Gram Negative Unknown Lab Report Abstract The Unknown Gram Negative bacterium inoculated in a Tryptic Soy broth medium was randomly selected from a group of other unknowns. In order to identify this unknown the seven different types of biochemical tests will be conducted on this unknown bacterium to identify it out of 6 possible bacteria; Escherichia coli(E. coli)‚ Enterobacter aerogenes (E.aerogenes)‚ Klebsiella pneumoniae (K.pneumoniae)‚ Proteus mirabilis(P.miranilis)‚ Pseudomonas aeruginosa(P
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everywhere all the time. In this lab you will test the idea that microbes are ubiquitous or present everywhere at all times. Materials One Triptic Soy Agar (TSA) plate per student One sterile swab Procedure 1. Decide on an area to test for the ubiquity of microbes. List your area on the white board in front of the classroom. 2. Obtain one TSA plate and label it on the bottom (side with the agar) with your name‚ class section and the surface you will sample. 3. Obtain one sterile swab. 4. To obtain
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lab‚ inoculation‚ incubation and inspection are used. Inoculation is used because each TSA (tryptic soy agar) media was placed in a different place to collect cells as well as printed with a thumb print from each member of the group. Incubation is used because each sample needed a certain temperature for the cells to grow. Inspection was used to look at each plate and see how many colonies are found. One TSA plate was placed wherever the student wanted in the building and the other was for each group
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I began using the streak plate technique by diving the medium into four quadrants with a wax penicil. Then I used the aseptic technique to transfer a small amount of bacteria with my inoculating loop to the first quadrant. Still using the loop‚ I streaked the bacteria across the
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swab to collect our samples. We started by thoroughly swabbing the top-side of the middle toilet seat in the women’s restroom and taking a separate swab from the keypad of the ATM machine. We them transferred our collected sample onto two separate TSA plates by swabbing the agar in a zigzag fashion‚ rotating the petri dish to spread the bacteria in various directions. This culturing technique helped us to see the full growth of the biofilm that would be growing after inoculation. After the fifth day
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Corynebacterium diphtheriae‚ Nocardia farcinica‚ Rhodococcus equi‚ Streptomyces griseus‚ GRAM NEGATIVE--Escherichia coli‚ Salmonella typhi‚ Vibrio cholorae‚ Heliobacter pylori‚ and Shigella dysenteriae . Pseudomonas aeroginosa is needed to be grown on a TSA plate to get be able to get a dry extract from. The first step would be to have the dry extract of pseudomonas at the bottom of the capillary tube and then to stick the capillary tube into a test tube where there is a live broth culture of a certain bacteria
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The unknown bacteria A and bacteria B have to be identified by its genus and species. First both bacteria had to be inoculated into a TSA agar media using the streak plate method. Four quadrants were drawn‚ so that the bacteria could be isolated as much as possible. Each bacteria was inoculated into two different plates‚ so that one could be incubated at 37 degrees Celsius and the other at 25 degrees Celsius. Bacteria B‚ which was incubated at room temperature showed red colonies throughout its media
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Introduction: Biological organisms are classified uniformly in order to easily categorize and identify organisms. This classification‚ or taxonomy‚ uses the genus name followed by the species name‚ in Latin. By having a universal method of identifying bacteria allows for all scientists from any part of the world to identify the same species in an identical manner allowing for a precise of classification. Bacteria are distributed throughout the world in almost every conceivable habit. Bacteria are
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differing levels. CULTURES Pseudomonas aeruginosa or Micrococcus luteus‚ Clostridium‚ Escherichia coli (Aerobes) (Anaerobe) (Facultative anaerobe) MEDIA 3 TSA plates (for anaerobic chamber) CULTURE CONDITIONS • Lightly streak 4 quadrants on TSA agar for anaerobic chamber. Make sure you can’t see bacteria
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Running head: DOMESTIC SURVEILLANCE 1 DOMESTIC SURVEILLANCE Marianna Dannelley March 26‚ 2014 Running head: DOMESTIC SURVEILLANCE 2 Have you ever wondered if you ever being watched? Almost like every time you go through a stop light or at an ATM machine‚ that someone knows your every move? Well they
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