species of bacteria look similar under the microscope and also have the same staining results (ex. Gram stain). To be able to differentiate between the different species‚ one can look at the metabolic differences (fermentation)‚ as well as the environmental condition differences (temperature‚ pH‚ oxygen requirements). Being able to manipulate these conditions in a controlled environment can help to correctly identify the exact bacteria. Different media can be used to culture and identify bacteria. Some
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isolate and identify two unknown bacteria from a mixed culture provided to us by our instructor. This study was done by applying all of the methods that have been instructed on thus far in microbiology laboratory class. Each test performed‚ provided us with some key information about the unknown microbes in question and how the bacteria function. Materials and Methods Over a two week period‚ eight prepared types of test media were provided to identify the assigned unknown mixed cultures. Not
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The purpose of the unknown bacteria lab assignment was to select an unknown bacteria culture and‚ through a series of metabolic tests‚ identify which bacteria genus resided in the pure culture received. A nutrient broth inoculated with bacterial culture (numbered 45‚ henceforth referenced as U45) was selected and a streak plate was made to isolate a pure culture for use throughout the assignment. From the streak plate‚ several slides were made to determine the morphology of unknown 45. A Gram stain
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Identification of Two Unknown Species of Bacteria in Tube #72 Introduction: There are many reasons for knowing the identity of microorganisms. The reasons range from knowing the causative agent of a disease in a patient‚ so as to know how it can be treated‚ to knowing the correct microorganism to be used for making certain foods or antibiotics. This study was done by applying all of the methods that have been learned so far in the microbiology laboratory class for the identification of an unknown bacterium
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Identification and Diagnosis of Unknown Enteric Bacteria in an Infected Patient Using an Enteropluritube Brooke Addario 2/16/2015 BIO 488C - Section J TA – Jacob Karsten Introduction: Enteric bacteria are members of the Enterobacteriaceae family of microorganisms. These gram negative‚ rod shaped‚ facultative anaerobes are found in the intestinal tracts of humans and animals. Enteric bacteria can be pathogenic; however‚ they are also a normal part of human bacterial flora and are therefore
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Unknown Bacterium #22 Purpose The purpose of this experiment was to find the identity two unknown bacteria in the given test tube. Materials •Bacterial Loop •Bunsen Burner • Petri Dish with agar •Crystal Violet •Gram’s Iodine •95% Ethanol •Safrin •Glass Slides •Microscope •Unknown bacteria in test tube Procedure On the first day‚ one plate was streaked qualitatively and left it in the
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Report Unknown Bacteria Staining Gram Acid Fast Spore The first practical for microbiology was staining of an unknown bacteria. Professor Goode provided an unlabeled bacteria in which each individual were to conduct gram‚ acid fast‚ and spore staining tests from memory. When the gram staining test was conducted a positive results was obtained. When views on oil bacteria appeared purple and rod-like. I noted this result and looked at the lab key to see what the results narrowed my bacteria choices
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Unknown Bacteria Lab Report Introduction The purpose to this lab was to identify an unknown bacteria from a mixed culture provided to us by our instructor. This study was done by applying all of the methods that have been instructed on thus far in microbiology laboratory class. Each test performed‚ provided us with some key information about the unknown organism in question and how the bacteria function. Over a two week period‚ eight prepared types of test media were provided to identify
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this experiment which are the brand of dishwashing detergent; Palmolive‚ Morning Fresh and Ultra Coles Brand and the type of bacteria used which are; Micrococcus Luteus and an unknown bacteria from a sponge. Dependent Variable The dependent variable is the factor that is being tested which is the size of the zone of inhibition from the detergent solution and the bacteria. It will be measured in millimetres using a ruler. Constant Variables Constant Variables are set in place in order to produce
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Proteus mirabilis is a small gram-negative bacteria bacilli and a facultative anaerobe. A facultative anaerobe is when an organism can live in the absence or presence of oxygen. It is also a prokaryote‚ a single celled organism lacking a cell membrane bound nucleus‚ and can be divided into bacteria and archaea. Proteus mirabilis is characterized by their motility‚ its ability to distinguish maltose‚ and its inability to distinguish lactose. Proteus has the ability to stretch itself out and secrete
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