Transformation of E. coli with your unknown plasmid Learning Goals: Insert your uncut unknown plasmid into chemically competent DH-5 E.coli cells and use antibiotic resistance to confirm the success of the transformation. You should familiarize yourself with the various methods of transformation and the advantages/disadvantages of each type. You should also understand how heat shock transformation works and how chemically competent cells make this type of transformation possible. For this transformation
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Brittany Exam 3 Essay 9 Dec 2012 Transformations of the American West The three most important transformations of the American West were the completion of the railway system‚ economically‚ the diversity of the people‚ socially‚ and the conservation of land‚ politically. All of these transformations have made an unmistakable impact of the American West and what it is today. The largest economic transformation in American Western history was the building of the railroad systems. It was the
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Running head: ORGANIZATIONAL TRANSFORMATION Q & A Organizational Transformation Q & A Jose Luis Sandoval Jr. OI/361 December 1‚ 2014 Michele Dougherty 1 ORGANIZATIONAL TRANSFORMATION Q & A 2 Organizational Transformation Q & A How incentives‚ training‚ and education are important to a company and also how important a strong leader is to a company. Jose will define and explained these topics. He will also compare these topics to his previous employment (Gill’s Auto Glass) and explain the positive
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English 126 29 November 2012 Joyce Carol Oates‚ The Author of "Where Are You Going‚ Where Have You Been?” Many critics have found that the possibility of the psychological changes of a teenager is a discussable topic to learn and argue about. Connie‚ the young teenage girl in the story of “Where Are You Going‚ Where Have You Been?” faces an unpredictably-dangerous
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According to the supplemental material‚ Carol Gilligan identified that males see themselves as separate from others whereas females see themselves in terms of their relationship with others. Therefore‚ Jon always placed himself outside of his peers and never within. Jon felt that his peers classified him as not being ‘normal’. Jon defined the term being ‘normal’ as an individual that “get drunk‚ go on dates‚ not do any homework‚ hang out‚ and just love living life without a care in the world” (Garrod
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Hanukkah and Christmas Happy Hanukkah! Merry Christmas! Have you ever heard those phrases before? Children say it to their parents‚ teachers‚ friends‚ and their neighbors. December has several holidays in it‚ but everyone kind of knows the two which is Hanukkah and Christmas. Hanukkah is the eight-day Jewish celebrates the re-dedication during the second century B.C of the second temple in Jerusalem. Christmas is celebrated on the twenty-fifth of December in honor of the day Jesus was born. Both
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Bacterial Transformation Lab Introduction: In this experiment we transformed a strain of E. Coli bacteria without antibiotic resistance with plasmid DNA. This plasmid produces a fluorescent green glow under black light due to the gfp(green fluorescent protein) as well as antibiotic resistance. E. Coli cells will be plated on an agar medium‚ some with and some without the antibiotic ampicillin. Only bacterial cells that contain the plasmid will survive the ampicillin and produce the green glow
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and in position C26 have been reported. 11 α hydroxylation is regarded as essential for anti-inflammatory action. Present study was promoted to extend the investigations to evaluate potentiality of this local oil contaminated soil isolates for transformation of progesterone. Enrichment is carried out in the inorganic medium providing progesterone as an only carbon source. Metabolites are identified by GC/MS and isolates by 16 s rRNA. 11 α hydroxyprogesterone is the metabolite identified having 60
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Dustin Johnson Dr. Pen Pearson English 201 20 September 2010 Lloyd Christmas is a limo driver. The movie begins when Lloyd gets in the back of his limo and rolls down the window to ask a woman a question. Lloyd‚ “Excuse me. Could you tell me how to get to the medical school? I’m supposed to be giving a lecture in 20 minutes‚ and my driver’s a bit lost.” Lady at bus stop‚ “You go straight ahead and make a left over the bridge.” Lloyd‚ “That’s a lovely accent you have. New Jersey?”
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all began when Christmas break started. Just like other kids‚ I was extremely excited for the break. It meant no school for two weeks‚ presents‚ and more importantly‚ no school for two weeks. I didn’t know what I wanted for Christmas that year. I had everything‚ it seemed. I had entertainment‚ a family that loved me‚ good grades‚ and a hobby that I loved: soccer. The only thing I could think of getting was the new DSi. I had a ds‚ but how could an upgrade hurt anyone? So as I added it to the list
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