Molecular Biology Lab Report Payton Jackson Introduction In this lab‚ I am going to use antibiotic-resistance plasmids to transform Escherichia coli. Materials For this lab you will need the following: LB Agar Petri dishes Beakers Test tubes CaCl2 solution Sensitive E. coli (-ampR) amp plasmids ampicillin -amp cells Water bath to heat shock cells A freezer to incubate cells Process Step 1: Wash hands and sanitize lab setting. This will prevent anything reacting with a
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purpose of the unknown bacteria lab assignment was to select an unknown bacteria culture and‚ through a series of metabolic tests‚ identify which bacteria genus resided in the pure culture received. A nutrient broth inoculated with bacterial culture (numbered 45‚ henceforth referenced as U45) was selected and a streak plate was made to isolate a pure culture for use throughout the assignment. From the streak plate‚ several slides were made to determine the morphology of unknown 45. A Gram stain‚ used
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Biology 205 MW 2:10pm. Unknown Organism Paper The unknown organism tested in the Mesa lab was collected from in between the toes of my puppy‚ Riley. The original culture grown consisted of dozens of visibly different organisms of varying colony colors and growth patterns. I chose to test a smaller‚ red colony for my experiments. Based on its gram reaction and oxygen requirements‚ the following tests were performed to reach my presumptive ID: Test Performed Result Gram Stain + Fluid Thioglycollate
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1. Obtain 1 gram of an impure‚ unknown substance solid. Make sure to stir the mixture before measuring the sample; record the mixture’s code in the data section. 2. Add approx. 25-50 mL of water and several boiling chips to a 125 mL Erlenmeyer flask and heat the water to a gentle boil using a hotplate. 3. On a second hotplate‚ place a 125 mL Erlenmeyer flask containing the one gram of unknown solid along with a boiling chip. 4. Using a ring clamp‚ slowly pour approx. 5-10 mL of boiling water into
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their abilities to develop and adjust to altering environments. That is why it is so significant to be competent to identify microorganisms. This study was conducted by applying techniques and experiments cultured in microbiology lab for the identification of the unknown microorganism. Introduction: Identification of bacteria is significant because they are not only beneficial but potentially hazardous as well. The identification of bacteria can lead to breakthroughs in healthcare regarding treatment
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The purpose of the following study is to determine where the two unknown bacteria acquired in Microbiology lab should be classified in regards to temperature‚ pH level‚ and osmoregularity. It is important to classify bacteria in order to identify them. Identification of bacteria is important because they are not only useful but potentially dangerous as well. The identification of bacteria can lead to breakthroughs in healthcare regarding treatment of old and new diseases alike. Identifying bacteria
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Micro Biology Lab Gram Staining Lab Report Purpose The purpose of this lab was to experiment with a staining technique called Gram Staining. Gram Stains is used to identify and classify bacteria‚ it is a differential stain that allows you to classify bacteria as either gram-positive or gram-negative. The foloowing is a breif summerary of the gram staining procedure. Apply the primary stain Apply Mordant Apply decolorizing agent Apply the secondary stain Bacteria that
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Microbiology Laboratory Report Identification of Unknown Bacteria 03/10/05- 04/01/05 Authors: Richard Hendricks‚ Jessica Prebish; NMU Abstract: Broth culture 16 was randomly selected by our group and subjected to qualitative tests for taxonomic identification. The culture did appear homogenous throughout the testing period and is currently retained by Northern Michigan University’s department of Microbiology. We suggest that culture 16 is an example of Escherichia coli. Background: Techniques
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Identification and Diagnosis of Unknown Enteric Bacteria in an Infected Patient Using an Enteropluritube Brooke Addario 2/16/2015 BIO 488C - Section J TA – Jacob Karsten Introduction: Enteric bacteria are members of the Enterobacteriaceae family of microorganisms. These gram negative‚ rod shaped‚ facultative anaerobes are found in the intestinal tracts of humans and animals. Enteric bacteria can be pathogenic; however‚ they are also a normal part of human bacterial flora and are therefore
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Microbiology-2460 Lab-003 March 31‚2008 Lab Report-Escherichia coli Abstract The purpose for this lab report was to identify and inform of an unknown bacteria that has been causing a patient to have lower abdominal and pelvic pain. To obtain the identification of this unknown bacterium‚ several biochemical tests needed to be performed in order to prescribe the correct medication to treat and cure the symptoms. Introduction In a lab today‚ I am to identify an unknown bacterium that is causing
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