Identification of A Mixed Culture Unknown An experiment such as this one serves the purpose of allowing us‚ the students‚ to apply what we already know about any organism and any laboratory procedure to the difficult task at hand. It is possible to identify a mixed culture by running familiar experiments on the unknown bacteria and taking information already known about specific bacteria and applying it to the results. This helps to slowly eliminate any bacteria that do not correspond with the
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yeasts‚ pink yeasts Rhodotorula Fresh meat Putrefaction Alcaligenes Clostridium Proteus vulgaris Pseudomonas fluorescens Cured meat Mouldy Aspergillus Rhizopus Penicillium Souring Pseudomonas Micrococcus Greening‚ slime Lactobacillus Leuconostoc Fish Discoloration Pseudomonas Putrefaction Alcaligenes Flavobacterium Eggs Green rot P. fluorescens Colorless rots Pseudomonas Alcaligenes Black rots Proteus Concentrated orange juice "Off" flavor Lactobacillus Leuconostoc Acetobacter Poultry
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HOW TO WRITE AN UNKNOWN LAB REPORT IN MICROBIOLOGY GENERAL Unknown reports in microbiology are written in scientific format. Scientific writing is written differently from other types of writing. The results of the exercise or experiment are what are being showcased‚ not the writing. The purpose of scientific writing is not to entertain‚ but to inform. The writing should be simple and easy to understand. There is a specific style that must be followed when writing scientific reports.
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or become solid due to clot formation Organism Result + or - Appearance/Observations Staphylococcus aureus Staphylococcous epidermidis D. Urease Acitvity: identification of Proteus spp. Note: the color of the urease slant Organism Result + or - Appearance/Observations Proteus vulgaris + Bright pink in color Escherichia coli - Yellow in color E. Hemolysins Note: the appearance of the colonies and/or media. Organism Result
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Weekly iLab iLab: Exploring the Microbiology Lab Section 1 Lab Safety 1. There are four safety equipment items that a lab should have. Identify two of these four items. (2 pts) Biological Safety Cabinet‚ Eyewash and shower 2. Identify one of the three ways to keep your work area safe. (1 pt) Keep your workspace free of all unnecessary materials 3. There are five recommendations for dressing properly in a lab environment. Name two of these recommendations. (2 pt) Avoid loose
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change to the media will impart a blue color throughout the media or cause a blue band to form at atop of the media. This PH change is a result of proteolytic and degradative enzymes producing ammonia or amines form the breakdown of proteins. * Proteus
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Pure Culture Techniques In this first lab‚ you will be learning some very fundamental and important techniques. As is the case with most things‚ shorts cuts usually get you in trouble. This is especially true in Microbiology. The techniques you will be learning tonight‚ if mastered correctly‚ will make your life and learning experience in Microbiology much easier‚ if you don’t pay attention and practice these techniques incorrectly‚ well then……? Today you will be learning the following techniques:
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(prodigiosin: non water soluble) and LIA is P/P ( purple slant and butt) ------ S. marcecens --------------> if LIA is is P/Y (purple slant with yellow butt) --------- S. paratyphi A *TSI: ALK/AG w/ H2S ---- Proteus vulgaris‚ Salmonella typhimurium -->TSI butt and slant all black in color (rightmost tube) [pic] if LIA is R/Y (red slant‚ yellow butt) ----- P.vulgaris and PAD+ if LIA is P/P (purple slant and butt) ------- S. typhimuriuM
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tests indicated that the unknown #4 was Streptococcus pyogenes. All seven tests on the unknown matched S. pyogenes perfectly. The blood agar plate proved the unknown to be β hemolytic‚ meaning the unknown bacteria was capable of complete hemolysis. This test separated the unknown into the β Streptococcus group‚ narrowing the possible bacteria to S. aureus or S. pyogenes. The Catalase test was used to determine if the unknown could break down hydrogen peroxide. The unknown bacteria was unable to break
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Makati Medical Center College of Nursing DRUG STUDY Patient’s Name: M.D.C. Age: 42 y/o Medical Diagnosis: Leptospirosis Attending Physician/s: M.T Allergies: none Allergic Responses: none Prepared by: Shiela Guiquing |DRUG NAME |MECHANISM OF ACTION |RATIONALE FOR THIS PATIENT |ADVERSE DRUG EFFECT |NURSING IMPLICATIONS | |
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