agar (TSA) was poured into six groups of 60 Petri dishes (See Appendix 1). The dishes were labeled based on the antibiotic used and were left to dry and solidify at room temperature. After an hour‚ the dishes were placed in a refrigerator at three degrees Celsius. If there was visible condensation on the agar plate‚ they were flipped to avoid the contact of heat that drove the moisture out of the agar dishes. The contents of the broth culture of E. coli was gently swirled
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Lab Report Microbiology Introduction Bacteria can be found almost anywhere. For human life‚ some help us‚ some hurt us‚ and others are neutral. It is now known that good bacteria‚ or normal microflora‚ can reach 1014 microbial cells. This is far more than the 1013 cells that make up the human body (Tannock‚ 1995). The total number of bacteria on Earth is estimated to be around 4-6 x 1030 (Horner-Devine‚ 2004). It is important to know the extent of bacteria‚ how they live‚ and how they are
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BHS002-1 Microbiology Practical Manipulation of bacteria – Part 1 Week 45 Since the early days of microbiology in the 19th century‚ culture on agar plates has been a central technique for the study of bacteria. This practical is designed to introduce students to the basic techniques required to manipulate bacteria. Students will gain experience with the streak plate procedure‚ used to isolate pure colonies of bacteria‚ and viable plate count methods. The latter involves serial dilution and spread
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Identification of Unknown # 15 Abstract. One of the most fundamental differential staining techniques used in the study of bacteriology is gram staining. There are two main types of bacteria‚ gram negative and gram-positive. The purpose of this experiment was to perform a variety of tests to identify the bacteria contained in the unknown sample labeled number 15. The following are the tests that were used to identify the two different bacteria. The
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rate similar to health care personnel. Microbiology students at the commencement of microbiology studies would be expected to have a carriage rate similar to the general population‚ therefore worth investigating whether there is evidence that the carriage rate of microbiology students is similar to hospital or age care personnel. (MLTM) The aim of this study is to determine the carriage rate of Staphylococcus aureus in the nares of second year microbiology students at RMIT University. Materials
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Staphylococcus species (Bergey). Ex 2-2 Colony Morphology showed circular white opaque colonies with smooth‚ entire margins when grown on TSA. This is key in helping to determine whether the EI was a Staphylococcus spp or a Mircroccus spp since Mircroccus spp. commonly show yellow color colonies when grown on TSA and Staphylococcus spp are commonly opaque white colonies when grown on TSA (Bergey). Additionally‚ Ex 3-10 Endospore Stain showed the EI to be non-endospore forming‚ which corresponds to the characteristics
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Marcescens culture. Though there was anexperimental error‚ the effect of UV light radiation on bacterial cells was observed. Comparingboth plates from another group‚ it was noted that though both plates were subjected to the sameUV light exposure‚ the M. luteus experienced more growth; this is because M. luteus has a higherlevel of resistance to UV radiation than S. marcescens. The S. marcescens colony was expectedto turn
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testing tubes and inoculated an EMB plate. This test would confir that coliforms were present. After letting the EMB plate incubate for 24 hours‚ if the agar contained a metallic sheen‚ then E.coli was present. To test the coliform‚ I then selected a colony from the agar and inoculated it onto a TSA slant and in a lactose broth. With the TSA slant now being incubated for 24 hours‚ I then performed a gram stain from it and record my results. From the TSA plates I also inoculated one tube of tryptone
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Survey of Nasal Carriage of Staphylococcus aureus in Microbiology 1 Students at RMIT Aim: To determine the carriage rate of Staphylococcus aureus in the nares of students taking second year microbiology courses at RMIT. Introduction: Carriage of S.aureus is important in hospital patients‚ preoperative patients‚ hospital staff‚ food handlers etc. because it carriage of S.aureus appears to play a key role in the epidemiology and pathogenesis of infection. S.aureus can cause localized and invasive
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|COLOR BEFORE: PURPLE |NO CHANGED ‚NO GROWTH | | |BLACK | | |TSA |COLOR BEFORE: WHITE |GROWTH | |MSA |COLOR BEFORE:RED LIGHT |GROWTH/NON-FERMENTATION
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