The main goal of this experiment was to successfully clone human insulin cDNA into an expression vector. The gel electrophoresis results confirm that the inserts were successfully cloned into the expression vector based on the identical fragment position of each cut candidate in the gel. The fragment sizes of the both cut candidates produced on the gel when compared to the DNA ladder were larger‚ at roughly about 6000 base pairs and 6800 base pairs‚ than the predicted size of 2315 and 3119 base pairs
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Secondary excitotoxicity is thought to be more relevant to chronic neurodegenerative disorders than the classical acute form. In this pathway increased glutamate levels are not required as disturbances with the neuronal resting membrane potential cause glutamate receptors to be over-activated by physiological levels of glutamate. In a neuron which is affected by another pathological process and shows reduced ATP production‚ Na+ dysfunction or impaired glucose metabolism and as a result has an abnormal
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The movie Juno is fiction and came out in 2007. It is about Juno MacGuff a 16 year old high schooler who gets pregnant after her first time having intercourse with Paulie Bleeker‚ who is also in high school. Although 9 years old‚ this movie is still extremely relevant in today’s society in regards to teen pregnancy. It tackles all the different options one can take and how emotionally‚ physically‚ and mentally challenge this decision is‚ especially when you’re a teen. Psychologist David Elkind theorized
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The nonmutated form of DMPK functions in the production of protein kinase serine; this enzyme plays a role in intracellular communication and in the regulation of myosin phosphate. When DMPK is mutated‚ the proteins CUG-BP1‚ MBNL1 and INSR are displaced causing the splicing of troponin pre-mRNA which causes malfunctions in the cardiac conduction system‚ dominance of a chloride channel that causes myotonia‚ dominance of an insulin receptor that affects the sufficiency of insulin‚ and dominance of
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Introduction: The pharmacokinetics of a drug describes the journey of a drug through the body i.e. its absorption‚ bioavailability‚ distribution‚ metabolism and excretion. Opioids are substances (endogenous or synthetic)‚ which produce ‘opiate-like effects’. Opiates are alkaloids derived from the Papaver somniferum poppy e.g. Morphine‚ Codeine (a morphine-like agonist) and Methadone (a synthetic analogue of morphine) and are primarily used as analgesics.[1] Discussion: Morphine has a 4-ring structure
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How to Write a Synthesis Essay Synthesis Writing: to combine the ideas of more than one source with your own. Key Features of a Synthesis Report information from the sources using different phrases and sentences; Organize so that readers can immediately see where information from the sources overlap; Make sense of the sources and help the reader understand them in greater depth. Preparing to write your Synthesis Essay You should locate two or more sources for synthesizing.
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Persuasive Essay Topics are strong essays designed to convince the reader that the point demonstrated is the correct opinion that others should share. Topics are typically controversial and often involve a combination of research and appealing writing. When developing a good persuasive essay topic‚ it is essential to avoid exaggeration and fallacies in reading. The paper must demonstrate critical thinking skills; however‚ it may be written less formally than research essays or literary essays. A
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KANCHAN AHUJA Lily Villa‚ 12th Road‚ Chembur‚ Mumbai – 400 071 Phone Nos: (R) 022- 25217372 (M) 98207 10041 Email : kanchanahuja@rediffmail.com Currently persuing Masters in management from K J Somaiya Institute of management Batch – 2008-2011 Objective: To secure a challenging and rewarding position within a reputable company offering personal development opportunities based on performance. Work Experience: HSBC Bank : Designation:
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Have Faith “I’d like to repeat the advice that I gave you before‚ in that I think you really should make a radical change in your lifestyle and begin to boldly do things which you may previously never have thought of doing‚ or been too hesitant to attempt. So many people live within unhappy circumstances and yet will not take the initiative to change their situation because they are conditioned to a life of security‚ conformity‚ and conservatism‚ all of which may appear to give one peace of mind
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Becca Benfield Scraping Bottom Summary: This article begins with a story about a man in Alberta‚ Canada. When he was young he would spend ample time with his grandfather. One day in 1963‚ when he was 7 years old‚ they arrived back at the house only to see that his log cabin had been destroyed without any warning or notice. This was the beginning of the Alberta oil sands. After oil was discovered on his land‚ the whole valley becomes covered with open pit mines. In order to create
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