use of pGlo plasmids. II Materials and Procedures The first procedure of the lab was to obtain two microtubes and label one +pGlo and the other –pGlo with a marker. Next‚ the tubes were placed in the microtube rack and 250 μl of CaCl2 was transferred into each tube using a sterile pipette. After placing the microtubes in ice‚ a sterile inoculation loop was used to pick up a single colony of bacteria from the E. coli starter plate. Next‚ the +pGlo microtube was removed from the ice and‚ to ensure
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Analysis of Soda Ash and Volumetric Analysis of a Carbonate-Bicarbonate Mixture Manalo‚ Ma. Cristina Joyce B. Department of Chemical Engineering‚ Faculty of Engineering University of Santo Tomas Espana‚ Manila A. Abstract Crude sodium bicarbonate or commonly known as soda ash may contain amounts of impurities like chlorides and hydroxides. The total acid neutralizing capacity of a soda ash sample‚ its alkalinity value‚ was stated in terms of mass of sodium carbonate. In doing so‚ any
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18 Instructor Explanation: To solve for ending work in process: + Work in process‚ beginning 30‚000 + Units started into production during the month 80‚000 - Units completed and transferred out during the month 85‚000 = Work in process‚ ending 25‚000 Equivalent Units of Production Materials Transferred to next department 85‚000 Ending work in process (conversion: 25‚000 units x 10% complete) 25‚000
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As more minors are committing violent crimes‚ (Greenwald‚ 1983) the question arises‚ Should minors who commit violent crimes be tried as adults? Based on the following scenario one would agree that minors who commit violent crimes should be charged as adults. In the case of a Middlesex teenager and his girlfriend‚ his punishment was severe and ultimately cost him his life. His girlfriend’s punishment was not quite as severe (unknown‚ www.clarkprosecutor.org‚ n.d.). According to the Journal of Criminal
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the heat transferred to the water. NOMENCLATURE HPWH – Heat Pump Water Heater SBHPWH – Solar Boosted Heat Pump Water Heater SBHP – Solar Boosted Heat Pump COP – Coefficient Of Performance SAHP – Solar Assisted Heat Pump DX-SAHP – Direct Expansion Solar Assisted Heat Pump Qw – Heat transfer to water Wc – Electricity consumed by the compressor CFC – Chlorofluorocarbon ODP – Ozone Depletion Potential GWP – Global Warming Potential transformed into higher grade heat and transferred to the
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SYNTACTICAL CLASSIFICATION OF PHRASEOLOGICAL UNITS Phraseological units can be clasified as parts of speech. This classification was suggested by I.V. Arnold. Here we have the following groups: a. noun phraseologisms denoting an object‚ a person‚ a living being‚ e.g. bullet train‚ latchkey child‚ redbrick university‚ Green Berets‚ b. verb phraseologisms denoting an action‚ a state‚ a feeling‚ e.g. to break the log-jam‚ to get on somebody’s coattails‚ to be on the beam‚ to nose out‚ to
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Combustion Of Alcohols Planning This investigation involves burning alcohol in the air. Key science- Chemistry by Eileen Ramsden says that " an alcohol is a series of organic‚ homologous compounds‚ with the general formula Cn H2n+1OH". The alcohol reacts with the oxygen in the air to form the products water and carbon dioxide: Cn H2n+1OH +(n+n/2)-1O2 ? nH2O + nCO2 The structure of the molecules in this reaction is: H H | | H - C - C - O- H + 3[O=O] ? 1/2[O=C=O] + 3[H-O-H] | | H H This reaction
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Drug overdoses are associated with significant morbidity and mortality. Cardiovascular-active medications are very challenging to treat in overdose situations. Intravenous lipid emulsion therapy (ILE) was first successfully used in local anesthetic systemic toxicity (LAST) in 2006. Since then there have been numerous case reports of ILE being used in other lipid soluble medication overdoses. We describe a case of a combined beta-blocker and calcium channel blocker overdose treated successfully with
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The sample became a cloudy white color. The reaction flask was connected to a reflux heating set-up and allowed to boil for thirty minutes until the cloudy white color dissolved into a clear liquid and then was cooled to RT. The sample was then transferred to a beaker and approximately 9 mL of sulfuric acid was added to the sample to adjust to pH 2. It was then placed in an ice bath to promote crystallization and then vacuum filtered to produce a white solid. Results and Conclusions – Reflux was
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Botany Essay Questions 1. Pollination is a crucial process of how plants reproduce‚ in which the male sex cell of the flower‚ pollen‚ is transferred from the stamen to the carpel‚ or more specifically‚ from the anther to the stigma. Pollen grains are completely different and unique for each flower‚ they carry the male and female genetic information and it is split exactly 50/50. The transfer is carried out by pollinators‚ which include bees‚ wasps‚ flies‚ butterflies‚ moths‚ several species
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