inspiration‚ revelation‚ or recognition. There many moments in life that bring on an light bulb moment. I get numerous moments where the light bulb goes off and i think of something. That will work well with my originality. These ideas started just as i began to write. "Ah! Now I see" i suddenly realized that this was an immediate light bulb moment for me as I thought of what
Premium Incandescent light bulb Writing Light
Photosynthesis and Cellular Respiration Nina R. McCray May 5‚ 2015 Biology Lab (SCIE207-1502-A-07) LaDonna McCowan-Ferrier Photosynthesis and Cellular Respiration Lab Report Introduction This is an investigation of the effect of the intensity of light (photosynthesis) have on the rate of cellular respiration (measured as the number of oxygen bubbles). We will determine whether or not it is possible to examine the relationship between photosynthesis and cellular respiration under controlled
Premium Oxygen Carbon dioxide Cellular respiration
Lights‚ Camera‚ History Book Review Lights‚ Camera‚ History takes the reader through history within the film. A large problem this book faces is the depiction of history within films and the challenges directors face. This book provides an enthusiastic argument in the defense of directors saying the purpose is to make money not be historians. It is important to tell students do not accept what you see as fact. In the chapter “In Praise of the Biopic‚” the author talks about: “Reds‚ They Died with
Premium Film Racism Film director