microliter). The solution was left for 20 mins at 30˚C (RNase A Treatment). Then it was centrifuged at 15000 RPM for 10 minutes to remove any debris. The supernatant was incubated with an appropriate amount of flag- crosslinked beads at 4˚C for three hours‚ rotating. Afterwards‚ the beads-containing solution was centrifuged
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Aircraft structures Abstract: The aircraft structure is an important part where the structure of an aircraft depends on the optimum requirement of the customer and the environments in which it will be going to fly for the major time. It has to go several checks at regular interval of flying hours under proper inspection. This paper contains the basic information regarding the components of an aircraft and the basic concept of its design. The main criteria of design and its philosophy is the
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Title: An investigation into the impact of group pressure on an individual’s estimate of the amount of beads in a pot (ginger granules in a jar). IV = Group/Individual DV = Individual beads estimate Abstract This experiment investigated the impact of group pressure on the individual. The hypothesis is that group pressure does indeed impact on the individual and in this case the individual’s estimate of the number of ginger granules in a jar. Participants were asked to make a judgement of
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some of their parents must be rich‚ while some may be in poor economic condition. Thus‚ the teacher would receive all kinds of gifts which vary from a DIY card to a card worth thousands yuan. On this occasion‚ students who send cheap things like trinket may feel ashamed and distance themselves from the teacher. Moreover‚ many parents want the teacher to pay more attention to their children by giving special presents. Such behavior is similar to ‘bribery’‚ which is unfair. And some teachers are likely
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Title: Purification of Green Fluorescent Protein Introduction: Transformation is used to introduce a gene coding for a foreign protein into bacteria. Hydrophobic Interaction Chromatography (HIC) is used to purify the foreign protein. Protein gel electrophoresis is used to check and analyze the pure protein. Research scientists use Green Fluorescent Protein (GFP) as a master or tag to learn about the biology of individual cells and multicultural organisms. This lab introduces a rapid method
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Slavery is a concept that has circulated the world more than enough times to count. It has impacted many people along the way and has made peoples’ lives for the worst. Although not everyone experiences it‚ those who have are silences and are unheard of. In the graphic history book Abina and the Important Men‚ it demonstrates the story of a women of color and the court case she went through with her former slave owner. It shows how people forms different ideas and the consequences it brings to certain
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Chapter 4 Downstream Processing Objectives Student should be able to Define filtration‚ centrifugation‚ cell disruption‚ solvent extraction‚ chromatography‚ membrane process‚ action of force field‚ evaporation & drying Explain briefly on the concept of each topics stated above List out the uses & importance of each topic stated Introduction Each stage in the overall recovery procedure is strongly dependent on the history & quality of the biological production process. Maximization of
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Drew Carter Hensley English 11 Honors 2nd Period Hills Like White Elephants Summary The story begins in a valley in northern Spain near the Ebro river at a train station surrounded by hills‚ fields‚ and trees. An American man and his girlfriend‚ who he calls ?Jig?‚ are waiting for a train coming from Barcelona in forty minutes that would stop at the station and go to Madrid. While waiting in the shade at a table outside the building‚ the two discuss what they will have to drink. The man points out
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believed to have been performed by warriors on their return from battles.. The male dancers wear large feathers as part of their headgear‚ hold an ornate and long shield in their hand with chains‚ beads and a loincloth called the ‘cawat’. The female dancers have an elaborate headdress‚ chains‚ beads and a ‘dress’ that reaches to below their knees with intricate weaving. Traditionally this dance was only performed by male dancers but not anymore.The dance is arranged straight lines and in a circle
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Source 1 Source 1 Animal skin Animal skin Boxes like living caskets Boxes like living caskets Harsh stitching Harsh stitching "Atrabiliarios‚" 1992-93 Shoes‚ animal fiber‚ and surgical thread‚ dimensions variable Collection of The Pulitzer Foundation‚ St. Louis‚ Missouri Photo by Robert Pettus © Doris Salcedo "Atrabiliarios‚" 1992-93 Shoes‚ animal fiber‚ and surgical thread‚ dimensions variable Collection of The Pulitzer Foundation‚ St. Louis‚ Missouri Photo by Robert Pettus
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