the semester‚ samples of bacteria were obtained from various sources. Two bacterial colonies were isolated from plates that were incubated in the lab and these bacteria became the basis for this project. The bacterial cultures were maintained and used for various physiological‚ cultural‚ and biochemical tests and observations over several weeks. The purpose of culturing bacteria over half the course of the semester was to learn various techniques used in microbiology labs and to subsequently utilize
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Requirements for Unknown Report You have been provided a mixed culture (a gram positive and a gram negative) from which you should have isolated each type of organism‚ described their colonies on a TSA plate‚ and described their cellular morphology. You were graded on this activity separately. After you have isolated your gram negative in pure culture you will be subjecting it to various biochemical tests and making observations about the cells‚ colonies‚ growth temperature preferences‚ etc. in
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spectrometry. We will do this by making 2‚ 4 a DNPH derivative and checking the melting points. Theory: By Using specific methods of compounds detection‚ we can match an unknown compound with a known compound because similar compounds will display similar characteristics. In this experiment‚ identifications of the unknown ketone was accomplished using thin layer chromatography‚ melting point‚ and NMR spectrometry. Thin layer chromatography is very quick but sensitive way of determining the components
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health and is easily infectious with contaminated sustenance. E. coli is gram-negative bacilli tested in clinical settings from stool specimens. The tests detect the presence of disease causing pathogens of E. coli that produce Shiga toxins. E. coli bacteria commonly occur in nature and they are a necessary component of the digestive process in humans and most other mammals. Most strains of E. coli are harmless‚ but pathogenic E. coli can be responsible for inflammation of the stomach and intestines
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experiment is to determine the unknown DNA plasmid using restriction enzymes and conducting electrophoresis finally comparing the resulting fragments with the known restriction map. In this lab‚ it succeeds in showing the fragments. In this report we will discuss the‚ results‚ limitations and possible errors. Introduction In biology restriction enzymes are used in several ways to modify and manipulate DNA molecules. One common use is to compare pieces of DNA from one that is unknown‚ with fragments of DNA
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Purpose: The purpose of this was to determine the concentration of sodium carbonate in an unknown sample by titration. The solution of hydrochloric acid was prepared and standardized using Na2CO3. Observations: Week 1: Standardizing hydrochloric acid using sodium carbonate with bromocresol green indicator Table 1: Titration of sodium carbonate using hydrochloric acid Trial | Mass of Na2CO3 (g) | Burette Reading (mL) | Final Volume of HCl (mL) | | | Initial | Final | | 1
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these pathogens and their related epidemiology has become increasingly more important. The purpose of this study was to identify an unknown bacterium in a controlled laboratory environment over a 5 week period. Utilizing a variety of differential testing and staining methods learned in the microbiology course‚ students were to determine the identity of an assigned unknown organism. Observations were made and recorded each week to narrow down the scope of identification. Data has been presented in
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and Report: Introduction: The experiment was conducted to find the unknown molarity of HCL. The unknown molarity of HCL was found by the use of the titration method and then comparing the results between the titrant and analyte HCL solutions. Molarity is a concentration unit‚ it is defined by the number of moles the total volume of the solution. This experiment included a process called titration‚ used to work out the unknown concentration of a known substance. The process of titration is adding
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identify a metal‚ each with varying amounts of accuracy. In order to test some of these methods‚ thermal expansion‚ density‚ and specific heat were used to identify two small metal rods. The overall purpose of this experiment was to identify an unknown metal using the three previously stated properties. This means that the percent error should be relatively low‚ meaning that there would be little difference between the values measured in our experiment and the true values of the metal. Density
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Application of lactic acid bacteria There are four major categories for the current uses and applications of LAB: food‚ cosmetic‚ pharmaceutical‚ and chemical applications. In food industry Recent years the interest increased in using LAB by using bacteriocin-like inhibitory substances (BLIS) because of their potential use as a natural antimicrobial agent to enhance the food safety of food products and increase the nutritional values of the food. Foods that fermented by LAB such as cheese‚ kimchi
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