diagnostic bacteriology Dr.T.V.Rao MD Dr.T.V.Rao MD 1 Identification of Microorganisms • For many students and professionals the most pressing topic in microbiology is how to identify unknown specimens. • Why is this important? • Labs can grow‚ isolate and identify most routinely encountered bacteria within 48 hrs of sampling. • The methods microbiologist use fall into three categories: ♣Phenotypic- morphology (micro and macroscopic) ♣Immunological- serological analysis ♣Genotypic- genetic
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Tiffany Ward Scheme For Unknown 2A/B Possible Ions: Cations: Na+‚ K+‚ NH4+ Anions: OH-‚ Cl-‚ NO3-‚ SO42-‚ HSO4- 1.Describe Sample Phase‚ color‚ odor‚ crystal shape‚ amorphous‚ gel-like‚ needle-like‚ powdery‚ etc. 2.Tests on original sample (i) Flame test bright orange Na+ purple K+ no color NH4+ (ii) NH4+ test Place a small portion of original unknown sample in a test tube or spot plate‚ then add a few drops of 1M NaOH solution. At the same time suspend a piece of red
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Unknown Microorganisms May 1‚ 2013 This paper is based on an experiment done in the laboratory over a period of time using the standard method of identifying an unknown microorganism relying on features such as morphology‚ arrangements and biochemical reactions. It was done in succession with each step complimenting the other. At the end of the experiment three microorganisms Bacillus azotoformans‚ Staphylococcus aureus‚ and Escherichia coli was identified and one result was inconclusive. The
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Unknown Lab Report Microbiology Unknown A Sonia Kabra November 26‚ 2014 Introduction There are numerous reasons for identifying unknown bacteria. Some of these organisms have distinct qualities that set them apart from one another‚ such as the exposure to certain environments. Through out the semester in the laboratory‚ we are able to encounter some of the few microorganisms that we as humans have come into contact with. With the knowledge gained from the sessions in the laboratory‚ we can now
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Analysis of an Unknown Mixture Performed: 2/13/14 Submitted: 3/3/14 Abstract: The purpose of this experiment was to separate and identify the two unknown compounds. The sample used was 105 and it contain 2-propanol and 2-heptanone. IR spectrums were taken of the contents of the second and sixth test tubes and showed the presence of an OH functional group and a small amount of ketone in test tube two and a large presence of ketone in test tube six. The average boiling points of the test tubes‚ 73°C
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Unknown Lab Report April 25th‚ 2006 Introduction The purpose of this lab was to identify two unknown bacteria cultures using various differential tests. The identification of these unknown cultures was accomplished by separating and differentiating possible bacteria based on specific biochemical characteristics. Whether the tests performed identified specific enzymatic reactions or metabolic pathways‚ each was used in a way to help recognize those specifics and identify the unknown cultures
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My journey to the unknown started about three weeks before the end of my microbiology class at Brunswick Community College. This journey started out with a media plate cultured with an unknown species of bacteria. I was then instructed by my professor to use all of my previous knowledge to identify the unknown bacterial pathogen. This lab led me to master my skills of using the Gram stain‚ testing for catalase‚ testing for coagulase and using the oxacillin screen. Now I am going to take you with
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Tanti Lim Thurs AM Unknown Project I. Introduction The purpose to this lab was to isolate and identify two unknown bacteria from a mixed culture provided to us. This study was done by applying all of the methods that have been instructed on thus far in microbiology laboratory class. Each test performed‚ provided us with some key information about the unknown microbes in question . The identification of unknown bacteria is a time honored part of microbiology courses. It will challenge
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discuss the results of an experiment being conducted on the effect that antibiotics have on the growth of bacteria. This will be done through the use of microbiology. Microbiology is a science in the department of biology‚ particularly microorganisms. These include; bacterium‚ fungi‚ protozoa‚ algae and viruses. This is an important subject to study as microorganisms can be found everywhere‚ covering all matters of different surfaces even including the human body. Bacterium‚ or bacteria‚ is an important
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Purpose: To identify an unknown bacterial specimen using basic laboratory technique and biochemical tests. The unknown bacteria will be one of the following: Enterococcus faecalis‚ Staphylococcus saprophyticus‚ Escherichia coli‚ Enterobacter aerogenes‚ Proteus vulgaris‚ Salmonella [I assume typhimurium]‚ or Shigella [either flexneri or sonnei‚ we used both in our lab during the semester]. Procedure {and observations}: Observe bacterial colony morphology. {Colonies are large‚ beige or
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