of E. coli would protect the bacteria from any antimicrobial properties of cilantro. A drop of cilantro juice and water in varying concentrations (1:10‚ 1:20‚ 1:40‚ 1:80) was added to a nutrient agar plate inoculated with S. epidermis and a nutrient agar plate inoculated with E. coli. The plates were incubated for 48 hours and then observed for a zone of clearing where the cilantro juice drop was placed. Cilantro was found to not display antimicrobial activity against either bacterium in the experiment
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NTRODUCTION Often in the study of microbiology‚ the need to identify an unknown microbe may arise. This aids in the recognition of which microbes are harmful or pathogenic as well as those that may be beneficial to us in some way. This report will detail how a similar project was performed in order to identify two unknown micobes using the techniques that were learnt during the course of the semester. We embarked on the project with the knowledge that we would have to identify a Gram(+) as well
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zone of inhibition of bacterial growth around each well is measured and the susceptibility is determined. Medium-Muller Hinton Agar (3.8gm/100ml of distilled water) was prepared‚ autoclaved at 121⁰ C for 15minutes at 15lbs and poured in sterile petri plates up to a uniform thickness of approximately 5-6mm and the agar was allowed to set at ambient temperature and used. Inoculums-The microorganisms were inoculated in Nutrient broth and incubated at 37⁰ C and were used as inoculums. 25 µl of inoculum was
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B.Sc. First Year Semester – I Paper I .Fundamentals of Microbiology Credit - 1 1 Scope & relevance of Microbiology i) ii) iii) Definition & concepts Types of microorganism Distribution of microorganisms in nature 2. Development of Microbiology as a Scientific Discipline 1.1 History i) ii) iii) iv) v) vi) Credit – 2 Early observation of microorganisms Spontaneous generation conflict : Contribution of scientists Recognition of the microbial role in diseases. Koch’s postulates Recognition of microbial
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iLab #2 Carbohydrate Tests INTRODUCTION - For this experiment we will be testing four different bacteria with four different tests‚ using glucose‚ lactose‚ and sucrose. Hopefully we will use the information from those test to be able to identify the organisms in each of the samples from the case studies. We will use the results from the four different tests along with the information of how different bacteria react to match up to the case
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References: Gerard J. Tortora‚ Berdell R. Funke‚ Christine L. Case (2010). Microbiology: an introduction (10th ed.). San Francisco‚ CA: Pearson/ Benjamin Cummings. James G. Cappucino‚ Natalie Sherman (2011). Microbiology: a laboratory manual (9th ed.). San Francisco: Benjamin Cummings. Micrococcus luteus. (n.d.). Retrived Jun 11‚ 2012 from the Wikipedia: the free encyclopedia: http://en.wikipedia
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Basingstoke‚ UK) and the targeted areas on the cutting board‚ tray‚ plate and wok were swabbed. Sampling has been done by swabbing all of these surfaces horizontally‚ vertically and diagonally by using standard templates with the size of 10 cm x 10 cm. The whole procedure will be carried out aseptically to minimize the risk of contamination. After swabbing‚ samples were put back into their respective
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Techniques for Isolation of Pure Cultures Objective : i. To perform the spread plate and the streak plate inoculation procedure for the separation of the cells of a mixed culture so that discrete colonies can be isolated. ii. To prepare a stock culture of an organism using isolates from the mixed cultures prepared on the agar streak-plate and/or the spread plate. Introduction : In order to be able to adequately study and characterize a certain microorganism‚ microbiologists need to separate
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GRAM STAIN THEORY: The most widely used staining procedure in microbiology is the Gram stain‚ discovered by the Danish scientist and physician Hans Christian Joachim Gram in 1884. Gram staining is a differential staining technique that differentiates bacteria into two groups: gram-positives and gram-negatives. The procedure is based on the ability of microorganisms to retain color of the stains used during the gram
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Journal of Microbiology‚ December 2012. ISSN: 2074-5346 Vol. 2‚ Issue 1 Assessment of pollution caused by tannery-waste and its impact on aquatic bacterial community in Hajaribag‚ Dhaka Mahbubul Hasan Siddiqee‚1‡ Md. Saiful Islam2 and M. Majibur Rahman3 assay 1 Microbiology Program‚ Department of Mathematics and Natural Sciences‚ BRAC University‚ Dhaka-1212‚ Bangladesh; Department of Microbiology‚ Stamford University Bangladesh‚ Dhaka-1217‚ Bangladesh; 3Department of Microbiology‚ University
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