1) TITLE (2pt): Testing antimicrobial props in soaps 2) INTRODUCTION (6 pts): In this experiment we wanted to test how effective different types of soaps against E. Coli. For this experiment we were interested in looking at antimicrobial soaps vs. non-antibiotics soaps in their effectiveness to kill E. Coli. The protocol that we used was the Kirby Bauer technique. This technique was used to test the antimicrobial properties of each soaps. (“Kirby-Bauer” 2015). 3) METHODS (6 pts): We first started
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This article is about public and private swimming pools being contaminated with E. coli. Chlorine is not doing its job in sanitizing the human waste in the swimming pool. Human waste seems to refuse being sanitized. The Centers for Disease Control (CDC) concluded that the E. coli came from one major primary source; swimmers pooping in the swimming pool. Researchers say such contamination is due to swimmers not rinsing themselves off before going in the pool. Scientists say a person carries about
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Identifying an Unknown Aldehyde or Ketone Introduction The purpose of this lab is to identify an unknown aldehyde or ketone substance using chemical tests. The chemical tests used in this experiment are solubility‚ Schiff‚ Bisulfite‚ and Iodoform tests. Also‚ a 2‚4-dinitrophenylhydrazone derivative synthesis reaction will be completed from which a melting point will be obtained. The chemical test results and the melting point analysis will be compared to the table of compounds given to find the
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METHODS In this experiment‚ plasmid lux and a control plasmid (pUC18) will be introduced into E. coli by transformation. There are four basic steps to the procedure; Preparation of competent cells (These steps should be performed by the instructor.) 1. Place a vial of CaCl2 solution and the tube of E. coli in the ice bath. 2. Using a sterile pipet‚ transfer 590µL CaCl2 solution to the tube containing 50µL of the bacteria. 3. Tap the vial with the tip of your index finger to mix the solution. 4. Incubate
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their food intake is through meat and dairy products and it’s not a lie when people say the food industry is a bunch of shady people. Past generations have been able to eat raw beef without even considering consequences like e-coli‚ but at this point in the United States e coli is the least of our worries. It is time to stand up against the food industry and make a change for the better of our world‚ and our future generations. The United States is a country where food is being mass-produced. We
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performed this test‚ the initially slant for unknown microorganism #17 is important. For this lab‚ two identical slants are used for two reasons. Firstly‚ the slant can be used to make sure that there is no contamination from the Nutrient Agar plate. Secondly‚ the second slant will become a stock culture to prevent the shortage of slants during performing the series of tests. Kliger’s Iron Agar tests can be used to determine multiple characteristics of unknown microorganism #17. Kliger’s Iron Agar slants
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Introduction The purpose of this lab was to explore the properties of an unknown compound. An unknown was given and a cation flame test and anion test was performed to determine the identity of the compound. Once the identity was determined‚ the properties were explored. Experimental To determine the cation of the compound‚ a cation flame test was performed. A bunsen burner was lit until a medium blue flame was burning. The given unknown was scooped onto a nichrome wire loop. The wire was held
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Main text. Questions. Page 3. 1. What does e.coli stand for? E.coli stands for Escherichia coli. 2. What are the symptoms associated with E.coli bacterial infection? The symptoms are diarrhea‚pain‚cramping‚nausea and vomiting. 3. give a detailed comparison of the levels of the e.coli bacteria over the five week period as given above. Page 4. 4. What could possible reasons
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Unknown 2 Scheme “ Fair game” ions Cations: Na+‚ K+‚ NH4+ ‚ Ca2+‚ Mg(OH2)62+ ‚ Al(OH2)63+ Anions: SO42-‚ HSO4-‚ NO3-‚ OH-‚ Cl-‚ CO32-‚ HCO3- Insoluble Compounds: Ca(OH)2‚ CaSO4•2H2O‚ CaCO3‚ MgCO3‚ Mg(OH)2 I. Describe Sample a. Quick description of sample. Ex: Phase‚ color‚ odor‚ crystalline‚ amorphous‚ gel-like‚ powdery‚ etc. II. Flame Test a. Part 1 i. Orange Flame: Na+ is present. K+ ‚ NH4+ ‚ Ca2+‚ Mg(OH2)62 ‚ Al(OH2)63+ may also be present. ii. Purple Flame: K+ is present (no Na+
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Requirements for Unknown Report You have been provided a mixed culture (a gram positive and a gram negative) from which you should have isolated each type of organism‚ described their colonies on a TSA plate‚ and described their cellular morphology. You were graded on this activity separately. After you have isolated your gram negative in pure culture you will be subjecting it to various biochemical tests and making observations about the cells‚ colonies‚ growth temperature preferences‚ etc. in
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