magnification of a high-powered microscope. These techniques of microbiology are used in the following five experiments. The first experiment used Trypticase Soy Nutrient Agar (TSA)‚ which can grow a wide variety of organisms and contains casein and soybean meal and a minute NaCl‚ to study the effectiveness of alcohol as a skin antiseptic. The second experiment tested the effectiveness of different kinds of mouthwashes as antiseptics using TSA as well. Experiment number three explored the normal human
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to inoculate a TSA petri plate by doing a quadrant streak‚ which is aseptically done by making an initial streak‚ then sterilizing and repeating by streaking out of one portion of the previous streak no more than the initial streak. This was done a maximum of four times‚ insuring that the bacteria culture is "thinned" out enough to take a sample from an individual colony. This was repeated one other time and both were refrigerated at 37 degrees for a minimum of 24 hours. Each plate was labeled with
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Unknown Lab Report Microbiology Unknown A Sonia Kabra November 26‚ 2014 Introduction There are numerous reasons for identifying unknown bacteria. Some of these organisms have distinct qualities that set them apart from one another‚ such as the exposure to certain environments. Through out the semester in the laboratory‚ we are able to encounter some of the few microorganisms that we as humans have come into contact with. With the knowledge gained from the sessions in the laboratory‚ we can now
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Stevens‚ S.K.‚ & Weber‚ R.A. Bacillus Cereus Fact Sheet (2013) Food Safety Counsel. Retrieved from http://www.foodsafetycounsel.com/food-safety-law/common-food-borne-pathogens/bacillus-cereus/ Todar‚ K Dichotomous Key‚ Coastline Community College Microbiology Lab. Personal photograph by author. 2013. EMB Positive and Negative results. Digital image. Flickriver.com. Flickriver Viewer Software‚ 2007. Web. 3 Nov. 2013. An endospore stain of Bacillus cereus using malachite green. Digital image. Academic
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this exercise I needed a TSA plate and labeled the bottom of it with my name‚ group number and organism. I also divided the bottom into three sections. After that‚ I sterilized my inoculating loop using the bunsen burner flame. After letting the loop cool‚ I reached into my unknown test tube with my loop and grabbed the organisms. After that I streaked the top 1/8th of the plate then sterilized the loop. After letting the loop cook I streaked the second section of the plate while crossing into the
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Microbiology Lab Report II Title: Determination of a Bacteriophage Titer Purpose: To determine the number of phage particles or plaque-forming units in a suspension of T4 bacteriophage. Materials: 18 24 hour broth culture of Escherichia coli B. 2 ml suspension of T4 bacteriophages with a titer of at least 10‚000 phages/ml 5 trypticase soy agar (TSA) plates. These should be warmed to 37c before use 5 tubes of soft agar (0.7% agar). Prior
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cultures were Gram stain‚ Catalase‚ Mannitol Salt Agar (MSA)‚ Blood Agar‚ Novobiocin‚ Coagulase‚ and DNAse (Alachi‚ 2007). Rebekah Worley February 21‚ 2012 Mitchell Section 4 Biol 311 Staining and Identifying Unknown Bacteria Introduction: The microbiology lab up to this point has been used to teach the students how to stain and identify bacteria. There are several types of staining through which the bacteria can be identified based on the color and shape. The staining methods used in the lab are
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Aseptic Technique and Streak Plates Purpose The purpose of this exercise is to learn aseptic technique procedures and there importance and also to learn to isolate colonies using the streak plate technique. Introduction Bacteria are inoculated (introduced) and cultured (grown) in the laboratory for test studies to determine their morphology (the shape‚ size‚ arrangement‚ and internal structures) and pathology (ability to cause disease). Inoculation has to be performed without adding other microbes
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MICROBIOLOGY 3444-007 Gram Negative Unknown Unknown Bacteria #14 LeNaiya Kydd 4/2/2014 Abstract In order to be able to identify the unknown organism that was given to us‚ we had to conduct a number of different tests. These biological tests are used because they help us be able to identify the properties of the unknown we have and be able to compare our observed results with actual results of all the potential organisms. When all of the data of the test are put together it is easy to
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in Microbiology‚ and use it to collect and examine microbial species from the physical environment. The physical environment around the lab will be utilized to find out the variety of different microbial species growing in and around the lab. This investigation will help in preparation for the individual projects that will be done at the end of the semester. Materials and Methods: The lab chosen was Exercise 24: Isolation of Bacteria in our Physical Environment. For this lab‚ four TSA Petri
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