Restriction Endonuclease Digestion of DNA from E. coli cells and Analysis by Agarose Gel Electrophoresis Introduction The main goals of this experiment are testing an alternative procedure called “boiling lysis”‚ evaluating the quality of the purified plasmid for restriction digests‚ and identifying the mislabeled plasmid. The plasmid DNA from a carrier E. coli strain was purified by the boiling lysis. In the boiling lysis method‚ the bacterial cells were given momentary heat treatment
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Another heroine who does not stick to the tired gender conventions is Beatrice ’Tris’ Prior from Divergent. Divergent focuses on Beatrice and her development not only as a hero but also as a female who is beyond the gender stereotypes. The movie was directed by Neil Burger and released in 2014; it is also one of the movies from past few years with a female as the main character which turned out to be successful. Beatrice lives in a dystopian future in Chicago which is divided into five factions
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“My first instinct is to push you until you break‚ just to see how hard I have to press‚ he says‚ his fingers squeezing of the word ‘break!’ My body tenses at the edge in his voice‚ so I am coiled as tight as a spring‚ and I forgot to breathe. His dark eyes lifted to mine‚ he adds‚ ‘but I resist it.’ ‘Why… I swallow.’ ‘Why is that your first instinct?’ ‘fear doesn’t shut you down; it wakes you up. I’ve seen it. It’s fascinating.’” In this scene‚ Tobias brings about the importance of how fear means
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the rest of your life would be chosen based on a technological simulation? How would you feel if you had to choose either bravery‚ knowledge‚ service‚ honesty‚ or peace? What if the choice you made meant no going back and maybe even no family? Join Tris as she makes the ultimate choice. In a future dystopian Chicago‚ society is broken into five factions: Candor‚ Abnegation‚ Dauntless‚ Amity‚ and Erudite‚ each specializing in a specific trait. Children are brought up in their own factions‚ learning
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functional groups‚ if any‚ and naming them by the their ion names (such as hydroxy for -OH‚ oxy for =O‚ oxyalkane for O-R‚ etc.). Different side-chains and functional groups will be grouped together in alphabetical order. (The prefixes di-‚ tri-‚ etc. are not taken into consideration for grouping alphabetically. For example‚ ethyl comes before dihydroxy or dimethyl‚ as the "e" in "ethyl" precedes the "h" in "dihydroxy" and the "m" in "dimethyl" alphabetically. The "di" is not considered in
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Akin‚ Esq. RE: Recommendation Regarding the Tri Start Wide-body Aircraft Project Date: 1971 early 72ish Recommendation: The L-1011 project should be canceled. Cancelling the L-1011 project will increase shareholder value by $161.92 per share. Excluding preproduction sunk costs‚ including a cost of capital 16%‚ and sales of 113 units and a per unit sales price of $16 million‚ the net present value (NPV) of continuing with the Tri Star is -$ 1‚829‚666‚246. Rationale for Decision:
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Buffer: to prepare 50 mL of buffer dissolve 23.6 g of Guanidine thiocyanate (118.16 g/L) in 25 mL of double-distilled water. Once dissolved add: 2.5 mL of 1 M Tris-HCl pH 7.0 (121.14 g/L)‚ 2 mL of 0.5 M EDTA pH 8.0 (186.12 g/L) and 0.05 mL of Triton X-100. Add double-distilled water to 50mL (final concentrations: 4 M guanidine thiocyanate‚ 50 mM Tris-Hcl pH 7.0‚ 20 mM EDTA and 0.1 % Triton X-100). 36. DNA Binding Solution: First‚ wash diatomaceous earth (silica particles) by resuspending 5 g in 50 mL of
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Publisher’s Weekly’s Best Book Of 2011 Text Type: Novel _____________________________________________________________________ The novel “Divergent” written by author Veronica Roth is a thrilling story about the love and sacrifice of two teenagers (Tris and Four) living in dystopian America. This novel follows the hardship of citizens living in a community where everyone must be separated into groups called factions. The members of each faction must live their lives according to the value of their
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We examined the decision to invest in the Tri-Star project by forecasting the cash flow associated with the project for a volume of 210 planes. We also asked what a valid estimate of the NPV of the Tri-Star project at a volume of 210 planes as of 1967 would be. We found this to be -$584 M. This was clearly an unacceptable NPV for capital budgeting on the project. A break-even analysis revealed that the project reached economic break-even with the production of 275 planes at $12.5 M per unit but
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suicide into the chasm Born Faction: Candor Chosen Faction: Dauntles Beatrice Prior ("Tris" is the main character and narrator) Hair color: Blond Eye color: Blue-gray Physical description: Dull blond hair twisted in a knot or braided‚ narrow face‚ large wide round eyes‚ long thin nose‚ blue/gray eyes‚ small frame. “I still look like a little girl‚ though sometime in the last few months I turned sixteen.” Tris gets three tattoos: three ravens above her collar bone representing her three family
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