NA transformation of E. coli: The two plasmids were added to individual tubes containing E. coli and one with no plasmids. The three samples of E. coli were heated in a 42°C water bath for 90 seconds to heat shock the bacteria so that the plasmids would be taken up by the E. coli. These samples were then incubated at 30°C for half an hour and then plated on LB agar. Each tube was plated on an LB plate and a LB + ampicillin plate. Ampicillin is an antibiotic that is effective against E. coli‚ both
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links to two of the prime areas and one of the specific areas: Prime area: Physical development – one of the reasons why hopscotch links to physical development includes that the child has to leap in the activity. As the game goes on‚ the children may have to leap over two or more spaces at one time. Also‚ hopscotch requires a one-footed leap‚ and
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when not at home are less likely to have sex by age 16.” (Ikramullah‚ Manlove‚ Cui & Moore‚ 2009) In many situations it’s difficult for a parent to always be aware of what their child is doing. Although parents may want to be more involved with what their child does outside of home‚ it may be hard to do so with work schedules‚ younger siblings and just the everyday responsibilities of a parent. Sex Education Sex education is so readily available at many clinics and hospitals. Although this information
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Minal Patel is my distant cousin and she graduated from University of Texas at Austin on 2013. She majored in pharmaceutical science and earned a master’s degree. She is currently a staff pharmacist who dispenses medications and evaluates all aspect of medication use. In the 30 minutes interview‚ I received thoughtful insights on how I can improve my stages of college life. We talked in depth about what I can do differently that can make my college life enjoyable and less stressful. We discuss how
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Organized religion is the way most people live their lives by whether it be spiritual‚ believing in a high power‚ or a god. It can promote inner peace‚ unity‚ and harmony when it has a positive effect on society; however‚ It can be destructive‚ harmful‚ and evil when it has a negative effect. One negative effect of organized religion was the Holocaust. It is a past world event that brought a large amount of pain and suffering to those involved and many years later. The Germans believed
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ng mga bagay na gustong gusto mong gawin ay tinatawag na GAWI‚at ang magagandang gawi na ito ay madalas na ginagawa at ito ay nakaaloob sa moral na batas.Ito ay nagiging BIRTUD.ang birtudna nakaaloob dito ang siyang kinakailangan uang maisagawa ang isang gawain na kaakibat ng resonsabilidad. IN: –preposition 1. (used to indicate inclusion within space‚ a place‚ or limits): walking in the park. 2. (used to indicate inclusion within something
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Abstract Art‚” referenced previously.) • The image can only contain the colors red‚ yellow‚ blue‚ black‚ white‚ and gray. • The image can only contain rectangular shaped planes and lines. • If two lines run in the same plane‚ they are parallel. • Lines may only intersect at 90o angles. • The ratio of white space to color (red‚ blue‚ and yellow) should be 2:1. • Areas of complexity and simplicity must be placed in opposite corners. • No Mondrian art my contain symmetry. 1. Use a graphics program (e.g
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ASSIGNMENT BRIEF Qualification Unit number‚ Code and Title Pearson BTEC Level 5 HND Diploma Business Unit 34‚ R/505/8181‚ Operations Management in Business Module Leader: Lecturers: Abimbola Taiwo Ousman Kolley‚ Abimbola Taiwo Distribution date Submission deadline W/C – 22/09/14 7th December 2014 Assignment title Operations Management in Business Learning Outcome Learning outcome Assessment Criteria In this assessment you will have the opportunity to present evidence that shows you are able
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Many works have been devoted to the differences in communication between people. It has been proved that such factors as the interlocutors’ education or the status of the relationship influence people’s utterances. Furthermore‚ linguists claim that also biological sex influences the choice of language people use to communicate with each other. In discourse on different ways of communication of males and females‚ linguists have resigned with the term “sex” replacing it by the “gender”
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The Bible (from Koine Greek τὰ βιβλία‚ tà biblía‚ "the books") is acanonical collection of texts considered sacred in Judaism orChristianity. Different religious groups include different books within their canons‚ in different orders‚ and sometimes divide or combine books‚ or incorporate additional material into canonical books. Christian Bibles range from the sixty-six books of the Protestant canon to the eighty-one books of the Ethiopian Orthodox Church canon. The Hebrew Bible‚ or Tanakh‚ contains
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