cystic fibrosis prone to infections with Pseudomonas aeruginosa? Pseudomonas aeruginosa is an opportunistic infection that usually does not affect healthy patients‚ but is particularly detrimental in patients with cystic fibrosis. This is because they are antibiotic resistant to a certain extent. Paired with this‚ patients with cystic fibrosis have a decreased ability to produce mucous membranes‚ making it easier for biofilms associated with P. aeruginosa (Lyczak et al 2002). This along with their
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spectrometry. We will do this by making 2‚ 4 a DNPH derivative and checking the melting points. Theory: By Using specific methods of compounds detection‚ we can match an unknown compound with a known compound because similar compounds will display similar characteristics. In this experiment‚ identifications of the unknown ketone was accomplished using thin layer chromatography‚ melting point‚ and NMR spectrometry. Thin layer chromatography is very quick but sensitive way of determining the components
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I. Title Identification of an Unknown Plasmid In this experiment‚ we determined the phenotypic capability of an unknown plasmid along with its size. With the use of gel electrophoresis‚ we analyzed the gel photograph by using a standard DNA marker‚ Lambda HindIII‚ and came to a conclusion based on our results. II. Abstract Two experiments were done to identify an unknown plasmid. The success of these experiments came from the use of modern day technology involving gel electrophoresis
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EXPERIMENT 1 IDENTIFICATION OF UNKNOWN COMPOUNDS Date of experiment : 7th August 2012 Venue: ME204 INTRODUCTION Chemical reactions are regularly categorized into 3 types: oxidation-reduction (redox reaction)‚ precipitation (double displacement) and acid-base reaction (double displacement) Type 1: Oxidation-Reduction Reactions Oxidation-reduction processes include the movements of electrons form oxidants to reductants‚ which lead to increases in oxidation
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The Identification of Two Unknown Species of Bacteria in Tube #72 Introduction: There are many reasons for knowing the identity of microorganisms. The reasons range from knowing the causative agent of a disease in a patient‚ so as to know how it can be treated‚ to knowing the correct microorganism to be used for making certain foods or antibiotics. This study was done by applying all of the methods that have been learned so far in the microbiology laboratory class for the identification of an
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Pseudomonas aeruginosa Pseudomonas aeruginosa is the bacteria of my initial unknown project. Determining which bacteria I had was completed by many steps. The one fact that I had‚ was that it was a gram negative bacteria because on the gram stain it appeared pink. Its cell wall is composed of a plasma membrane‚ periplasmic space‚ peptidoglycan and an outer membrane (lipopolysaccharide and protein). By looking at the agar plate it was easy to tell that it was not a swarmer and it did not have any
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Identification of Unknown Organic Compound by Melting Point (M.P)‚ Boiling Point (B.P) and Infrared Spectroscopy Methods and Background The main objective of this lab is to identify the given unknown organic compounds with various methods. Identification of an unknown compound is important to perform through the process of melting point (M.P)‚ boiling point (B.P) and Infrared spectroscopy (I.R). Index of Hydrogen deficiency (IHD) and elemental analysis makes an experiment more efficient in
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become increasingly more important. The purpose of this study was to identify an unknown bacterium in a controlled laboratory environment over a 5 week period. Utilizing a variety of differential testing and staining methods learned in the microbiology course‚ students were to determine the identity of an assigned unknown organism. Observations were made and recorded each week to narrow down the scope of identification. Data has been presented in the tables‚ charts and drawings herein and reflect
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MIC 230 Unknown Identification Report The objective of this experiment was to identify an organism from a mixture of two unknown bacterial species. In order to accomplish this‚ I first plated my unknown mixture on Tryptic Soy Agar (TSA)‚ Columbia Naladixic Acid (CNA)‚ and MacConkey’s Agar (MAC) plates. After 48 hours of incubation‚ it was unclear that two different bacterial colonies had grown on my TSA plate. Only one type of colony was evident. However‚ it was apparent that I had successfully
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Identification of an Unknown Organic Acid Chem 1211K Lab Drawer #15 Wednesday November 13th‚ 2013 Unknown Number: 3334025-CF13 Table of Contents Pg. # Abstract 3 Experimental Report 4-7 Results and Discussion 7-12 Conclusion 13 **pKa Graph 14 Abstract Identifying this organic acid was an extensive task that involved several different experiments. Firstly‚ the melting
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