Macromolecules Lab Purpose: to determine a method of testing for macromolecules. Materials: Knowns: Unknown: Test Solutions: Glucose solution Unknown solution Iodine solution Gelatin solution Benedict’s solution Starch solution Biuret solution Oil Brown paper Water Procedure: 1. Create a data table. 2. Label 5 test tubes with known solutions. 3. Add 10-20 drops of each known solution to respective test tubes‚ do not mix pipets! 4. Add 3-5 drops of
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This experiment tested the growth of E.coli with inserted plasmid on an agar plate with Ampicillin. One colony of E.coli resistant to Ampicillin was grown during this experiment. The overall goal of the experiment was to successfully grow E.coli on the agar plate‚ which would show that the plasmid had been effectively inserted into the bacteria’s genes. This experiment helped students understand how plasmids were inserted into bacteria and used in real life situations. It also showed how the bacteria’s
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Conclusion: (Identity‚ % error‚ other evidence‚ how to improve) The identity of Unknown A is the compound cobalt(II) chloride hexahydrate (CoCl₂•6H₂O).This is justified by the similarities in color of red-purple crystal like grains‚ which is significant in identifying the product out of the eight suggested identities. Only two had similar characteristics which were CoCl₂•6H₂O and CoSO₄•6H₂O. Before unknown A was heated‚ there was a notable common physical property of the substance being red crystal
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Purpose: Unknown mixtures will be separated by means of chromatography in which the mixture will be passed in a solution through a medium leaving behind components of the mixture at different rates‚ therefore‚ different spots on the absorbing substance. This will help determine the identity of unknown mixtures. The spot colors on the strip of filter paper and the Rf values of the unknown samples will be compared to those of known samples. To find the position of the spots on the strip of paper‚ we
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The Escherichia Coli bacterium was discovered in 1885 by bacteriologist Theodor Escherich. He found the bacteria in the human colon and showed that some strains of Escherichia Coli were responsible for infant diarrhea and gastroenteritis. The Escherichia Coli was originally named Bacterium Coli. The name was changed to honor Theodor Escherich. E coli are normally harmless and are part of the normal flora found in the lower intestines of humans and healthy cattle. Some E. coli serotypes are known
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The first test conducted on unknown bacteria 32 was the Gram stain. From this stain‚ unknown 32 was found to be a Gram-positive cocci. This test eliminated all possible Gram-negative bacteria‚ Gram-positive rods and Gram-positive spirillium. Next‚ the endospore test determined whether or not the Gram-positive bacteria contained endospores. With the use of malachite green‚ steam‚ and safranin it was found that unknown bacteria 32 did not contain endospores. This eliminated Gram-positive cocci Sporosarcina
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observing and theorizing a possible correlation between the decline of sales of the restaurant’s beef products and the increase of reporting of E. Coli contamination at local grocery stores. It will be imperative for the ownership to take up on this research proposal‚ because if the findings result in the fact that there is a possible correlation between the reports and the decline in sales‚ the ownership will possess the necessary information to make substantial
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E. coli O157H7 What is the morphology and gram reaction of this pathogen (2) E. coli‚ including E. coli O157H7 is a gram-negative bacillus. What do (i) O157 and (ii) H7 attached to the name of this bacterium represent (2). The O157 is the O HYPERLINK http//www.emedicinehealth.com/script/main/art.asparticlekey5469 serotype antigen that identifies the E. coli strain‚ and the H7 represents the antigen type on the HYPERLINK http//www.emedicinehealth.com/script/main/art.asparticlekey2416 bacteriums
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Escherichia coli also known as E. Coli‚ is a type of bacteria which thrives in polluted water. Many people will come in contact with this type of bacteria in their lifetime. In fact‚ E. coli is normally found in the intestine of humans and animal where it plays a significant role in the digestive system. The bacteria can also be found in raw meat and uncooked vegetables as well as polluted water. Most of the strain of E. coli are harmless therefore they will not cause illness if consumed. “One of
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In the experiment involving varying pH levels‚ E. faecalis‚ S. epidermidis‚ L. lactis‚ and L. casei were subjected to different pH levels and then were allowed to incubate in order to determine the minimum‚ maximum‚ and optimum pH levels for growth of specific bacterial species. It was found that lower pH levels between 2 and 4 inhibited or promoted little to no growth for E. faecalis‚ S. epidermidis‚ and L. casei. It is evident that these bacteria were resistant to alkaline environments‚ however
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