Microbiology Unknown 5/7/2013 Microbiology Unknown #94 The purpose of this report was to identify an unknown bacterium by applying methods that were previously learned and conducted in microbiology lab class. It is possible to identify a mixed culture by running experiments on the unknown bacteria and taking what we already know and applying it to the results. It helps to slowly eliminate any bacteria that do not correspond with the results of certain tests. An unknown test tube labeled 94 was given
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9/20/2013 Methicillin-Resistant Staphylococcus aureus Methicillin-Resistant Staphylococcus aureus also known as MRSA is a bacteria frequently found on the skin of generally healthy people. MRSA is usually spread through skin to skin contact or contact with an object containing the bacteria. Most of the time you may be carrying the bacteria but you are not ill. Mostly people with weak immune systems and have been in health care centers
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Bacterial Smears Are Fixed before Staining to? Answer It is important to heat fix the bacterial smear before staining so as to‚ kill the bacteria‚ firmly adhere the smear on to the microscopic slide to prevent washing off during staining‚ and to allow the sample to readily take up the stain. Reference: www2.hendrix.edu What is the purpose of heat- fixing the smear? It helps the cells adhere to the slide so that they can be stained. The purpose of heat fixing is to kill the organisms without
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Staph infections are caused by a staphylococcus bacterium‚ Staphylococcus aureus. S. aureus is a purple‚ cocci shaped cell with its morphological arrangements in grape-like clusters‚ it is determined by a bacteriological stain‚ known as the Gram stain. This determines it to be a gram positive bacterium. When grown on bacteriological media‚ S. aureus appears as a large white to golden colony. It is not very fastidious but it grows well‚ either aerobically or under anaerobic conditions and produces
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Analysis of Unknown Colony Morphology Color- light yellow pigmentation Form- circular Elevation- convex Margin- entire [staphylococci] DNase Test This test is used to detect if the bacteria contains any deoxyribonuclease activity. Because no color change was observed from blue to clear my unknown bacteria displayed a negative result. Blood agar plate This test is used to detect the hemolytic activity in the bacteria. A darkish green color on the media around the bacteria would represent
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Molecular Characterization of Methicillin-Resistant Staphylococcus aureus (MRSA) Nasal Colonization and Infection Isolates in a Veterans Affairs Hospital Staphylococcus aureus (S. aureus) belongs to the genus Staphylococcus. It is Gram-positive‚ cocci shaped anaerobic bacteria that forms clusters and produces catalase‚ which catalyzes hydrogen peroxide to water and oxygen. It contains a cell wall structure (including peptidoglycan and teichoic acid) and a low G + C content of DNA between 30-40 mol
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BACTERIA Period: 4 Characteristics: 3 major shapes Cocci Basilli Spirilla 3 major components Mesosomes flagella Plasmids Growing Up: Bacteria can obtain energy through phototrophs(sunlight)‚ lithotrophs(inorganic compounds)‚ and organotrophs(organic compounds) Marriage/Reproduction Binary Fission: The process by which all bacteria reproduce. It results in the separation of a single cell into two. Transformation: genetic alteration
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risk factor for other diseases. In light of these significant numbers‚ our group chose to explore the relationship of dietary fiber to aid in the prevention and treatment of obesity‚ therefore also reducing the incidence other diseases. Our focus was on making a hot meal with a simple modification to increase the dietary fiber available. The original recipe is a white rice pilaf with the adjustment being made with a substitution of brown rice. This change will boost the fiber intake from 0.8g per
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2. Material and methods 2.1 Sample collection The following 14 samples of Staphylococcus aureus were used in this study. All samples were obtained from food contact surfaces at three food premises in University of Putra Malaysia (UPM). Samples were collected from the surface of each material according to standard swabbing techniques. The collection was carried out during weekdays (15:00-17:00) after finished the preparation of foods. Sterile swabs were removed from the coded test tubes that contain
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the unknown organisms in the mixed culture was limited to bacteria that we had worked with previously in lab. Initially a Gram stain was conducted in order to distinguish the unknown bacterium as a Gram-positive and/or a Gram-negative organism (Lancaster and Bennett‚ 2012; Kellenberger‚ 2001). Based upon the results‚ both Gram-negative and Gram-positive bacteria were observed in the unknown mixed culture (Table 1 and Table 2; Kellenberger‚ 2001). In order to isolate the two different bacteria‚ colonies
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