become increasingly more important. The purpose of this study was to identify an unknown bacterium in a controlled laboratory environment over a 5 week period. Utilizing a variety of differential testing and staining methods learned in the microbiology course‚ students were to determine the identity of an assigned unknown organism. Observations were made and recorded each week to narrow down the scope of identification. Data has been presented in the tables‚ charts and drawings herein and reflect
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Report Unknown Bacteria Staining Gram Acid Fast Spore The first practical for microbiology was staining of an unknown bacteria. Professor Goode provided an unlabeled bacteria in which each individual were to conduct gram‚ acid fast‚ and spore staining tests from memory. When the gram staining test was conducted a positive results was obtained. When views on oil bacteria appeared purple and rod-like. I noted this result and looked at the lab key to see what the results narrowed my bacteria choices
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this experiment which are the brand of dishwashing detergent; Palmolive‚ Morning Fresh and Ultra Coles Brand and the type of bacteria used which are; Micrococcus Luteus and an unknown bacteria from a sponge. Dependent Variable The dependent variable is the factor that is being tested which is the size of the zone of inhibition from the detergent solution and the bacteria. It will be measured in millimetres using a ruler. Constant Variables Constant Variables are set in place in order to produce
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Unknown Bacteria Lab Report Introduction The purpose to this lab was to identify an unknown bacteria from a mixed culture provided to us by our instructor. This study was done by applying all of the methods that have been instructed on thus far in microbiology laboratory class. Each test performed‚ provided us with some key information about the unknown organism in question and how the bacteria function. Over a two week period‚ eight prepared types of test media were provided to identify
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MIC 230 Unknown Identification Report The objective of this experiment was to identify an organism from a mixture of two unknown bacterial species. In order to accomplish this‚ I first plated my unknown mixture on Tryptic Soy Agar (TSA)‚ Columbia Naladixic Acid (CNA)‚ and MacConkey’s Agar (MAC) plates. After 48 hours of incubation‚ it was unclear that two different bacterial colonies had grown on my TSA plate. Only one type of colony was evident. However‚ it was apparent that I had successfully
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then incubated at 37 degrees Celsius for 1½ hours. If negative then continue incubation up to 18 hours. * If positive (i.e.‚ the suspect colony is S. aureus)‚ the serum will coagulate‚ resulting in a clot (sometimes the clot is so pronounced that the liquid will completely solidify). * If negative‚ the plasma remains liquid. The negative result may be S. epidermidis but only a more detailed identification test can confirm this‚ using biochemical tests as in API tests and BBL CRYSTAL methods
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Identification of Unknown Organic Compound by Melting Point (M.P)‚ Boiling Point (B.P) and Infrared Spectroscopy Methods and Background The main objective of this lab is to identify the given unknown organic compounds with various methods. Identification of an unknown compound is important to perform through the process of melting point (M.P)‚ boiling point (B.P) and Infrared spectroscopy (I.R). Index of Hydrogen deficiency (IHD) and elemental analysis makes an experiment more efficient in
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Appendix B – Identifying Bacteria Bacteria identification is accomplished in a number of ways. Two common tools microbiologists use to identify unknown bacteria include dichotomous key and biochemical tests. The dichotomous key is useful when a microbiologist only needs to know which group an unknown microbe belongs to on a general level. When a microbiologist needs to identify a specific bacterium‚ biochemical tests are used. PART ONE: GENERAL BACTERIA IDENTIFICATION Review the dichotomous
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Identification of an Unknown Organic Acid Chem 1211K Lab Drawer #15 Wednesday November 13th‚ 2013 Unknown Number: 3334025-CF13 Table of Contents Pg. # Abstract 3 Experimental Report 4-7 Results and Discussion 7-12 Conclusion 13 **pKa Graph 14 Abstract Identifying this organic acid was an extensive task that involved several different experiments. Firstly‚ the melting
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Experiment H: Identification of an Unknown Organic Compound The objective of this lab was straightforward. We were given an unknown compound and we were to perform an IR spectroscopy and as well as NMR spectroscopy. With the IR spectroscopy‚ I was able to name the functional groups I have on my compound and further confirmed my assumptions by looking at the NMR spectroscopy after. The unknown number I was given was number 203. The molecular weight of the compound was 121. From the molecular weight
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