Abstract Some bacteria are able to go through transformation making new combinations of genes. Transformation is a way of gene variability in bacteria. This experiment is based on the transformation mechanism of bacteria and gene regulation. The bacteria used for the experiment was Escherichia coli and the genes introduces for the transformation were: gfp and bla by a pGLO™ plasmid. After the insertion of the target genes and growing the bacteria on specialized LB media‚ it could be seen that the
Premium DNA Bacteria Gene
“He became as good a friend‚ as good a master‚ as good a man as the good old city knew…” Discuss Scrooge’s transformation in A Christmas Carol. In Charles Dickens’ novella ‘A Christmas Carol’‚ Ebenezer Scrooge’s transforms himself from an undesirable‚ self-centred old man to a gentleman with characterisations to exemplify that the most valuable things in life are those which are not tangible; family‚ friends‚ love and happiness. The protagonist had the ability to help the unfortunate and deprived
Premium Charles Dickens Ebenezer Scrooge Bob Cratchit
The call by some senior members of the African National Congress for the transformation of the Judiciary doesn’t compromise judicial independence Introduction In the trias politica‚ every arm of government has equal power compared to other arms of the government. However‚ to make sure that all the arms of government in the trias politica have equal powers they must be accountable to each other. That means they must implement the oversight to one another so that no arm of government ends up with
Premium Separation of powers Executive Judiciary
Many works of art have hidden influence in them stemming out from all parts of the world. With these influences come themes tied to each type of work. The Warrior and Transformation Mask are just a couple examples of this. The Warrior was created in between 900-400 BC‚ created by the Olmec‚ it was one of the many giant heads found all over the Olmec area usually brought over land and along rivers. They are thought to be created to honor specific rulers and are a testament to his power. Approximately
Premium Mesoamerica Ancient Egypt Aztec
Transformation of E. coli with your unknown plasmid Learning Goals: Insert your uncut unknown plasmid into chemically competent DH-5 E.coli cells and use antibiotic resistance to confirm the success of the transformation. You should familiarize yourself with the various methods of transformation and the advantages/disadvantages of each type. You should also understand how heat shock transformation works and how chemically competent cells make this type of transformation possible. For this transformation
Premium Molecular biology DNA
Healthcare Delivery and Financing By: Chrishanda Anderson HCMT241/ Rm. 1035 February 1‚ 2014 Introduction In this paper I will explain and discuss the healthcare delivery system and the financial aspects of it. This paper will also include resources and examples that will help to understand and explain the overall delivery and financing of healthcare. The topics that will be covered in this paper are and overview of the healthcare delivery
Free Managed care Health care Preferred provider organization
PERSONNEL PSYCHOLOGY 2011‚ 64‚ 225.262 DOING COMPETENCIES WELL: BEST PRACTICES IN COMPETENCY MODELING MICHAEL A. CAMPION Purdue University ALEXIS A. FINK Microsoft Corporation BRIAN J. RUGGEBERG Aon Consulting LINDA CARR Sun Microsystems GENEVA M. PHILLIPS RONALD B. ODMAN Boeing Company The purpose of this article is to present a set of best practices for competency modeling based on the experiences and lessons learned from the major perspectives on this topic (including applied
Premium Organizational studies and human resource management Industrial and organizational psychology Human resource management
Brittany Exam 3 Essay 9 Dec 2012 Transformations of the American West The three most important transformations of the American West were the completion of the railway system‚ economically‚ the diversity of the people‚ socially‚ and the conservation of land‚ politically. All of these transformations have made an unmistakable impact of the American West and what it is today. The largest economic transformation in American Western history was the building of the railroad systems. It was the
Premium American Old West Native Americans in the United States Cowboy
L O G I L I T Y V O YA G E R S O L U T I O N S An Executive White Paper Five Inventory Core Competencies That Can Make or Break Your Competitive Advantage Key Best Practices in the New Inventory Optimization Center of Excellence L O G I L I T Y V O YA G E R S O L U T I O N S An Executive Whitepaper Table of Contents Five Inventory Core Competencies That Can Make or Break Your Competitive Advantage Evolution of Inventory Optimization................2 Key Best Practices
Premium Inventory
Healthcare in America Abstract Healthcare has become one of the largest social problems in the United States. Healthcare costs are rising and at the same time preventative care is spiraling downward. Healthcare has become unattainable for those who are financially disadvantaged or those with chronic illnesses and unaffordable by those that are already insured. The United States is fast becoming one of the worst health care systems in the world. Not only are they the only industrialized
Premium Health care Medicine Healthcare reform