Description: A peroxidase enzyme‚ which was extracted from a brassica compestris (turnip)‚ is tested under various conditions in temperature‚ pH level‚ and competitive inhibitor (hydroxylamine). ABSTRACT: In order to determine the properties of an enzyme‚ a peroxidase enzyme was extracted from a brassica compestris (turnip) and tested under various temperatures‚ pH levels‚ and by a competitive inhibitor (hydroxylamine). The enzyme activity was measured in various ways depending on the activity
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Determining the Catalytic Properties of the Enzyme Peroxidase Extracted from a Turnip Under the Conditions of Temperature‚ pH‚ Boiling and Competitive Inhibitors By Robin Caserta BIO 101 September 30‚ 2013 ABSTRACT The enzyme‚ peroxidase‚ extracted from a turnip was tested for its efficiency in binding to its substrate and its stability under several conditions. To do this‚ we tested effects on peroxidase activity‚ first‚ with different amounts of the enzyme‚ next at temperatures of
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The Affects of pH on Peroxidase Enzyme Claire Bell Biology 1130 Jazmen Myers Section 36 Abstract Peroxidase is a turnip enzyme; it is used in the oxidation of hydrogen peroxide to lower activation energy‚ speeding up the reaction. The activity of peroxidase is highly dependent on its environment and most importantly the pH level. Peroxidase has been the focus of many recent studies and is believed to possibly reduce swelling among other things. We conducted an experiment testing
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Horseradish Peroxidase Abstract There are several factors that can affect the rate of reaction of peroxidase such as temperature‚ pH‚ concentration of peroxidase present and whether or not it has been boiled. Our experimental data demonstrated that peroxidase activity peaked between 23 degrees Celsius and 32 degrees Celsius. We found the pH to be 7 for optimal activity. As far as the concentration is concerned our results showed that as the concentration of horseradish peroxidase doubled so
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1. Introduction Plumbago zeylanica L. belonging to the family Plumbaginaceae is an important plant of medicinal value. In the Arabian Peninsula‚ it is mainly distributed over Oman‚ Yemen and the Southwestern region of Saudi Arabia. The use of the roots of P. zeylanica for treating skin problems has been recorded by early Muslim physicians. P. zeylanica is greatly valued in Ayurveda for treatment of cough‚ asthma and gastrointestinal disorders. In Sushrutha Samhitha it has been described as antiseptic
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NaCl Effects on Peroxidase Activity My experiment was to see if adding NaCl to solution would have any effects on peroxidase activity. The materials that were used in this experiment were pH 7 buffer(DI water)‚ peroxidase‚ NaCl‚ guaiacol and hydrogen peroxide; added in that order. Blanks were created for each NaCl concentration‚ 0%‚ 5%‚ 7.5% and 10%. Each cuvette had .5ml of pH 7 buffer‚ 1ml of peroxidase‚ .02ml guaiacol for the experimental cuvettes and 0ml of guaiacol for the blank cuvettes‚
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Enzymes speed up chemical reactions and at the active site‚ a substrate can be broken down or two substrates can form a larger molecule. Hydrogen peroxide is broken down by peroxidase into water and oxygen which is released in the form of gas bubbles. If there is a large amount of bubbles‚ it means there is a large quantity of peroxidase breaking down the hydrogen peroxide. Denatured enzymes are enzymes whose shape has been altered and are no longer active (Wiggins). Ethanol is able to denature enzymes
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Healthy Lifestyle Top of Form 1 Corinthians 6:19-20 Or do you not know that your body is a temple of the Holy Spirit within you‚ whom you have from God? You are not your own‚ for you were bought with a price. So glorify God in your body. 1 Corinthians 10:31 So‚ whether you eat or drink‚ or whatever you do‚ do all to the glory of God. 1 Corinthians 3:17 If anyone destroys God’s temple‚ God will destroy him. For God’s temple is holy‚ and you are that temple. Romans 12:1-2 I appeal to you therefore
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The Turnip-snedder The poem “The Turnip-Snedder” by Seamus Heaney is about an archaic machine that was used in traditional farming‚ to cut the heads off turnips. The turnip-snedder is personified and portrayed in multiple ways. It is personified in a monstrous way but also in a very god like and powerful manner. The turnip snedder is also used to reflect the idea how some people refuse change even though it is inevitable. The poet’s attitude is nostalgic with a sinister undertone of violence and
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results that higher concentration of iron cofactors shows the higher absorbance. The importance of the existence of iron in this reaction is that the peroxidases in plant cells respond to environmental stress by‚ in this case‚ catalyzing the conversion of toxic metabolic wastes‚ H2O2‚ into the harmless products‚ H2O and O2‚ faster. Therefore‚ as peroxidases help minimize and eliminate harmful chemicals‚ the iron cofactors that help peroxidase’s activity raise the possibility of plants’ survival in harsh
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