Identification of Unknown # 15 Abstract. One of the most fundamental differential staining techniques used in the study of bacteriology is gram staining. There are two main types of bacteria‚ gram negative and gram-positive. The purpose of this experiment was to perform a variety of tests to identify the bacteria contained in the unknown sample labeled number 15. The following are the tests that were used to identify the two different bacteria. The
Premium Bacteria Microbiology Gram staining
Gram Staining of Bacteria Since bacteria is really small microbiologist use stains to help them see bacteria more clearly under the microscope. Many of the stains they use color the bacteria cells directly and are called direct stains. Bacteria cells have a slightly negative charge while direct stains have a slightly positive charge that helps the stain bin to the bacteria. The strength of the binding from the stains depends on the make-up of the cell wall itself. Gram staining is the most helpful
Premium Staining Bacteria Gram staining
average‚ there are about 125 million rod cells in the human retina. Rod cells are almost entirely responsible for night vision. Rod cells do not process fine details or color differences‚ but are highly sensitive to light. A single flash of light is all that is require to produce a response from a rod cell. Rod cells function as specialized neurons that convert visual stimuli in the form of photons (particles of light) into chemical and electrical stimuli. Rod cells are responsible for perceiving
Premium Retina Eye Photoreceptor cell
UNKNOWN LAB REPORT UNKNOWN NUMBER 54 Ron Williams 7-28-05 Bio 205 Prof. Curlee Fall 2005 Purpose There are many reasons for knowing the identity of microorganisms. The reasons range from the knowing the causative agent of a disease in a patient‚ so as to know how it can be treated‚ to knowing the correct microorganism to be used for making certain foods or antibiotics. This study was done by applying all of the methods that have been learned so far in the microbiology laboratory
Premium Bacteria Enzyme Glucose
Name SOLUBILITY CURVES Answer the following questions based on the solubility curve below. Which salt is least soluble in water .. at 2O° C? 2. How many grams of potassium chloride can be dissolved in 200 g of water at 80° C? IO 3. At 40° C‚ how much potassium _ __nitrate coin be dissoiu$tl ^n 30D.g of water? ------W- ’1 80 70 ...- O --60 0 5© 40 4. Which salt shows the least change 30 In solubility from 0° - 100° C? 20 10 At 30° C‚ 90 g of sodium
Premium Solubility Ammonia Solution
identification of two unknown bacteria. An unknown labeled as number 3 was given out by the lab instructor. The methods that have been lectured and absorbed pertaining to identifying
Premium Bacteria Microbiology Gram staining
Discussion The possible identity of the unknown organisms in the mixed culture was limited to bacteria that we had worked with previously in lab. Initially a Gram stain was conducted in order to distinguish the unknown bacterium as a Gram-positive and/or a Gram-negative organism (Lancaster and Bennett‚ 2012; Kellenberger‚ 2001). Based upon the results‚ both Gram-negative and Gram-positive bacteria were observed in the unknown mixed culture (Table 1 and Table 2; Kellenberger‚ 2001). In order
Premium Bacteria Microbiology Escherichia coli
by aseptically inoculating a tube of phenol red sucrose broth‚ and a tube of phenol red arabinose broth with the unknown culture and incubating at 37 degrees Celsius for 48 hours. After incubation‚ the two tubes were examined for color change. The eighth test was to find out if the bacteria in question had flagella. The motility test was performed by aseptically inoculating the unknown bacteria into a tube of TSA broth and allowing it to incubate for 24 hours at 37 degrees Celsius. After the TSA
Premium Bacteria Microbiology Microorganism
Introduction: The gram stain is one of several laboratory procedures that can be used to narrow down the identities of unknown bacteria. Bacteria have three different shapes; cocci‚ bacilli‚ and spirilla. Since bacteria pretty much have the same reflective index as water‚ a bacteria cell must be dyed so that these shapes can be seen. Materials: Petri dish Dropper Cleansing solution Slides Bibulous paper Inoculation loop Crystal violet dye‚ Iodine Acetone Safranin Water from the sink
Premium Staining Gram staining Microbiology
Medical Mycology: Yeast and Pneumocystis| Reading Assignment:|Mahon‚ Chapter 10‚ pgs 215-219‚ Chapter 27‚ pgs 626-629‚ 634-636‚ Appendix B Lecture Notes: Medical Mycology| |U of W Tutorial on Mycology (organisms listed in objectives)‚ www.medtraining.org[->0]| _____________________________________________________________________ 1. Discuss the difference between yeasts and molds. Fungi seen in the clinical laboratory can be generally separated into two groups based on the appearance of the
Premium Yeast Fungus Candida albicans