IB SL Biology Lab Molecular Biology: Transformation and Electrophoresis Christina Qi 2/16/07 Aim: How can a plasmid be engineered to include a foreign piece of DNA and how does gel electrophoresis separate DNA molecules present in a mixture? Hypothesis: If the pGLO plasmid is inserted into competent Escherichia coli cells‚ then the transformed bacteria will be resistant to ampicillin and will glow green under UV light. If samples of DNA are cut using certain restriction
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The cell theory‚ or cell doctrine‚ states that all organisms are composed of similar units of organization‚ called cells. The concept was formally articulated in 1839 by Schleiden & Schwann and has remained as the foundation of modern biology. The idea predates other great paradigms of biology including Darwin’s theory of evolution (1859)‚ Mendel’s laws of inheritance (1865)‚ and the establishment of comparative biochemistry (1940). First Cells Seen in Cork While the invention of the
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UMUC Haircuts is a small business owned and operated by Myra Morningstar. In light of recent new businesses coming into her market she has plans to expand her business into the adjacent building and has found minor flaws in her business that she feels could hold her business back. Myra is seeking a Cost Leadership Strategy that will incorporate ideas of keeping the end service price low‚ to keep her customers returning. To keep the price low she must keep the cost of doing business low as well‚ to
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Lab 10 Name: Complete the following then save with your name and attach to the dropbox for lab 10. Data Tables and Post-Lab Assessment Experiment 1 Post-Lab Questions 1. Label each of the arrows in the following slide image: A. Chromosomes B. Nucleus C. Cytoplasm D. Cell wall 2. What is the difference between the rough and smooth endoplasmic reticulum? 3. Would an animal cell be able to survive without mitochondria? Why or why not? 4. What
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Name: Angel Simon Pre–Lab Question Ocular lens 1. Label the following microscope using the components described within the Introduction. Experiment 1: Virtual Magnification Exercise Post-Lab Questions 1. At what magnification do you first notice the ragweed pollen? Answer: 1000 2. Which is bigger‚ a rhinovirus or E. Coli? Answer: E. Coli 3. Based on the magnification‚ how many of the E. Coli can fit into the same space as the
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Biology- cell organelles assignment Animal cells: Introduction: An animal cell is called a eukaryotic cell; it has a cell membrane‚ cytoplasm and a nucleus. The cell surface is covered by a membrane only. The cell membrane is strong and keeps the cell together even though it is thin and flexible. The cell membrane also controls what goes in and out of the cell e.g. nutrients go in and waste comes out. The cytoplasm is inside the nucleus and it controls the cells activities. An animal is
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Some of you have been asking for clarification regarding your optional use of the MSAF‚ the student self-reporting absence form. As you may know‚ this is allowance that you are permitted to use once per term to address temporary illnesses and deadline pressures. The allowance provided is generally five days‚ but faculty are asked to specify how the MSAF is to be applied in their particular course. Here are my guidelines: For all other more serious illnesses and absences‚ you would follow the
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UMUC Frederick MD Network Proposal The network IP addressing scheme for the Frederick MD building should be designed to ease network management. A well thought out network subnet design enables administrators to quickly locate computers by IP address by building floor and room‚ and whether the computer is a server‚ a student computer‚ staff computer or instructor’s computer. A proper subnet design will also minimize broadcast traffic that decreases network performance‚ by reducing
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LAB 4 Read over the parts of the microscope and answer the following questions: 1. What do you call the lens you look through on the microscope? Ocular 2. What is the difference between the ocular lens and the objective lens? Ocular lens is the lens you look through and objective lens is the lens that is close to the stage. 3. Where do you place the slide on the microscope? the stage under the stage clips 4. Which adjustment‚ course or fine‚ do you use when you are observing the
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Instrumental methods of Biochemical analysis ..................... 1 1. Flame Photometry ................................................................... 1 2. Fluorimetry ........................................................................... 10 3. Nephelometry ....................................................................... 12 4. Basic principles and applications of Potentiometry .................. 16 II. Separation techniques ..........................................................
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