Bacterial Smears Are Fixed before Staining to? Answer It is important to heat fix the bacterial smear before staining so as to‚ kill the bacteria‚ firmly adhere the smear on to the microscopic slide to prevent washing off during staining‚ and to allow the sample to readily take up the stain. Reference: www2.hendrix.edu What is the purpose of heat- fixing the smear? It helps the cells adhere to the slide so that they can be stained. The purpose of heat fixing is to kill the organisms without
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Identification of Unknown Carbohydrates using various biochemical tests Date of experiment: 11/10/2012 Name of Student: Vicki Marshall Introduction A series of biochemical tests was carried out to identify unknown carbohydrates. There were six carbohydrates that needed to be identified; they have been randomly labelled A-F. The carbohydrates are glucose‚ fructose‚ maltose‚ lactose‚ sucrose and starch. There was six tests that were carried out to help identify them‚ these were: Iodine Test
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A Report of Experiment 1 Purification and Identification of An Unknown Mixture Aim To separate a mixture of a neutral (ester‚ alcohol‚ ketone‚ aldehyde or ether) and an acidic compound (phenol or carboxylic acid) or a neutral and a basic compound (amine)‚ and to identify the unknown mixture by physical (M.P. or B.P.) and spectroscopic methods (NMR‚ IR and MS). Method The mixture (sample number: 27) was dissolved in 200 mL of CH2Cl2 and then extracted with 10% NaOH. The acid was removed
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“Fighting Invisible Killers” Scholastic Scope: The Language Arts Magazine January 2014 Edition‚ pages 5-9 Bacteria surround us every day. These little “bugs” are invisible to the eye and most do not harm us. Many are necessary for us to survive‚ like the bacteria in our stomachs and intestines that help us to digest food. But some bacteria are very dangerous to us. Addie Rereich became very sick in May 2011‚ when she was 11 years old. What started as something that her doctors and mother
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Bacteria are the most numerous type of microorganism found in the rhizosphere of the soil. They produce secondary metabolites which are capable of producing antibiotic which eventually inhibit or kill bacteria. The rhizosphere region of the soil is a highly favorable habitat for the proliferation‚ activity and metabolism of numerous microorganisms. The magnitude of this area depends on the plant and the size of the roots that the plant possesses. Bacteria are among the microorganisms living in the
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Bacterial Contamination April 15‚ 2013 Bacteria Contamination The definition of bacterial contamination is food contamination refers to foods that are spoiled or tainted because they either contain microorganisms‚ such as bacteria or parasites‚ or toxic substances that make them unfit for consumption This is very serious and people should take more precaution‚ food contamination is a serious because it results in foodborne diseases that each year affect an estimated seventy-six million
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Methodology By using aseptic‚ a little cultured bacteria was inoculated on the TSA agar. A quadric streak was making. Inoculation loop was heated and keep it cold for a while before the next quadratic streak. Six agar plates were observed for 24 hour at temperature of 30ºC. Choose one from the dense colony and make a sub-culture on the new agar plate. The step was repeated to get a single colony‚ which is pure colony. a) Sequestration of bacteria from fish organs Methodology Dissecting set
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MANIPULATION OF BACTERIA INTRODUCTION: In this experiment that we performed‚ there were many methods that were used to help us manipulate and identify the bacteria E.coli on a MacConkey agar plate. The first part of the experiment involved the methods of manipulating‚ identifying and counting the bacteria and the second part was to find out whether the bacteria E.coli was the only type found in the given area by gram staining. E.coli was the chosen bacteria for this type of experiment. It is
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UNKNOWN LAB REPORT UNKNOWN NUMBER 54 Ron Williams 7-28-05 Bio 205 Prof. Curlee Fall 2005 Purpose There are many reasons for knowing the identity of microorganisms. The reasons range from the knowing the causative agent of a disease in a patient‚ so as to know how it can be treated‚ to knowing the correct microorganism to be used for making certain foods or antibiotics. This study was done by applying all of the methods that have been learned so far in the microbiology laboratory
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safe‚ reliable and efficient water supply to satisfy the demand of all sectors of the economy. Whilst over the ensuing years there has been a steady increase in demand‚ there has not been the commensurate investment in infrastructure and the identification of alternative sources of supply‚ specifically targeting the industrial and agricultural sectors. WASA’s future operations must be viewed in the broader context of Government’s seven (7) pillars of sustainable development. Inherent in this is
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