Glowing Bacteria Lab Introduction- 1. Transformation involves the transfer of genetic information into a cell by directly taking up foreign DNA from its surroundings. This DNA is then incorporated into the host cell’s own DNA. This transformation usually occurs within plasmids‚ small circular DNA molecules separate from its chromosome. After the recipient cell is infected with the DNA‚ the cell will move on with replication‚ producing offspring with traits encoded by the plasmid. These plasmids
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The purpose of this lab is to isolate a bacterial population from the normal throat flora. A streak plate method will be used to obtain a pure culture of a Gram positive coccus genus of bacteria. Several biochemical tests will be performed to aid in the identification of this unknown bacterium. Biochemical tests are a series of tests used to identify certain bacterium The various tests that are used in this lab are the catalase test‚ oxidase test‚ blood hemolytic test‚ MSA‚ blood agar‚ and PEA/ab
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Bacterial Smears Are Fixed before Staining to? Answer It is important to heat fix the bacterial smear before staining so as to‚ kill the bacteria‚ firmly adhere the smear on to the microscopic slide to prevent washing off during staining‚ and to allow the sample to readily take up the stain. Reference: www2.hendrix.edu What is the purpose of heat- fixing the smear? It helps the cells adhere to the slide so that they can be stained. The purpose of heat fixing is to kill the organisms without
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“Fighting Invisible Killers” Scholastic Scope: The Language Arts Magazine January 2014 Edition‚ pages 5-9 Bacteria surround us every day. These little “bugs” are invisible to the eye and most do not harm us. Many are necessary for us to survive‚ like the bacteria in our stomachs and intestines that help us to digest food. But some bacteria are very dangerous to us. Addie Rereich became very sick in May 2011‚ when she was 11 years old. What started as something that her doctors and mother
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Bacteria are the most numerous type of microorganism found in the rhizosphere of the soil. They produce secondary metabolites which are capable of producing antibiotic which eventually inhibit or kill bacteria. The rhizosphere region of the soil is a highly favorable habitat for the proliferation‚ activity and metabolism of numerous microorganisms. The magnitude of this area depends on the plant and the size of the roots that the plant possesses. Bacteria are among the microorganisms living in the
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Identifying an Unknown Aldehyde or Ketone Introduction The purpose of this lab is to identify an unknown aldehyde or ketone substance using chemical tests. The chemical tests used in this experiment are solubility‚ Schiff‚ Bisulfite‚ and Iodoform tests. Also‚ a 2‚4-dinitrophenylhydrazone derivative synthesis reaction will be completed from which a melting point will be obtained. The chemical test results and the melting point analysis will be compared to the table of compounds given to find the
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Bacterial Contamination April 15‚ 2013 Bacteria Contamination The definition of bacterial contamination is food contamination refers to foods that are spoiled or tainted because they either contain microorganisms‚ such as bacteria or parasites‚ or toxic substances that make them unfit for consumption This is very serious and people should take more precaution‚ food contamination is a serious because it results in foodborne diseases that each year affect an estimated seventy-six million
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Jolyne Piet CHM-221L-02 Lab #2: Experimental Design Isolation of Sucrose: 3.01 g Panacetin were weighed in a 125-mL Erlenmeyer flask‚ and 51mL dichloromethane were added to partially dissolve the Panacetin. The insoluble portion was gravity filtered and air dried to yield 0.45 g of sucrose (15.0 % of original Panacetin). Isolation of Aspirin: The organic filtrate was extracted through a separatory funnel with 32 mL 5% sodium bicarbonate to produce an aqueous layer and a dichloromethane layer
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Gabi Mejia Chem 101 Section ADF Lab 4: Weak Acid Unknown Procedure: When testing the acid‚ use only between 0.2 g and 0.3 g for each trial (get as precise a measurement as you can). The general procedure is to weigh out your acid‚ dissolve it in water‚ add a couple drops of the indicator (phenolphthalein)‚ and then add the sodium hydroxide until you note a color change (from clear to pink). When the color change occurs‚ you have added enough base to completely react with the acid (the endpoint).
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Microbiology 197 Prepared Bacteria Gram Stains (F12) Materials required: * Microscope; clean and properly set up * Immersion oil * Lens paper * Lens cleaning fluid * Microscope drawing forms * Specimens: 1. Bacillus subtilis 2. Staphylococcus aureus. 3. Escherichia coli Procedure: 1. Observe each of slides listed in “Specimens” above. 2. Make your observations using oil immersion (1000X). 3. Using a drawing form draw the organisms
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