As a result of the tests ran‚ I was able to identify the unknown bacteria. For Unknown A‚ I ran six tests. I first isolated the bacterium from the second bacterium and found a clear growth (Table 1‚ Figure 1). Secondly‚ I ran a gram stain and found a gram positive‚ cocci bacterium (Table 1‚ Figure 2). Third‚ I ran a catalase test in which was negative (Table 1). From here‚ I determined a starch hydrolysis test would be necessary to distinguish between different bacteria. The result was negative (Table
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Identification of A Mixed Culture Unknown An experiment such as this one serves the purpose of allowing us‚ the students‚ to apply what we already know about any organism and any laboratory procedure to the difficult task at hand. It is possible to identify a mixed culture by running familiar experiments on the unknown bacteria and taking information already known about specific bacteria and applying it to the results. This helps to slowly eliminate any bacteria that do not correspond with the
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Pure Culture Techniques In this first lab‚ you will be learning some very fundamental and important techniques. As is the case with most things‚ shorts cuts usually get you in trouble. This is especially true in Microbiology. The techniques you will be learning tonight‚ if mastered correctly‚ will make your life and learning experience in Microbiology much easier‚ if you don’t pay attention and practice these techniques incorrectly‚ well then……? Today you will be learning the following techniques:
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Coccus is a term used to describe a type of morphology that helps us distinguish different kinds of bacteria. The cell’s morphology is only one of the many ways we can differentiate the different species. Looking at these bacterium underneath the microscope is an important step to determine which kind of species it is. All cocci bacteria are not gram positive‚ but this lab will examine only this kind because of their influence in human health. Staphylococcus Aureus is a gram-positive coccus bacterium
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Thus‚ identification of microbes is a process of elimination based on logic and carefully-performed tests to determine the capabilities of the unknown microbe. The pieces of evidence used in the microbiology lab are your observations of the shape‚ morphology‚ Gram reaction‚ and the results from biochemical tests performed using a pure culture of an unknown organism. Results are compared to tables of known results for all bacteria with similar shape‚ morphology and Gram reaction to see where similarities
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within the mucous coating on the intestine. They don’t damage their range-land. Dr. Gilmore along with B. Faecalis‚ the research exhibited "that it devotes many of its genetic material to digestive enzymes in which take place on the surface of the cell.” That suggests it can be breaking substances for other additional organism‚ most likely aiding certain sorts of microbial neighbors. Enterococcus might be getting the chance to move nearer to the small intestine or even the urinary tract that is to
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REVIEW OF RELATED LITERATURE This investigatory project aims to make use of rambutan peeling extracts that is high in phenolic compounds such as tannin and saponin that exhibits antioxidant activity and antibacterial activity against pathogenic bacteria. Although the seeds contain such compounds the peeling exhibit greater amount of the said compounds. Rambutan is a very popular fruit cultivated in the Philippines and it is eaten in large amounts. The rambutans rind is quickly discarded and
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Introduction The main purpose of root canal treatment is the elimination of microorganism from root canal system (1‚2). Microorganisms and their byproducts are regarded as the primary etiological factors of periapical diseases and pulpal necrosis (3‚4‚5). Microbial reduction obtained by mechanical instrumentation‚ irrigation‚ using intracanal medicaments and adequate filling of root canal (6). Failure of the root canal treatment is the result of persisting microorgansims in the root canal system
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quantification of indigenous intestinal microbiota. The success of such analysis requires effective methods for the extraction of faecal bacterial DNA. Three extraction methods were assessed for their effectiveness in extracting Escherichia coli and Enterococcus spp. bacterial DNA from human faecal samples: an in-house phenol/chloroform extraction‚ and two commercially available kits‚ ExtractMaster (Epicentre Biotechnologies) and UltraClean (Mo Bio Laboratories) faecal DNA extraction kits. Real-time PCR
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Lab Report: Antibiotic Evaluation by the Kirby-Bauer Method Introduction Chemical antimicrobial agents are chemical compounds capable of either inhibiting the growth of microorganisms or killing them outright. Those which are taken internally to alleviate the symptoms of or promote healing from disease are called chemotherapeutic drugs‚ and among these is a class of compounds called antibiotics. In order for a chemotherapeutic drug to be classed as an antibiotic‚ it must be produced by
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