IDENTIFYING GRAM POSITIVE COCCI As mentioned in Exercise 8‚ “Identifying Gram Negative Rods”‚ identifying bacteria is a common activity in the microbiology lab. Like the game Clue™‚ each time you gather a piece of information to solve the mystery‚ you gather some information that supports some identities and eliminates others from contention. In the lab‚ the process continues as you gather more information until only one microbe remains and all others have been eliminated as possibilities. Thus
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Identifying the Unknown Bacteria: Bacillus cereus Brittany Moy Coastline Community College Abstract Bacillus cereus is a Gram-positive‚ facultative aerobic‚ spore-forming‚ rod-shaped bacterium that is widely distributed among the environment. Introduction The reason for identification of unknown bacteria was to help students recognize different bacteria through different biochemical tests and characteristics. This is important in the medical field because identification of unknown bacteria
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The unknown bacteria A and bacteria B have to be identified by its genus and species. First both bacteria had to be inoculated into a TSA agar media using the streak plate method. Four quadrants were drawn‚ so that the bacteria could be isolated as much as possible. Each bacteria was inoculated into two different plates‚ so that one could be incubated at 37 degrees Celsius and the other at 25 degrees Celsius. Bacteria B‚ which was incubated at room temperature showed red colonies throughout its media
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a known quantity of solid dissolved in solution. It may also be used to calculate the molar mass of an unknown acid or base. Purpose: I. Prepare a standard solution of sodium hydroxide II. Standardize a sodium hydroxide solution by using potassium hydrogen phthalate (KHP) III. When given whether the acid is monoprotic‚ diprotic‚ or triprotic ‚ determine the molar mass of the unknown organic acid. IV. Calculate the Molarity of the Soft drink. Materials: * 50 mL Buret
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difference between a gram positive bacteria and gram negative bacteria is the differences in cell wall composition. Prokaryotes known as eubacteria have three basic forms: rods‚ cocci and spiral. The bacterial cell wall is the single most important contributor to cell shape. In addition to shape of cell wall‚ presence or absence of flagellum‚ and if present‚ positions of flagellum‚ the eubacteria can be classified according to Gram Stain. First and foremost‚ gram positive bacteria are by
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Exercise 5 Introduction: Many different species of bacteria look similar under the microscope and also have the same staining results (ex. Gram stain). To be able to differentiate between the different species‚ one can look at the metabolic differences (fermentation)‚ as well as the environmental condition differences (temperature‚ pH‚ oxygen requirements). Being able to manipulate these conditions in a controlled environment can help to correctly identify the exact bacteria. Different media
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Introduction: Identification of bacteria is significant because they are not only beneficial but potentially hazardous as well. The identification of bacteria can lead to breakthroughs in healthcare regarding treatment of old and new diseases alike. In addition‚ Identifying bacteria can furthermore be utilized in various other areas to knowing the causative agent of a disease in a patient‚ so as to know how it can be treated‚ to knowing the correct microorganism to be used for producing certain foods
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Unknown Bacterium #22 Purpose The purpose of this experiment was to find the identity two unknown bacteria in the given test tube. Materials •Bacterial Loop •Bunsen Burner • Petri Dish with agar •Crystal Violet •Gram’s Iodine •95% Ethanol •Safrin •Glass Slides •Microscope •Unknown bacteria in test tube Procedure On the first day‚ one plate was streaked qualitatively and left it in the
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purpose of the unknown bacteria lab assignment was to select an unknown bacteria culture and‚ through a series of metabolic tests‚ identify which bacteria genus resided in the pure culture received. A nutrient broth inoculated with bacterial culture (numbered 45‚ henceforth referenced as U45) was selected and a streak plate was made to isolate a pure culture for use throughout the assignment. From the streak plate‚ several slides were made to determine the morphology of unknown 45. A Gram stain‚ used
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isolate and identify two unknown bacteria from a mixed culture provided to us by our instructor. This study was done by applying all of the methods that have been instructed on thus far in microbiology laboratory class. Each test performed‚ provided us with some key information about the unknown microbes in question and how the bacteria function. Materials and Methods Over a two week period‚ eight prepared types of test media were provided to identify the assigned unknown mixed cultures. Not
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