NA transformation of E. coli: The two plasmids were added to individual tubes containing E. coli and one with no plasmids. The three samples of E. coli were heated in a 42°C water bath for 90 seconds to heat shock the bacteria so that the plasmids would be taken up by the E. coli. These samples were then incubated at 30°C for half an hour and then plated on LB agar. Each tube was plated on an LB plate and a LB + ampicillin plate. Ampicillin is an antibiotic that is effective against E. coli‚ both
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Exploring the Personality Structure in the 11 Languages of South Africa Jan Alewyn Nel‚1 Velichko H. Valchev‚2 Sebastiaan Rothmann‚1 Fons J. R. van de Vijver‚1‚2 Deon Meiring‚3 and Gideon P. de Bruin4 1 2 North-West University‚ South Africa Tilburg University‚ The Netherlands 3 University of Pretoria‚ South Africa 4 University of Johannesburg‚ South Africa ABSTRACT The present study‚ part of the development of the South African Personality Inventory (SAPI)‚ explores the
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Containerized 200 kW NAS Battery (to be released in 2015) 1. A “Plug & Play” 200 kW NAS Battery Unit (NBU) is under development at NGK. It will: ‐ Expedite shipping‚ installing and relocating multi‐MW NAS installations ‐ Accommodate a broad range of ambient site conditions: e.g. Operational temperature range is from ‐45 to 55C (special type) or –10 to 45C (standard type). Special type for salt‐air coastal environments is available. 2. A new 36 kW NAS module design is required for 200 kW NBUs
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when not at home are less likely to have sex by age 16.” (Ikramullah‚ Manlove‚ Cui & Moore‚ 2009) In many situations it’s difficult for a parent to always be aware of what their child is doing. Although parents may want to be more involved with what their child does outside of home‚ it may be hard to do so with work schedules‚ younger siblings and just the everyday responsibilities of a parent. Sex Education Sex education is so readily available at many clinics and hospitals. Although this information
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Jose Rizal (1861-1896) Rizal was born on June 19‚ 1861‚ in Calamba‚ Laguna‚ the seventh child and the second son of the 11 children of Francisco Mercado and Teodora Alonso. Rizal’s parents were not only well-to-do‚ but also well educated‚ a rarity among Filipino families then. His father‚ a sugar planter and landholder‚ attended a Latin school in his native Binan town‚ also in Laguna‚ and a college in Manila. His mother‚ who had a good business sense managed some small enterprise‚ also studies at
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Maggie May‚ written by Rod Stewart‚ is a classic rock song that tells the story of a young man who was convinced to skip town with the girl of his dreams named Maggie. Now that he’s left‚ he realized what he is missing out on back home and he blames her for making him leave but he loves her too much to abandon her. This story is told through the different music conventions that help tell the story‚ not through words‚ but through sound. For example‚ the form of the song is split up into four repeating
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SAAS MARKETING FOR CEOS HOW TO GO TO THE TOP OF THE MARKET and stay there TA BLE OF CO N T E N T S i 1. Introduction 5. The Story of Shopify 8. Going to Market 9. Know Your Customer 10. Create a Product Your Customers Will Love 11. Create a Company Your Employees Will Love 12. Support Your Customer at All Costs 13. Create a Great Customer Experience 13. Marketing Takeaways 14. Getting to the Top of the Market 16. Early Days - Growth Hacking 17. Inbound Marketing (Content‚ SEO and Social Media)
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"Narcotics Anonymous is a nonprofit Fellowship or society of men and women for whom drugs had become a major problem. We are recovering addicts who meet regularly to help each other stay clean." After that‚ they directed the attention to a table with the NA book and other books and pamphlets which could be purchased or are for free. After the text was read‚ people celebrated special occasions. New members were welcomed greatly‚ as we had one at the time‚ and also a mother and son who came in later. All
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enough to establish friendly relationship between studio audience and the hosts. For me the program Showtime is less popular than Eat Bulaga (they were competition). They are alot of reasons; maybe EB is older than Showtime so it is more popular or may maybe the approach. Showtime is purely entertainment they give money but for fun not for the sake of public service unlike EB‚ there is what they called “Juan for all‚ All for Juan‚” a public service but in entertainment way. My point is they are targeting
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"MEMORIES" Nothing compares to having a family‚ a complete‚ happy family. I am proud to have had one. Unforgettable memories of a sweet childhood‚ growing up with pure love and affection‚ so many people wanted to have. I was nourished with hope‚ that someday I’ll have something more than I’ve expected. My parents gave me everything. They wanted me to have the best of endless possibilities. I owe my life to them. I am nothing without them. They are the reason why I have a fighting spirit‚ to finish
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