Pseudomonas taiwanensis – Enrichment‚ Isolation and Identification Microbiology 521 2/10/12 Purpose: The purpose of this experiment was to enrich Pseudomonas bacteria‚ isolating a species of Pseudomonas and identifying it using phenotypic properties and DNA sequencing as an existent or completely new and undiscovered species of Pseudomonas. Overview: Genus Pseudomonas is a chemoheterotrophic bacteria found in soil and water. They are Gram negative‚ motile‚ paired rods that are also oxidase-positive
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and I most certainly expect my results to prove so. I expect to find many shapes‚ sizes‚ colours and indeed types of microorganisms present when I observe the morphology of the colonies present. Materials and methods: Swabs. Diluent 0.1% peptone. TSA (Trypticase
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Methods In this lab‚ we use the procedure from Chemically Defined‚ Complex‚ Selective‚ and Differential Media. We divide the agar plates that have the unknown in third‚ write our initials‚ and date on the bottom of the agar plate. We also divide the agar plates into quadrants‚ label each organism in their quadrant‚ write our initials‚ and date on the bottom of the agar plates. We flame the loop and let it cool for a minute‚ we remove the cap of each culture tube of organisms with our pinky while holding
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(microaerophilic) and the LSD plates for the cabbage in our experiment (Foster‚ 2009). The bacterial counts were the highest for the lactic acid producing bacteria. The initial salt saturation of 5% with the tap water. The lactic acid bacteria can tolerate the salt and it help deter the unwanted bacteria and aid in the production of acid. The following predictions for the five plates are as follows‚ the TSA plate will gradually increase and then plateau. Also‚ the WN5 plate would increase over time
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obtained from various sources. Two bacterial colonies were isolated from plates that were incubated in the lab and these bacteria became the basis for this project. The bacterial cultures were maintained and used for various physiological‚ cultural‚ and biochemical tests and observations over several weeks. The purpose of culturing bacteria over half the course of the semester was to learn various techniques used in microbiology labs and to subsequently utilize those techniques for the purpose of identifying
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Lab 11 Methodology By using aseptic‚ a little cultured bacteria was inoculated on the TSA agar. A quadric streak was making. Inoculation loop was heated and keep it cold for a while before the next quadratic streak. Six agar plates were observed for 24 hour at temperature of 30ºC. Choose one from the dense colony and make a sub-culture on the new agar plate. The step was repeated to get a single colony‚ which is pure colony. a) Sequestration of bacteria from fish organs Methodology
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For example‚ the cabbage showed no growth over the course of the 14 day period on both the EC and PS plate‚ which is a desirable sign that the produce could be eaten. The EC plate is selective towards enteric bacteria‚ and the PS plate is selective to the species Pseudomonas‚ both of which are not ideal for consumption. Whereas‚ the data for the cucumber during the 14 day fermentation process showed growth on the PS
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Gram Negative Unknown Lab Report Abstract The Unknown Gram Negative bacterium inoculated in a Tryptic Soy broth medium was randomly selected from a group of other unknowns. In order to identify this unknown the seven different types of biochemical tests will be conducted on this unknown bacterium to identify it out of 6 possible bacteria; Escherichia coli(E. coli)‚ Enterobacter aerogenes (E.aerogenes)‚ Klebsiella pneumoniae (K.pneumoniae)‚ Proteus mirabilis(P.miranilis)‚ Pseudomonas aeruginosa(P
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everywhere all the time. In this lab you will test the idea that microbes are ubiquitous or present everywhere at all times. Materials One Triptic Soy Agar (TSA) plate per student One sterile swab Procedure 1. Decide on an area to test for the ubiquity of microbes. List your area on the white board in front of the classroom. 2. Obtain one TSA plate and label it on the bottom (side with the agar) with your name‚ class section and the surface you will sample. 3. Obtain one sterile swab. 4. To obtain
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lab‚ inoculation‚ incubation and inspection are used. Inoculation is used because each TSA (tryptic soy agar) media was placed in a different place to collect cells as well as printed with a thumb print from each member of the group. Incubation is used because each sample needed a certain temperature for the cells to grow. Inspection was used to look at each plate and see how many colonies are found. One TSA plate was placed wherever the student wanted in the building and the other was for each group
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