Transformation of E. coli by plasmid DNA 1. Table showing the results from the selective plates |Plate |Plasmid |Contents of plates |Number of colony | | | | |White |Blue | |1 |Ligation mixture |Ampiclillin + X- gal + IPTG |10 |0
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Research Question How will the addition of different pH buffers to amylase affect the rate of starch digestion measured using starch and iodine? Introduction Amylase is an enzyme found in human saliva and pancreas. It is the digestive enzyme that is needed to breakdown starch molecules. Amylase must be kept at certain conditions to function at its optimum level. This experiment will explore the effect of pH (1‚ 4‚ 7‚ 10‚ and 14) on the function of amylase by using starch and iodine. Usually
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Determination of presence of protein in a variety of food substances by the use of Biuret Reagent Biol. 1040 Section 1005 Introduction The human body consumes carbohydrates‚ lipids‚ and proteins each day by eating a variety of foods. These foods are required for energy and human growth. Proteins are a diverse group of macromolecules with many different functions (Mbuthia‚ 2012). Many are structural components of bone‚ muscle‚ hair‚ tissue‚ etc. Others are enzymes that speed up cellular
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Page 1/3 09/2010 SE 110.22 Forces in an Overdeterminate Truss The picture shows SE 110.22 in a frame similar to SE 112. * Comparison of forces in statically determinate and overdeterminate trusses1 * Bars with strain gauge full bridges to measure bar force1 * Computerised evaluation of experiments Technical Description Overdeterminate trusses are employed where overdimensioning is purposely required because safety must be maintained in the event of failure of an element‚ such as in aircraft
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Introduction The natural environment is highly populated with microorganisms such as bacteria‚ fungi‚ protista and several others. Microorganisms are microscopic in size and vary shape. They are found in the air‚ in water‚ on land and on other organisms such as humans and pets. Many microorganisms grow at rapid speeds and can be harmful and cause diseases. Although microorganisms may cause harm‚ society benefits from microorganisms in many ways. Microorganisms are necessary for the production of
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diffusion‚ however‚ deals solely with water. Osmotic pressure is the pressure of a solution against a semi-permeable membrane to prevent water from flowing into the membrane. In this lab‚ we are going to study tonicity; tonicity is the measure of this osmotic pressure and is the differential of pressure between two solutions separated by a selective membrane. To help identify the relative concentrations of solute particles of different solutions‚ we must understand that there are three possible differences
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Calorimetry Lab Report Waris Butt PHY 112 Mr. Fasciano Class #18336 06/08/14 Purpose: Heat flow will occur between objects in contact until no more heat flow is detectable. Using calorimetry to analyze heat flow quantitatively and the equation: Q = mc ΔT‚ to determine the specific heat capacity of an object and heat flow from or to an object; respectively. Materials: Circle K 44 oz Styrofoam cup with lid Large Plastic
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Cheese: A Home to Bacteria “Cheese is just a means of controlled spoilage‚” said Steve Jenkins‚ a Master Cheesemonger at Fairway Market. (Cheese) Bacteria‚ or starter cultures‚ control the flavor‚ hardness‚ type‚ and many more aspects of cheese. The two main strands of bacteria involved in cheese making are Lactococcus and Lactobacillus‚ which ferment sugars found in the milk. Humans have learned to manipulate bacteria and the fermentation process to produce different types of cheese such as Swiss
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decrease in pH‚ more bacteria present‚ and an increase in absorbency. The results proved that fermentation occurred with three positive confirmatory results from the gas production‚ pH‚ and turbidity tests‚ while the Gram stain test acted as a negative confirmatory test. Introduction: The main goal of this experiment was to successfully ferment milk into a yogurt-like product called Kefir. The fermentation is accomplished with a symbiotic combination of different bacteria and yeasts. The main type
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not exist (Lab Manual 3 pg. 1). They help in many different ways that are useful to the body of living organisms. Enzyme are used to speed up chemical reactions (Lab Manual 3 pg. 1). Through this process‚ they are considered very unique because they are not altered or consumed within the reaction (Lab Manual 3 pg. 1). This is why enzymes are considered biological catalysts. They also do not alter the equilibrium of a chemical reaction nor the amount of free energy that is released (Lab Manual 3 pg
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