Vector in Genetic Transformation Introduction: In this experiment we are testing what is required for E. coli to successfully grow on LB (Luria Broth) plates with ampicillin and determining if any genetic transformation has occurred. By combining +pGLO LB and ampicillin we should get an ampicillin resistant gene and by using –pGLO we should create a non-genetic resistant bacteria. The pGLO plasmid has the GFP (green fluorescent protein) gene and the gene that allows the plasmid to be resistant
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Biology101 McDowall 10/29/2012 Escherichia Coli “The Bacterial Beast” E.coli or binomial name Escherichia coli was discovered by a German pediatrician named Theodore Escherich in 1885. Dr. Escherich originally named the bacteria‚ bacillus communis coli. After the demise of Dr. Escherich the intestinal bacteria was then named Escherichia coli after the late doctor in 1919. The bacteria Escherichia coli are classified as follows. Domain- Bacteria Kingdom- Eubacteria Phylum- Proteobacteria
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Title of the lab: Transformation : Bacterial Genetics Purpose of the lab: The pupose of the lab was to transfor a bacterial E. Coli by using the green flurescent protein from the jellyfish. Another important that was fferdone by making the cell competency‚ meaning that it will be able to take on additional DNA. This was done when the plasma was added. Materials: 1. 37 o C water bath 2. Ice 3. Sterile transfer pipette 4. Foam tube rack 5. Transformation solution (CaCl2) 6. pGLO plasmid 7. Sterile Inoculating
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Transformation in bacteria is the genotype alteration by the uptake of naked‚ foreign DNA from the environment. This concept of transformation was first discovered when Fred Griffith an experiment using mice and strains of pneumonia. Griffith concluded that a “principle” was transferred from heat-killed S strains to the R strains‚ which transformed them into deadly S strains. Oswald Avery later determined‚ through a series of experiments‚ that DNA was the “principle” that caused the R stains to become
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In this lab‚ we performed a genetic transformation through the process of gene transfer. Gene transfer involves the insertion of a gene into an organism. The gene to be inserted is usually contained in a plasmid‚ which is relatively small‚ circular non-chromosomal DNA molecule typically found in bacteria. Once the plasmid containing the gene is inserted into the organism‚ it is absorbed into the organism’s own genetic code. After this occurs‚ the newly introduced gene begins coding for proteins‚
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Life of Escherichia Coli Escherichia Coli 0157:H7 bacterium can live in the intestines of cattle‚ therefore meat can become infected during slaughtering. You can contract this bacterium by eating undercooked meat‚ especially ground beef. Escherichia Coli 0157:H7 can also be contracted by eating contaminated fruits or vegetables‚ as well by drinking raw fruit juices or milk‚ or sewage contaminated water. Infection can also be spread via person to person. Escherichia Coli 0157:H7 can also be
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Introduction Transformation is a genetic alteration of a cell resulting from the direct uptake and incorporation of exogenous genetic material from its surrounding through the cell membrane. The arabinose operon changes AraC from a repressor to an activator; in this experiment the pGLO plasmid has been designed with a modified operon so that in the presence of the arabinose the bacterial cells which have been transformed by the pGLO plasmid will fluoresce due to the production of GFP. SDS-PAGE
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Genetic transformation happens when an organism is altered by the introduction of new genetic information which is merged into the organism’s genome. Bacterial transformation is a type of genetic transformation that was used in lab and mainly used due to the single celled nature of bacteria. In this lab‚ the engineered pGLO plasmid is integrated into E. Coli bacteria‚ and adds the genes which code for the proteins GFP in the modified bacteria’s genome (Hanahan‚ Studies on transformation of Escherichia
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NA transformation of E. coli: The two plasmids were added to individual tubes containing E. coli and one with no plasmids. The three samples of E. coli were heated in a 42°C water bath for 90 seconds to heat shock the bacteria so that the plasmids would be taken up by the E. coli. These samples were then incubated at 30°C for half an hour and then plated on LB agar. Each tube was plated on an LB plate and a LB + ampicillin plate. Ampicillin is an antibiotic that is effective against E. coli‚ both
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BACTERIAL TRANSFORMATIONS USING PVIB II. INTRODUCTION Transformation is the manipulation of a bacterial cell’s DNA in order to alter the cell’s genotype or phenotype by absorbing free DNA from its surroundings. In this lab‚ pVIB plasmid will be used. A plasmid is a segment of DNA that can incorporate itself into the bacterial DNA. Although is not required for growth of the bacterial cell‚ plasmids can provide advantages in stressful environments such as the ability to adapt as environmental
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