Experiment H: Identification of an Unknown Organic Compound The objective of this lab was straightforward. We were given an unknown compound and we were to perform an IR spectroscopy and as well as NMR spectroscopy. With the IR spectroscopy‚ I was able to name the functional groups I have on my compound and further confirmed my assumptions by looking at the NMR spectroscopy after. The unknown number I was given was number 203. The molecular weight of the compound was 121. From the molecular weight
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Grainy and shiny white Becoming all liquid 125 Clear B. Melting point of unknown #42 1st trial Temperature (¡ÆC) Observation Unmelted Shiny light yellow crystal First liquid appear point 99 Grainy yellow Becoming all liquid 101 Clear 2nd trial Temperature (¡ÆC) Observation Unmelted Shiny light yellow crystal First liquid appear point 95 Grainy yellow Becoming all liquid 97 Clear C. Melting point of mixture (Unknown + Benzil) Temperature (¡ÆC) Observation Unmelted Shiny light yellow
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different evidences on the isolation of Pseudomonas from the soil experiment. Firstly‚ the plate count agar is a medium for the enumeration of viable organisms in food‚ water‚ waste water and also from clinical samples‚ and thus it is non-selective to any species. Whereas the CFC agar is a selective agar which contains reduced amounts of cetrimide but also cephaloridine and fucidin are added to allow the isolation of most Pseudomonas species. If the Pseudomonas species are present‚ a blue-green or
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TITLE OF PROJECT: Effects of Chemoattractants of Pseudomonas Aeruginosa Through a Capillary Tube. PROPOSED COST: $1764.90 ABSTRACT: This project will show how strong certain bacteria that are commonly found in biofilms attract to Pseudomonas aeruginosa through a capillary tube. It will test ten bacteria to see which bacteria have more chemoattractants with Pseudomonas aeruginosa . These bacteria are all motile‚ so to come together to form a biofilm‚ they must lose their ways of
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of biological system is usually preferred as the pH system‚ which determines the pH level of dilute aqueous solutions. In this laboratory‚ the data collected from the experiment will be used to graph the titration curves which help to identify an unknown amino acid. IntroductionAmino acids are simple monomers which are strung together to form proteins. Amino acids play a key cellular role in structure and function. Proteins themselves participate in nearly every physiological event in the cell. Since
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Pseudomonas taiwanensis – Enrichment‚ Isolation and Identification Microbiology 521 2/10/12 Purpose: The purpose of this experiment was to enrich Pseudomonas bacteria‚ isolating a species of Pseudomonas and identifying it using phenotypic properties and DNA sequencing as an existent or completely new and undiscovered species of Pseudomonas. Overview: Genus Pseudomonas is a chemoheterotrophic bacteria found in soil and water. They are Gram negative‚ motile‚ paired rods that are also oxidase-positive
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Unknown Microorganism: #5 Report Callie Schalk Microbiology Professor Quave November 24‚ 2015 The unknown microorganism #5 is Pseudomonas aeruginosa. It can survive in a variety of environments and at temperatures found in clinical settings (Murray et al‚ 1999). Clinical setting is referring to temperatures such as those in the hospital environment. P. aeruginosa can also survive in aqueous environments. Pseudomonas aeruginosa is found at several different sources. Nosocomial
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and applications of biosurfactant produced by Pseudomonas cepacia using de-oiled rice-bran Garima Bhardwaj1‚ Swaranjit Singh Cameotra2‚ Harish Kumar Chopra1* 1*Department of Chemistry‚ Sant Longowal Institute of Engineering and Technology‚ Longowal - 148106‚ Distt. Sangrur (Pb.)‚ India. E-mail: hk67@rediffmail.com Fax: 91-1672-280072; Tel: 91-1672-305204 (O) 2Institute of Microbial Technology‚ Sector-39-A‚ Chandigarh-160036‚ India Abstract: Pseudomonas cepacia MTCC 7099 was studied for the biosurfactant
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Identification of Unknown Carbohydrates using various biochemical tests Date of experiment: 11/10/2012 Name of Student: Vicki Marshall Introduction A series of biochemical tests was carried out to identify unknown carbohydrates. There were six carbohydrates that needed to be identified; they have been randomly labelled A-F. The carbohydrates are glucose‚ fructose‚ maltose‚ lactose‚ sucrose and starch. There was six tests that were carried out to help identify them‚ these were: Iodine Test
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A Report of Experiment 1 Purification and Identification of An Unknown Mixture Aim To separate a mixture of a neutral (ester‚ alcohol‚ ketone‚ aldehyde or ether) and an acidic compound (phenol or carboxylic acid) or a neutral and a basic compound (amine)‚ and to identify the unknown mixture by physical (M.P. or B.P.) and spectroscopic methods (NMR‚ IR and MS). Method The mixture (sample number: 27) was dissolved in 200 mL of CH2Cl2 and then extracted with 10% NaOH. The acid was removed
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