Transformation of E. coli with your unknown plasmid Learning Goals: Insert your uncut unknown plasmid into chemically competent DH-5 E.coli cells and use antibiotic resistance to confirm the success of the transformation. You should familiarize yourself with the various methods of transformation and the advantages/disadvantages of each type. You should also understand how heat shock transformation works and how chemically competent cells make this type of transformation possible. For this transformation
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In most novels a motif represents one thing‚ in Jane Eyre the motif of fire changes as Jane gets older‚ more mature and meets new people. In the beginning of the novel fire represents comfort to Jane. This changes to passion as Jane gets older and meets Mr. Rochester‚ When Jane is young fire represents comfort even in places she does not like or feel comfortable like Gateshead or lowood. During her time at gateshead jane was sent to the red room from time to time as punishment. Jane was very afraid
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To metamorphose ones character through years of experience and age is salutary. To deteriorate ones character through a short period of fast decisions and unsure actions is perilous. Lady Macbeth proves the truth to this theory. The impulsive mistakes and power-hungry tactics littered the journey Lady MacBeth paves throughout this play that ultimately ends in her death. She feels overwhelmed by all that is happening‚ both physically and mentally‚ and decides to end her own life. The Tragedy of Macbeth
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Brittany Exam 3 Essay 9 Dec 2012 Transformations of the American West The three most important transformations of the American West were the completion of the railway system‚ economically‚ the diversity of the people‚ socially‚ and the conservation of land‚ politically. All of these transformations have made an unmistakable impact of the American West and what it is today. The largest economic transformation in American Western history was the building of the railroad systems. It was the
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Running head: ORGANIZATIONAL TRANSFORMATION Q & A Organizational Transformation Q & A Jose Luis Sandoval Jr. OI/361 December 1‚ 2014 Michele Dougherty 1 ORGANIZATIONAL TRANSFORMATION Q & A 2 Organizational Transformation Q & A How incentives‚ training‚ and education are important to a company and also how important a strong leader is to a company. Jose will define and explained these topics. He will also compare these topics to his previous employment (Gill’s Auto Glass) and explain the positive
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My Preference of The Doctor’s Tale The tale that I selected to read was the astronomy practicing Physician. This story of love‚ in hatred and bigotry was extremely interesting to read however‚ on the same note the wording was tremendously derived in Middle English tone which made a few sections difficult to understand and comprehend. The non-bible reading doctor’s tale is interesting from beginning to end‚ he paints a vivid picture of all his characters and there attributes using immense direct
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Jayanthi 1! Sishir Jayanthi Mrs. Eve Itaya H. English 10 P.2 14 February 2015 Mental Deterioration Shakespeare uses the motif of mental deterioration to convey the point that achieving one’s ambitions through malicious deeds can cause psychological collapse. Psychological deterioration through wrong doings is first evident before Macbeth murders Duncan. “Is this a dagger I see before me” (cite). Macbeth questions himself‚ before he is about to kill Duncan; it could be interpreted that whilst the
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Bacterial Transformation Lab Introduction: In this experiment we transformed a strain of E. Coli bacteria without antibiotic resistance with plasmid DNA. This plasmid produces a fluorescent green glow under black light due to the gfp(green fluorescent protein) as well as antibiotic resistance. E. Coli cells will be plated on an agar medium‚ some with and some without the antibiotic ampicillin. Only bacterial cells that contain the plasmid will survive the ampicillin and produce the green glow
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The State of Mind: Symbolized Motifs and Symbolism in “The Great Divorce” Everyday there is a sun. The sun rises and the sun sets. When the sun rises there is a beautiful glow to it yet very few watch it rise because it is so early. But when it sets many people think of it as the end of the day and fantasize on its romanticism into the night‚ pondering on achievements throughout the twenty-four hour time period. The sun is a symbol of life. Much like the sun‚ the symbols in the Great Divorce are
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Motif Analysis: Blood Blood is everywhere in Macbeth. The very first scene of the play starts out with a captain speaking of the blood spilled at the battle between the Scots and the Norwegians. Those this is the first instance of blood; blood plays a much bigger part in Macbeth. Blood stands for the guilt that Macbeth and Lady Macbeth feel throughout the play. While Lady Macbeth is planning the murder of King Duncan‚ she calls upon the spirits of murder to‚ "make thick my blood; / Stop up the
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