2.3. Method Development 2.3.1. Preparation of Stock Solution 100 mg of the synthesized compound was made up to 100 ml into a 100ml standard flask with ethanol to give a solution of 1000 μg/ml. 2.3.2. Determination of λ max 1 ml of stock solution was pipetted out was made up to 10 ml into a 10ml standard flask with ethanol to obtain strength 100 μg/ml and scanned at 200-400nm using a UV spectrophotome-ter (Deepak V Bageshwar et al.‚ 2010). 2.3.3. Preparation of Standard Calibration Curve Aliquots
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04/29/2013 G-Unknown code Gram Positive-Micrococcus luteus Gram Negative- Klebsiella pneumoniae Unknown Paper The unknown project was a very good realization of me and my partner going out by our selves. The very first day of the Unknown project I was acquainted with Denise who was very friendly and was very nice as far as assisting me in the project. The first day we were introduced too various different forms of the unknown such as broth‚ Blood agar plate‚ MSA plate
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Having the ability to collect and identify unknown microorganisms is vital in health and medicine. This capability is important for a variety of reasons‚ such as knowing the causative agent of disease‚ knowing if the microorganism obtains any beneficial properties and knowing the correct microorganism to use to create a successful antibiotic. Implementing the experimental methods learned thus far in the microbiology laboratory allowed an unknown bacterium to be identified as a result of this study
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this lab was to determine the density of water and an unknown liquid. Density is defined as the mass of a substance divided by its volume. It is an intrinsive property of matter and is used to specifically characterize substances. III. Procedure: 1.We massed an empty 10mL graduated cylinder to the nearest .01g. 2.Then‚ we filled the graduated cylinder with 4.0-5.0 mL of distilled water‚ 3.massed it to the nearest 0.1 ml and 4. recorded the data. Then to determine the density of the unknown liquid;
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The purpose of this report is to explain the process and steps used to identify a certain microorganism referred to as the unknown. Materials and Methods A test tube with an unknown microorganism will be retrieved. Once the test tube is retrieved‚ a steak for isolation will be completed in order to produce isolated colonies of the organism on an agar plate. The unknown test tube with the bacteria is flame sterilized using a Bunsen burner. Once the bacterium test tube has been flame sterilized
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by aseptically inoculating a tube of phenol red sucrose broth‚ and a tube of phenol red arabinose broth with the unknown culture and incubating at 37 degrees Celsius for 48 hours. After incubation‚ the two tubes were examined for color change. The eighth test was to find out if the bacteria in question had flagella. The motility test was performed by aseptically inoculating the unknown bacteria into a tube of TSA broth and allowing it to incubate for 24 hours at 37 degrees Celsius. After the TSA
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The Unknown Citizen "The Unknown Citizen" by W.H. Auden‚ is a commentary on government and the materialism of modern man. The poem is written in the form of an obituary inscribed on a monument built by the government in commemoration of an average‚ upstanding‚ and decent community member. Throughout the passage‚ the speaker lists facts about the citizen’s life which he believes prove that the deceased was a valuable person. In actuality these facts represent nothing more than the socially accepted
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An Unknown Girl In the poem ‘An Unknown Girl’‚ Moniza Alvi uses poetic techniques such as metaphors‚ personification‚ alliteration‚ repetition and similes in order to depict her struggles in rediscovering her cultural identity. Along with references to India and the scenery surrounding the narrator‚
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Analysis of an Unknown Mixture Performed: 2/13/14 Submitted: 3/3/14 Abstract: The purpose of this experiment was to separate and identify the two unknown compounds. The sample used was 105 and it contain 2-propanol and 2-heptanone. IR spectrums were taken of the contents of the second and sixth test tubes and showed the presence of an OH functional group and a small amount of ketone in test tube two and a large presence of ketone in test tube six. The average boiling points of the test tubes
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Business Plan Materials Department Hospitality Operations Management Stenden University – International Hospitality Management Leeuwarden The Netherlands Submitted in Partial Fulfilment for the Requirements of the degree Programme Bachelor of Business Administration (BBA) in International Hospitality Management. April 2011‚ Module 3 Materials Department Odd weeks Nick Sweertman - 79125 Even weeks Susanne Dupuis -117579 Declaration of own work 1. This work
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