Introduction: The gram stain is one of several laboratory procedures that can be used to narrow down the identities of unknown bacteria. Bacteria have three different shapes; cocci‚ bacilli‚ and spirilla. Since bacteria pretty much have the same reflective index as water‚ a bacteria cell must be dyed so that these shapes can be seen. Materials: Petri dish Dropper Cleansing solution Slides Bibulous paper Inoculation loop Crystal violet dye‚ Iodine Acetone Safranin Water from the sink
Premium Staining Gram staining Microbiology
Margaret E Gibson July 20‚ 2009 Microbiology Dr. Metera Lab Report 3: Labs 7 and 8- Metabolism and Biochemical Tests Abstract This experiment focused on metabolism and biochemical tests. The goal of performing these tests was to differentiate microbes from one another and to compare how metabolic and biochemical processes differ from species to species. The tests performed include: the Fermentation of Sugars Test (sucrose‚ glucose‚ and lactose)‚ the Urease Test‚ the Fermentation
Premium
Macromolecules Lab Purpose: to determine a method of testing for macromolecules. Materials: Knowns: Unknown: Test Solutions: Glucose solution Unknown solution Iodine solution Gelatin solution Benedict’s solution Starch solution Biuret solution Oil Brown paper Water Procedure: 1. Create a data table. 2. Label 5 test tubes with known solutions. 3. Add 10-20 drops of each known solution to respective test tubes‚ do not mix pipets! 4. Add 3-5 drops of
Premium Chemistry Bacteria Experiment
Introduction The purpose of this experiment is to see how much bacteria can and will grow in common places in a typical high school. Common places can mean many things‚ including anything from water fountain spouts to computer keyboards. Such objects can hold up to 2‚700‚000 colony forming units per square inch (or CFU/in sq)(NSF). A colony forming unit is the unit used to find an estimate of the number of cells of a bacteria. This unit of measurement is commonly utilized in the subject of microbiology
Premium Bacteria Agar plate
Conclusion: (Identity‚ % error‚ other evidence‚ how to improve) The identity of Unknown A is the compound cobalt(II) chloride hexahydrate (CoCl₂•6H₂O).This is justified by the similarities in color of red-purple crystal like grains‚ which is significant in identifying the product out of the eight suggested identities. Only two had similar characteristics which were CoCl₂•6H₂O and CoSO₄•6H₂O. Before unknown A was heated‚ there was a notable common physical property of the substance being red crystal
Premium Chemistry Chemical substance Ion
Purpose: Unknown mixtures will be separated by means of chromatography in which the mixture will be passed in a solution through a medium leaving behind components of the mixture at different rates‚ therefore‚ different spots on the absorbing substance. This will help determine the identity of unknown mixtures. The spot colors on the strip of filter paper and the Rf values of the unknown samples will be compared to those of known samples. To find the position of the spots on the strip of paper‚ we
Premium Chemistry Chromatography Chemical substance
Judson Cotterman III 4/6/2012 Section 185-35L Assignment #3 Gram-positive Pathogenic Cocci Discussion My original hypothesis for the individually collected throat culture on Blood Agar Plates‚ that it would show a mixture of alpha‚ beta‚ and gamma hemolysis‚ was based on the results of the class control samples of S. pneumonia‚ S. aureas and S. epidermis. In these controls S. pneumonia shows alpha and beta hemolysis while S. aureas and S. epidermis showed beta and gamma hemolysis respectively.
Premium Bacteria
this experiment which are the brand of dishwashing detergent; Palmolive‚ Morning Fresh and Ultra Coles Brand and the type of bacteria used which are; Micrococcus Luteus and an unknown bacteria from a sponge. Dependent Variable The dependent variable is the factor that is being tested which is the size of the zone of inhibition from the detergent solution and the bacteria. It will be measured in millimetres using a ruler. Constant Variables Constant Variables are set in place in order to produce
Premium Water Mathematics Experiment
organism we had in our unknown mixed culture tube by running a series of experiments to detect which specific Gram negative organism we had. To detect your gram positive from the mixed culture was given as extra credit points also. A Gram stain was performed and isolation streak plate in order to isolate and observe the unknown organism. Before the series of test‚ a dichotomous key had to be written up in order to know what steps and tests to run to identify the unknown Gram negative organism. I had
Premium Bacteria Microbiology Staining
Introduction The purpose of this lab was to explore the properties of an unknown compound. An unknown was given and a cation flame test and anion test was performed to determine the identity of the compound. Once the identity was determined‚ the properties were explored. Experimental To determine the cation of the compound‚ a cation flame test was performed. A bunsen burner was lit until a medium blue flame was burning. The given unknown was scooped onto a nichrome wire loop. The wire was held
Premium Sodium chloride Sodium