NA transformation of E. coli: The two plasmids were added to individual tubes containing E. coli and one with no plasmids. The three samples of E. coli were heated in a 42°C water bath for 90 seconds to heat shock the bacteria so that the plasmids would be taken up by the E. coli. These samples were then incubated at 30°C for half an hour and then plated on LB agar. Each tube was plated on an LB plate and a LB + ampicillin plate. Ampicillin is an antibiotic that is effective against E. coli‚ both
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Containerized 200 kW NAS Battery (to be released in 2015) 1. A “Plug & Play” 200 kW NAS Battery Unit (NBU) is under development at NGK. It will: ‐ Expedite shipping‚ installing and relocating multi‐MW NAS installations ‐ Accommodate a broad range of ambient site conditions: e.g. Operational temperature range is from ‐45 to 55C (special type) or –10 to 45C (standard type). Special type for salt‐air coastal environments is available. 2. A new 36 kW NAS module design is required for 200 kW NBUs
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"Narcotics Anonymous is a nonprofit Fellowship or society of men and women for whom drugs had become a major problem. We are recovering addicts who meet regularly to help each other stay clean." After that‚ they directed the attention to a table with the NA book and other books and pamphlets which could be purchased or are for free. After the text was read‚ people celebrated special occasions. New members were welcomed greatly‚ as we had one at the time‚ and also a mother and son who came in later. All
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Oisín i dTír na nÓg – Nótaí. Téamaí an tSleachta: Baineann an sliocht seo leis an óige agus aois. Ag tús an tsleachta‚ tá Oisín óg‚ láidir agus dathúil. Ach cailleann sé a óige agus déantar seanfhear dall de. Is téama eile é cráifeacht agus an Chríostaíocht le linn Phádraig Naofa. Cuidíonn sé le Oisín agus múineann sé soiscéal Dé dó. Feicimid draíocht mar théama freisin. Tá an capall ábalta eitilt thar farraige go Tír na nÓg. Tá neart as cuimse ag Oisín nuair a chaitheann sé an leac
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Sports Hero Li Na One of my sports heroes is one of the best women tennis player in China‚ her name is Li Na. Li Na‚ Chinese women tennis athletes‚ she was born in Wuhan China in 1982‚ she starts to play tennis when she was 6‚ in 1999‚ she become to professional tennis players‚ at that time she played a lot of ITF challenger and got a lot of champion. In year of 2000 it was the first time ever see tennis in my life‚ my dad took me to a tennis game in Shanghai‚ it was POLO open‚ LI Na was playing against
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Outsourcing is increasingly a component of a company’s HR Transformation strategy‚ be it tactical or strategic. As the number of success stories and lessons learned increases‚ stakeholder resistance to outsourcing is decreasing. One by one the traditional barriers to payroll outsourcing are being eliminated‚ as suppliers are offering more flexible solutions including pay-as-you-go options‚ pre-configured platforms and better integration with other HR modules. Solutions exist today for smaller‚ dispersed
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[pic] 1. Introduction 1. Name of the company NAS LAUNDRY 2. Nature of business NAS Laundry is a public company that focuses on a public service‚ laundry 3. Industry profile The main idea for developing this business is to provide laundry services for working people. Since working people will be home at home at night‚ they do not have enough time to wash their own cloth. So‚ they will prefer
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entitled “Ta-Na-E-Ka‚” by Mary Whitebird tells us about Mary‚ an Indian Kaw girl who tried to avoid the Indians’ old tradition which was called as Ta-Na-E-Ka. The reason why I choose this story to analyze is because I am interested in the existence of the old tradition of Indian living people within the modern culture which is very contrast with the tradition itself. The tradition I am talking about is a tradition of the Kaws that children at a specific age-usually 11 years old-make "Ta-Na-E-Ka"‚ an
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also known as “Daing na bangus” was one of the famous foods in the Philippines and it is one of the delicious and nutritious foods loved by most Filipinos. Fish like bangus contains proteins‚ fats‚ vitamins‚ calcium and iodine that is needed by our body towards a good and a healthy lifestyle. Through the process of deboning milkfish and marinating with vinegar and garlic‚ the product Daing na bangus became known in the country. Dagupan City is one of the famous sources of daing na bangus particularly
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PER REPORTER: Shelly said Na’kiyah and her mother (Sierra) got into it last night which resulted in her mother kicking her out at 10:00 at night. Shelly said Na’kiyah left the house with nothing but the clothes on her back and was walking through their neighborhood. Shelly said according to Na’kiyah‚ one of the parents of another child who also attends New Hope School picked her up. Na’kiyah said the parent who picked her up name is Tameka Brookes and she is friends with her daughter. Na’kiyah said
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