Genetic transformation of Escherichia coli with pGLO (Adapted from: Biotechnology Explorer: Bacterial Transformation: The pGLO System. Instructors Guide. BIO-RAD). Objectives a. To understand one of the most commonly used techniques for introducing DNA into E. coli cells and its use in molecular cloning. b. To become familiar with the concept of using green fluorescent protein (GFP) as a molecular tag for studying gene expression in bacteria and other organisms.
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Tim Readmond AP Biology Lab Report I. Title a. Modeling Diffusion and Osmosis II. Introduction a. If one places a 1.0 M solution of glucose inside a bag and then places that bag into a beaker containing a 1.0 M solution of sucrose‚ the percent of mass lost in the bag is 10.5%. The solution in the bag is hypertonic while the solution in the beaker is hypertonic‚ which is why water moves from the bag to the beaker and the bag loses mass. b. The purpose of this experiment is to see whether
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Section______________________________ Virtual Lab: Enzyme Controlled Reactions Worksheet Go to the following website to complete the virtual lab: http://www.mhhe.com/biosci/genbio/virtual_labs_2K8/pages/EnzymeControlledReactions.html Due: Wednesday‚ March 28‚ 2012 during your respective lab time 1. Which of the following does NOT apply to an enzyme: a. Catalyst b. Inorganic c. Protein d. All of the above apply to an enzyme 2. When an enzyme catalyzes a reaction: a. Substrate(s)
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Flame lab test Chemistry Introduction The lab test performed was to determine characteristic colors that were produced by specific metallic ions that are shown in a flame. This happens when an electron gains energy; the electron moves from an energy level that’s farthest away and to an empty orbital close to the nucleus with higher levels‚ so one of the electrons gives off energy. A flame test is a visual test where the energy is in the form of a color change and the change can
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Histology Laszlo Vass‚ Ed.D. Version 42-0013-00-01 Lab Report Assistant This document is not meant to be a substitute for a formal laboratory report. The Lab Report Assistant is simply a summary of the experiment’s questions‚ diagrams if needed‚ and data tables that should be addressed in a formal lab report. The intent is to facilitate students’ writing of lab reports by providing this information in an editable
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xxx1 xxxxxx Ms. xxxx Biology 20 November 2013 Osmosis Lab Problem: What’s the order of the concentration for each solution and how does it affect the movement of water? Hypothesis: Experimenter thinks the order of the solutions according to their concentration is D‚A‚E‚C‚B‚ from smallest. And the water will pass through semipermeable tubing bag from low to high concentration to thin the concentration inside the bag. Osmosis is a diffusion of water particles and is one of the passive transport
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Investigation and Preparation of Esterification Lab Introduction Esters are classified as organic compounds commonly derived from carboxylic acids. They are compounded from the reaction of a carboxylic acid with an alcohol in the presence of a strong acid to be used as a catalyst. The formula that represents an ester is R-COO-R where both Rs’ are alkyl groups‚ one which is bonded to an oxygen‚ and the other to a carbon which is double bonded to 1 oxygen and single bonded to the other. Esters
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BMA 253 Intermediate Accounting Assignment #2 PART I Directions: 1) Complete the table below. 2) Calculate the estimated bad debts using aging of receivables method. Age Balance 1-60 days 61-120 days 121-180 days Over 180 days $80‚000 Probability Uncollectible of Default 2% $70‚000 4% $40‚000 8% $32‚000 11% Total PART II Directions: 1) Calculate the inventory cost for both periodic and perpetual method LIFO and FIFO using the following information and table below to fill in the shaded
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Abstract This lab was focused on determining the optimal temperature of the enzyme amylase responsible for catabolizing starch polymers and to see how different temperatures affected the rate as well as how effectively the enzyme worked. To proceed with the experiment the group set up four different test tubes for each‚ bacteria and fungal amylase‚ and labeled them accordingly with different temperatures as well as different solutions . Then the spot plates were placed on the time and temperature
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Measurements Lab Name Length Measurements – Follow the Instructions in the Lab Manual and fill in your data in the tables provided. Data Table 1 – Length measurements |Object |Length (cm) |Length (mm) |Length (m) | |CD or DVD |12.00 |120.0 |.1200 | |Key
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