I. Title Identification of an Unknown Plasmid In this experiment‚ we determined the phenotypic capability of an unknown plasmid along with its size. With the use of gel electrophoresis‚ we analyzed the gel photograph by using a standard DNA marker‚ Lambda HindIII‚ and came to a conclusion based on our results. II. Abstract Two experiments were done to identify an unknown plasmid. The success of these experiments came from the use of modern day technology involving gel electrophoresis
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Microbiology 197 Prepared Bacteria Gram Stains (F12) Materials required: * Microscope; clean and properly set up * Immersion oil * Lens paper * Lens cleaning fluid * Microscope drawing forms * Specimens: 1. Bacillus subtilis 2. Staphylococcus aureus. 3. Escherichia coli Procedure: 1. Observe each of slides listed in “Specimens” above. 2. Make your observations using oil immersion (1000X). 3. Using a drawing form draw the organisms
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Unknown Report Emily Juhl Section – 3 April 15‚ 2013 Unknown #8 Family: Enterobacteriaceae Genus: Serratia Species: Marcescens Abstract Microorganisms are constantly finding ways to resists to antibiotics. For this reason‚ it is important to test and observe unknown organisms in the lab to continually improve the health and well being of society. The objective of this report was to first isolate a single colony of an unknown culture on a LB agar plate. From
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Viruses can infect all types of cells including plant‚ animal‚ protozoa‚ fungi‚ and bacteria. Virus composition is unique and does not resemble a living cell because they only contain the necessary parts to enter and leave an infected cell. A virus is a minute parasite (10 to 100 times smaller than bacteria) that is unable to reproduce by itself; however‚ once it infects a vulnerable cell a virus can make the cell’s inner workings produce viruses on its behalf. Viruses typically have either RNA
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Effects of Different Antibiotics on Bacteria Because of their small size‚ rapid rate of reproduction‚ and relatively simple structure‚ bacteria are excellent subjects for laboratory studies. This investigation will introduce you to techniques of microbiology that will be of use to you throughout your biological work. Procedure 1. Heat the test tubes of sterile agar medium in the water bath until the agar melts. 2. Remove the test tubes from the water bath. Let them cool enough to hold in your
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Bacteria: a benefit or a hazard? Bacteria is something we are all reminded of on a daily basis by merely switching on our televisions where we are bombarded with advertisements for both ‘good’ and ‘bad’ bacteria. Contrary to the view of the past when only so-called ‘bad’ bacteria was ever talked about‚ so what has changed? This essay will address the facts about bacteria including methods used in identifying bacteria as well as looking at specific examples of how they can be both helpful and harmful
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over 99% of bacteria or antibiotics aren’t discovered. However‚ recently a new antibiotic called teixobactin was discovered. This antibiotic prevents the synthesis of a cell wall by binding to a motif of lipid II and lipid III molecules. The goal of this experiment was to discover how teixobactin interact with bacteria and how it is able to effectively kill pathogens. A device called the iChip was used to discover teixobactin. The iChip helped to isolate and grow uncultured bacteria. Multiple iChips
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Sarah Burns Evolution of Plague Bacteria The Bubonic Plague otherwise known as the Black Death‚ has gotten most of its attention from medieval paintings‚ poetry‚ and journals of revulsion. The real horror‚ was the disturbing biological evidence of the bacteria that caused all the pandemics‚ known as Yersinia pestis. The pathogen got its name from the two investigators Yersin and Kitasato. In 1894‚ Yersin was known as the main investigator (ergo. Named after him)‚ he claimed that the mice/rats were
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Flesh-eating bacteria strikes in Florida On June 16th‚ 26 year-old Cason Yeager went for a swim in Florida in Hernando County and later died from contracting the flesh-eating bacteria‚ Vibrio Vulnificus. This bacteria can be found worldwide‚ most particularly in the warm coastal regions but there have been reports in other parts of the world in non-warm waters. According to the CDC in 2013‚ the case count of the vibrio vulnificus was 104 in thirteen states with six hospitalizations. No deaths‚ but
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Kenny Vo Microbiology Dr. Arora 10 December 2012 Flesh Eating Bacteria Necrotizing Fasciitis is a rare bacterial disease that causes the rapid decay of the fascia. The human body is said to be compose of approximately a hundred trillion cells‚ connective tissue contributes to about forty five percent of the total cell weight. It was nicknamed the flesh eating bacteria because the victims injuries would have a rotting appearance. What are the pathogens that cause this disease? Necrotizing
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