following agar mediums: Blood Agar (pg 168)‚ EMB Agar (pg 170)‚ Mannitol Salt Agar (MSA)(pg 172) )‚ MacConkey Agar (pg 174)‚ and PEA Agar (pg 176) to answer the following: 1. What does the blood agar select for? Blood agar allows distinction among bacteria based on their ability to lyse red blood cells (hemolytic activity). 2. What color is the blood agar? Blood red color. 3. What are the 3 types of blood agar results and how can you recognize them? Beta hemolysis‚ which is the complete
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Gram-positive cocci in clusters (catalase positive). The coagulase test is used to differentiate Staphylococcus aureus from coagulase-negative staphylococci. The test uses rabbit plasma that has been inoculated with a staphylococcal colony. The tube is then incubated at 37 degrees Celsius for 1½ hours. If negative then continue incubation up to 18 hours. * If positive (i.e.‚ the suspect colony is S. aureus)‚ the serum will coagulate‚ resulting in a clot (sometimes the clot is so pronounced that the
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Horack’s article “Staphylococcus epidermidis”‚ S. epidermidis is described as “gram-positive cocci bacteria that are part of the normal flora on the skin and nasal passages.” The article goes on to say that the species was originally named Staphylococcus Albus by microbiologist Rosenback in 1884. When viewed under a microscope S. epidermidis will appear in chains‚ pairs‚ or grape-like clusters (Horak 1). Taxonomically‚ the species S. epidermidis falls in the genus Staphylococcus‚ which is in the
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CHAPTER TWO Beneficial bacteria in the environment and their uses. In today’s world‚ the environment and its related issues are steadily gaining a lot of importance. Some bacteria are helpful and are used to obtain balance in the environment. It has been seen that helpful bacteria are useful in dissolving organic sludge from water‚ breaking down the growth of algae‚ reducing the various noxious odours such as hydrogen sulfide odours‚ reducing ammonia levels‚ promoting faster growth of fish in the
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Answer the following: Mimi Meowz is a large designer-clothing company that sells mainly to large retail department stores‚ as well as to smaller boutique shops. The company requires its salespeople to use notebook computers to enter orders from customers. This information is then transmitted to its warehouses‚ where orders are shipped on a first-come‚ first-served basis. Unfortunately‚ the stores are aware of this shipping policy and are extremely competitive about which one of them will offer a
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Double unknown identification of a mixed culture of Gram-negative and Gram-positive bacteria 2. Author: Nick Fiore‚ University of Kansas‚ Biology 402‚ Fall 2014 3:00pm room 6040 3. Abstract: The purpose of this experiment was to isolate two unknown bacteria and perform a series of selective and differential tests to correctly identify each. After the bacteria was isolated a series of differential and selective tests following the dichotomous key attached were used to identify each bacteria. The Gram-positive
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140 Matthew Tuthill Unknown Lab Report Introduction There are many reasons for knowing the identity of microorganisms. The reasons range from knowing the causative agent of a disease in a patient‚ so as to know how it can be treated‚ to knowing the correct microorganism to be used for making certain foods or antibiotics. This study was done by applying all of the methods that I have been learned so far in the microbiology laboratory class for the identification of an unknown bacterium. The purpose
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GAUTENG DEPARTMENT OF EDUCATION SENIOR SECONDARY INTERVENTION PROGRAMME English FAL Grade 12 SESSION 15 (LEARNER NOTES) LITERATURE: STUDYING DRAMA Learner Note: It is difficult to discuss a specific DRAMA in this session‚ since all schools are not doing the same prescribed work. This session will focus more on introductory notes on the study of drama and will include extracts from some of the prescribed plays. It is‚ however‚ very important that you know the basics of studying drama – once you
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referred to as the unknown. Materials and Methods A test tube with an unknown microorganism will be retrieved. Once the test tube is retrieved‚ a steak for isolation will be completed in order to produce isolated colonies of the organism on an agar plate. The unknown test tube with the bacteria is flame sterilized using a Bunsen burner. Once the bacterium test tube has been flame sterilized‚ a flame sterilized inoculating loop will be used to gather
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a. The bacteria that causes botulism are found in soil‚ water‚ animal guts and dung. When the spores exposed to low oxygen and certain temperature‚ they produce botulinum toxin‚ able to germinate‚ metabolize‚ food sources through an aerobic respiration to produce toxin. The toxin blocks the release of acetylcholine from motor nerve endings causing paralysis symptoms. The botulism either food born‚ eating contaminated home-canned foods where the fermentation takes place. or through abrasion in the
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