an Unknown Compound Purpose: The purpose of this experiment was to separate a mixture of two or more unknown compounds and identify them based on their melting point and reactivity with an acid or a base. Compound studied/Reactions: The organic solvent used in this experiment was methyl t-butyl ether (MTBE‚ 2-methoxy-2-methylpropane). The unknown that dissolved in the MTBE‚ but was insoluble in water. MBTE is very volatile and flammable. It was determined in the experiment that the unknown was
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During my time at the University of Wisconsin- La Crosse‚ it was there that I not only discovered my passion for microbiology; but it was where I became aware of my career goal‚ to be an educator at the university level. I am grateful for the many experiences and the motivation brought on by my peers and mentors‚ which I came across to realize and to bring to fruition my goals. I strongly believe that the education that I receive in conjuncture with the strong mentorship that the State University
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FOOD AND INDUSTRIAL MICROBIOLOGY Food spoilage‚ food infections and intoxications caused by microorganisms and methods for their detection Dr. Neeraj Dilbaghi Reader‚ Department of Bio & Nano Technology Guru Jambheshwar University of Science and Technology Hisar- 125001 and Dr (Mrs.) S. Sharma Professor‚ Department of Microbiology‚ CCS Haryana Agricultural University‚ Hisar- 125001 (Revised 25-Sep-2007) CONTENTS Introduction Food Spoilage and General Principles Underlying Spoilage of Food Intrinsic
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A Basic Understanding of Microscopy Assignment 1 Kaplan University-Microbiology Since microorganisms are not visible to the eye‚ the essential tool in microbiology is the microscope. One of the first to use a microscope to observe microorganisms was Robert Hooke‚ the English biologist who observed algae and fungi in the 1660s. In the 1670s‚ “Anton van Leeuwenhoek‚ a Dutch merchant‚ constructed a number of simple microscopes and observed details of numerous forms of protozoa‚ fungi‚ and bacteria”
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Identification and Diagnosis of Unknown Enteric Bacteria in an Infected Patient Using an Enteropluritube Brooke Addario 2/16/2015 BIO 488C - Section J TA – Jacob Karsten Introduction: Enteric bacteria are members of the Enterobacteriaceae family of microorganisms. These gram negative‚ rod shaped‚ facultative anaerobes are found in the intestinal tracts of humans and animals. Enteric bacteria can be pathogenic; however‚ they are also a normal part of human bacterial flora and are therefore
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Microbiology‚ Test 3 Study Guide Chapters 14‚ 15‚ 16 and 17 Chapter 14 This chapter is about the principles of disease and epidemiology. Epidemiology – the science that studies when and where diseases occur and how they are transmitted. CDC‚ Atlanta‚ tracks and traces diseases. ONE World (the idea that it’s all in one place‚ it could happen anywhere; locally‚ states‚ large counties track the incidences and occurrences of disease)
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bacteria. Those tiny living creatures are remarkably intricate. Witnessing the breathtaking phenomena created by marine microbes strengthened my determination to further pursue my study of marine microbiology. In my first year as an undergraduate student I participated in lab work on marine microbiology. I led one project on separation and identification of pigment-containing bacteria in the Xiamen Coastal Areas‚
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INTRODUCTION Total Viable Count is a quantitative idea about the presence of microorganisms such as bacteria‚ yeast and mold in a sample. It counts the number of colonies produced by a very dilute suspension of bacteria on an agar plate and to observe the differential staining behaviour of the living bacteria. This involves counting the colonies produced by viable cells under favourable growth conditions. Some techniques needed before the viable count‚ like pour plate method‚ spread plate method
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Requirements for Unknown Report You have been provided a mixed culture (a gram positive and a gram negative) from which you should have isolated each type of organism‚ described their colonies on a TSA plate‚ and described their cellular morphology. You were graded on this activity separately. After you have isolated your gram negative in pure culture you will be subjecting it to various biochemical tests and making observations about the cells‚ colonies‚ growth temperature preferences‚ etc. in
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spectrometry. We will do this by making 2‚ 4 a DNPH derivative and checking the melting points. Theory: By Using specific methods of compounds detection‚ we can match an unknown compound with a known compound because similar compounds will display similar characteristics. In this experiment‚ identifications of the unknown ketone was accomplished using thin layer chromatography‚ melting point‚ and NMR spectrometry. Thin layer chromatography is very quick but sensitive way of determining the components
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