of peroxide Time (min) Qualitative color 1:00 5 2:00 6 3:00 7 4:00 8 5:00 9 Table 2: effect of increased pH on peroxidase Increased pH by 5mL Color intensity 2 10 5 10 1 10 8 9 11 10 3 10 Time in minutes Graph: Graph1: The effect of pH on the enzyme Peroxidase Y- value rate of reaction X- value = pH of solution Conclusion: This experiment was to test if increased amount of pH
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hydrogen peroxide at 240 nm according to Aebi [25]. Superoxide dismutase (SOD) activity was assessed by pyrogallol autoxidation according to Marklund [26]. Reduced glutathione (GSH) concentration was measured according to Ellman [27]. Glutathione peroxidase (GSH-Px) activity was determined in accordance to procedure described by Beutler [28]. Isolation of mitochondrial fraction from kidney tissues Briefly‚ a portion of the fresh kidney tissue was homogenized in isolation buffer [250 mM Sucrose‚ 1
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at an appropriate dilution and incubated with the membrane. Antibodies present in the serum bind to the protein(s). In order to detect the bound antibodies‚ anti-immunoglobulin antibodies coupled to an enzyme alkaline phosphatase or horseradish peroxidase are added. This anti-IgG enzyme is commonly called a "second antibody" or "conjugate". Finally‚ after excess second antibody is washed free of the blot‚ a substrate is added which will precipitate upon reaction with the conjugate resulting in a
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Brassica rapa var rapa (Man Jing Turnip) is an important biennial‚ outcrossing‚ mesopolyploid herbaceous plant in the family Brassicaceae‚ native throughout Europe‚ Russia‚ Central Asia and the Near East (Prakash & Hinata 1980 ) with Europe proposed as one centre of origin.. In the alpine regions of the Qinghai-Tibetan Plateau it was widely used as a food source‚ when other crop production was limited approximately 2000 B.C. These highlands range in altitude between 3000-4000 m and are characterized
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Oxygen Requirements of Bacteria BACKGROUND The GasPak system is useful for culturing anaerobic bacteria on standard microbiological media because the GasPak generates carbon dioxide and hydrogen. The hydrogen will combine with oxygen present in an anaerobic jar to produce water. This system can reproducibly attain oxygen levels in the parts per million range if used correctly. This is the best method for determining the oxygen requirements of unknown organisms. A candle jar is useful
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Introduction : Solanum nigrum Linn. commonly known as Black Nightshade is a dicot weed in the Solanaceae family. Other names of S. nigrum are European Black Nightshade or just black nightshade‚ Duscle‚ Garden Nightshade‚ Hound’s Berry‚ Petty Morel‚ Wonder Berry‚ Small-fruited black nightshade. It is a species of the Solanum genus‚ native to Eurasia and introduced in the Americas‚ Australia‚ Asia and South Africa. The plant is cultivated as a food crop‚ both for its fruit and its leaves in some
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“Their accounts conflict on significant details. But one thing they all agree on: the event provoked a seismic response” (Eksteins 10). Similarly‚ there are many accounts of what happened during The Great War‚ however‚ there is no accurate description of soldiers’ experiences. There are many resemblances between the opening night of Le Sacre du printemps and The Great War‚ but the resemblance that stands out the most is the different experiences each spectator had from both of these events. In “All
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take care of the children‚ made the turnips from her “victory Garden” so they could save money and not buy canned goods. Knowing she would get in trouble for not eating the turnips‚ Molly abandoned her plate till her mother came home and saw what she left behind. Being the supporter of the family‚ Mrs. McIntire Knew that times were hard‚ and turnips were not the tastiest thing in the world. So with a little sugar and words of wisdom‚ Mrs. McIntire turned the turnips into something delicious. With one
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peroxide is a highly active chemical used in household products for bleaches and cleansing wounds. In a cell its build up would be highly toxic. However‚ liver cells contain an enzyme‚ catalase‚ which immediately breaks down hydrogen peroxide. It is a peroxidase and breaks up the toxic hydrogen peroxide to water and oxygen which are both nontoxic. The reaction is exothermic‚ meaning that energy is released in the form of heat. It is the fastest known enzyme. Hydrogen peroxide Water + Oxygen 2H2O2 2H2O
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Lesters house carrying a bag of turnips. He decides to stop and talk to Jeeter for some advice on
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