Identification of Proteus vulgaris from an Unknown Sample Lakhram Bhisham March 31‚ 2016 01:447:390 General Microbiology TA: Jennifer Goff ABSTRACT This report delineates how unknown #405 was identified as Proteus vulgaris out of a possible seven species of Enterobacteriaceae by applying various tests that are able to distinguish between members of the family. Inevitably‚ the results for many tests are identical across multiple species‚ which is expected due to the organisms’ evolutionary relatedness
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examine the effects of UV light on the growth of different bacteria. Cells are exposed to this radiation for different time intervals to also examine the sensitivity of each cell to each light. The exercise answers questions as to why he lid on the petri dish was removed during exposure time an analyse whether the cells test were equally sensitive to UV light and we also explore the relationship UV has with the time of exposure. The bacteria exposed to UV light for longer would either multiply very
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Date: Wednesday‚ November 16‚ 2011 Holly Lawson - white Title: API 20E Introduction: The general principle of the experiment is to use a battery of biochemical tests for identification of enteric bacteria. This testing system consists of a strip containing 20 chambers‚ each consisting of a microtube and a cupule to allow testing of 20 different tests nearly simultaneously. Materials: inoculating loop‚ test tube containing 5 ml of sterile saline‚ wash bottle of water‚ plastic API
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of bacteria‚ it would mean that therewas some contamination during the pipetting process. It can consequently be said
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what an unknown bacteria was using several different microbiology lab techniques including an API test‚ an oxidase test‚ a gram stain‚ a hanging drop slide‚ and morphology identification. The unknown bacterium‚ which was contaminated with Serratia marcescens‚ was isolated by streaking the bacteria solution to single colonies. The isolated unknown white bacteria‚ had the appearance of circular form‚ convex elevation‚ entire margin‚ elongated cocci. The tests than showed that the bacteria was gram-negative
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flat and raised respectively. Besides that‚ Pseudomonas aeruginosa form irregular colony‚ reaised elevation‚ lobate margin‚ and wet texture. Other bacteria‚ that are Serratia marcescens‚ Proteus vulgaris‚ and Klebsiella aerogenes form circular colony. For S. marcescens and K. aerogenes they have entire margin while P. vulgaris form even margin. S. marcescens form umbonate elevatiob and mucoid texture. While P. vulgaris form raised elevation and smooth texture. Klebsiella so form raised elevation and
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of UV light. The test was a quantitative test to effectively the rate at which changes happen at the distinctive length of UV introduction. As the exposure time of UV light increased‚ the red-pigmented bacteria colonies turned white due to mutation.Therefore‚ both growth and mutation in the bacteria are negatively affected by increasing exposure of UV light. Consequently‚ both development and
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Pseudomonas‚ Cytophaga‚ Ruminococcus albus and Methanobrevibacter ruminantium has long been well recognised due to the importance of cellulose decomposition (Gupta et al.‚ 2011; Wirth & Ulrich‚ 2002). However‚ among the aerobic bacteria‚ the best studied of cellulolytic bacteria species are from the genera Cellulomonas. Lakhundi et al.‚ 2015‚ have been reported that Cellulomonas are Gram positive‚ non-spore forming‚ facultative anaerobic actinomycete that belongs to the Coryneform group of
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much like many other gram-negative bacteria‚ in order to coordinate population behavior during events like invasion‚ colonization‚ and attack. Since they form biofilms on respiratory devices that
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nutrient broth supports the growth of a wide variety of microorganisms while the agar provides a solid substrate on which bacteria can be mechanically diluted and therefore isolated as independent colonies representing different bacterial species. The isolation of independent bacterial species from various environmental sources is important in all branches of microbiology since bacteria are ubiquitous and live in microbial communities of mixed populations. Populations in microbial communities or ecosystems
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