Identifying Unknown Bacteria Using Biochemical and Molecular Methods Beginning of Instructor Pages Instructor Pages - - 3 Purpose The purpose of this lab is to introduce a variety of lab techniques to students working on the common problem of identifying an unknown bacterium. This lab helps students develop an understanding of the biochemical and molecular differences in bacteria and introduces the concept of identifying species based on characeristic gene sequences. Students work through
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Identification of Two Unknown Species of Bacteria in Tube #72 Introduction: There are many reasons for knowing the identity of microorganisms. The reasons range from knowing the causative agent of a disease in a patient‚ so as to know how it can be treated‚ to knowing the correct microorganism to be used for making certain foods or antibiotics. This study was done by applying all of the methods that have been learned so far in the microbiology laboratory class for the identification of an unknown bacterium
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Determining the Unknown Microorganism from a Mixed Culture Mylah Ferland‚ Rivier University MICRO 206‚ March 16th‚ 2016 Introduction: An unknown bacterium experiment‚ this experiment was done to discover what bacterium was in a mixed culture sample. This experiment worked with enteric bacteria which are members of the Enterobacteriaceae and live in the intestinal tract (Willey et al.‚ 2014). Also found in Willey et al (2014) is some characteristics of enterics such as they all degrade sugars by
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Antony R 9:00Am-11Am Unknown Number 94 Unknown Results Streptococcus salivarius The first step in my unknown identification was to carry out a gram stain to observe the cell shape and arrangement. My unknown turned out to be gram positive bacteria cocci shaped with long chain cell arrangement. The unknown was gram positive because the cells stained blue-violet ‚this indicate that my unknown has ability to retain crystal violet-iodine after alcohol application on the cells which is a gram
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species of bacteria look similar under the microscope and also have the same staining results (ex. Gram stain). To be able to differentiate between the different species‚ one can look at the metabolic differences (fermentation)‚ as well as the environmental condition differences (temperature‚ pH‚ oxygen requirements). Being able to manipulate these conditions in a controlled environment can help to correctly identify the exact bacteria. Different media can be used to culture and identify bacteria. Some
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The purpose of the unknown bacteria lab assignment was to select an unknown bacteria culture and‚ through a series of metabolic tests‚ identify which bacteria genus resided in the pure culture received. A nutrient broth inoculated with bacterial culture (numbered 45‚ henceforth referenced as U45) was selected and a streak plate was made to isolate a pure culture for use throughout the assignment. From the streak plate‚ several slides were made to determine the morphology of unknown 45. A Gram stain
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Identification and Diagnosis of Unknown Enteric Bacteria in an Infected Patient Using an Enteropluritube Brooke Addario 2/16/2015 BIO 488C - Section J TA – Jacob Karsten Introduction: Enteric bacteria are members of the Enterobacteriaceae family of microorganisms. These gram negative‚ rod shaped‚ facultative anaerobes are found in the intestinal tracts of humans and animals. Enteric bacteria can be pathogenic; however‚ they are also a normal part of human bacterial flora and are therefore
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Unknown Bacterium #22 Purpose The purpose of this experiment was to find the identity two unknown bacteria in the given test tube. Materials •Bacterial Loop •Bunsen Burner • Petri Dish with agar •Crystal Violet •Gram’s Iodine •95% Ethanol •Safrin •Glass Slides •Microscope •Unknown bacteria in test tube Procedure On the first day‚ one plate was streaked qualitatively and left it in the
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isolate and identify two unknown bacteria from a mixed culture provided to us by our instructor. This study was done by applying all of the methods that have been instructed on thus far in microbiology laboratory class. Each test performed‚ provided us with some key information about the unknown microbes in question and how the bacteria function. Materials and Methods Over a two week period‚ eight prepared types of test media were provided to identify the assigned unknown mixed cultures. Not
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Report Unknown Bacteria Staining Gram Acid Fast Spore The first practical for microbiology was staining of an unknown bacteria. Professor Goode provided an unlabeled bacteria in which each individual were to conduct gram‚ acid fast‚ and spore staining tests from memory. When the gram staining test was conducted a positive results was obtained. When views on oil bacteria appeared purple and rod-like. I noted this result and looked at the lab key to see what the results narrowed my bacteria choices
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