NA transformation of E. coli: The two plasmids were added to individual tubes containing E. coli and one with no plasmids. The three samples of E. coli were heated in a 42°C water bath for 90 seconds to heat shock the bacteria so that the plasmids would be taken up by the E. coli. These samples were then incubated at 30°C for half an hour and then plated on LB agar. Each tube was plated on an LB plate and a LB + ampicillin plate. Ampicillin is an antibiotic that is effective against E. coli‚ both
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Drinking Age In other countries around the world if you are under the age of 21 you can drink alcohol some countries don ’t even have an age limit‚ but in America you have to be 21 to buy alcohol. You can go to war and die for your country when you are 18‚ you can also vote‚ buy cigarettes‚ but you can ’t buy alcohol. One of the main causes of the drinking age being set to 21 was teenagers were getting into car accidents after getting drunk. I am not saying driving under the influence should be legal
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Question One (25 marks) Based on module 7 – Budgeting Retail outlets purchase snowboards from Slopes Ltd.‚ throughout the year. However‚ in anticipation of late summer and early autumn purchases‚ outlets ramp up inventories from January through May. Outlets are billed when boards are ordered. Invoices are payable within 60 days. From past experience‚ Slopes’ accountant projects 20% of invoices are paid in the month invoiced‚ 50% are paid in the following month‚ and 30% of invoices are paid two
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things do go over time‚ for example‚ a band is running 30 minutes late so therefore the concert is running 30 minutes late so therefore the event manager may need to consider different solutions to the problem. These solutions could be for example‚ moving another band in their time slot. Problem solving is an essential skill needed as many problems may occur before or after the event and it is the event’s organisers role to help resolve of reduce these issues. Sometimes when planning an event‚
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Containerized 200 kW NAS Battery (to be released in 2015) 1. A “Plug & Play” 200 kW NAS Battery Unit (NBU) is under development at NGK. It will: ‐ Expedite shipping‚ installing and relocating multi‐MW NAS installations ‐ Accommodate a broad range of ambient site conditions: e.g. Operational temperature range is from ‐45 to 55C (special type) or –10 to 45C (standard type). Special type for salt‐air coastal environments is available. 2. A new 36 kW NAS module design is required for 200 kW NBUs
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Year 0 2007 Investment Capital outley Δ Net Working Capital Cash outley NWC Investment Recovery Equipment Salvage NWC (full recovery) Sales Cost of Goods Sold (-) SG&A Expense (-) Opperating Savings (+) Depreciation (-) Operating Profit before tax (16‚000‚000) Year 1 2008 (2‚000‚000) 400‚000 (400‚000) Year 2 2009 Year 3 2010 1‚000‚000 (600‚000) 1‚000‚000 4‚000‚000 3‚000‚000 200‚000 2‚000‚000 3‚000‚000 (200‚000) 10‚000‚000 7‚500‚000 500‚000 3‚500‚000 3‚000‚000 2‚500‚000 10
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1. Assuming that the distribution is normal for weight relative to the ideal and 99% of the male participants scored between (-53.68‚64.64)‚ Where did 95% of the values for weight relative to the ideal lie? Round your answer to two decimal places. X =5.48‚ SD = 22.93 5.48 – 1.96(22.93) = AND 5.48 + 1.96(22.93) = 5.48 – 44.9428 = AND 5.48 + 44.9428 = -39.4628 AND 50.422 (-39.46‚ 50.42) 2. Which of the following values from Table 1 tells us about variability of the scores in a distribution
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[pic] 1. Introduction 1. Name of the company NAS LAUNDRY 2. Nature of business NAS Laundry is a public company that focuses on a public service‚ laundry 3. Industry profile The main idea for developing this business is to provide laundry services for working people. Since working people will be home at home at night‚ they do not have enough time to wash their own cloth. So‚ they will prefer
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Maggie May‚ written by Rod Stewart‚ is a classic rock song that tells the story of a young man who was convinced to skip town with the girl of his dreams named Maggie. Now that he’s left‚ he realized what he is missing out on back home and he blames her for making him leave but he loves her too much to abandon her. This story is told through the different music conventions that help tell the story‚ not through words‚ but through sound. For example‚ the form of the song is split up into four repeating
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shopping behavior | FREE-FLOW LAYOUT | Incread creativity in decor items to display?sed impulse buyingVisual appealFlexibility | Possible confusionWaste of floor spaceDifficulty in cleaning | 2. What psychological effects of a pharmacy layout may influence customer-buying behavior? * Value/Fashion image – Presentation of the product influences image customer has regarding that product * Angles and sightlines – Customers view product at 45-degree angle and at eye level. Where a product
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