Requirements for Unknown Report You have been provided a mixed culture (a gram positive and a gram negative) from which you should have isolated each type of organism‚ described their colonies on a TSA plate‚ and described their cellular morphology. You were graded on this activity separately. After you have isolated your gram negative in pure culture you will be subjecting it to various biochemical tests and making observations about the cells‚ colonies‚ growth temperature preferences‚ etc. in
Premium Escherichia coli Gram staining
General information: What is E.coli? Escherichia coli‚ or commonly referred to as E.coli is a foodborne pathogen that normally inhabits in the intestinal track of humans and animals. It is the most common and wide spread infecting organism and is in the family‚ Enterobacteriaceae. Enterobacteriaceae is a large family of Gram-negative bacteria‚ meaning the bacteria appear pinkish or red. How fast and how does it spread? The main way that E.coli can spread is through food and water supply. Common
Premium Bacteria Immune system Microbiology
which organism we had in our unknown mixed culture tube by running a series of experiments to detect which specific Gram negative organism we had. To detect your gram positive from the mixed culture was given as extra credit points also. A Gram stain was performed and isolation streak plate in order to isolate and observe the unknown organism. Before the series of test‚ a dichotomous key had to be written up in order to know what steps and tests to run to identify the unknown Gram negative organism. I
Premium Bacteria Microbiology Staining
alteration of a cell‚ resulting from the intake of exogenous genetic material through the surrounding cell membrane. The purpose of this lab was to determine transformation of bacteria by testing the effect of P Vib plasmid of E. coli MM294‚ and how the color of the E. coli bacteria changes. In this lab‚ two small test tubes were given calcium chloride‚ E. coli MM294‚ and one of the tubes also received the plasmid P Vib. The test tubes were then placed in ice‚ heat shocked‚ and then a small amount
Premium
Microbiology Final exam Study reference 1. Gram Stain * Verify if bacteria are present or not. * Controls – positive (purple) – S.aureus negative ( red/pink) – E.coli 2. Endospore Stain Positive controller – B. magneterium Green spore- Positive Pink (vegetative ) – Negative 3. Acid fast Positive control – M. smeagmatis Blue – negative Pink /red- positive 4. Motility Positive control - P.vulgaris A. Non motile is negative test B. Motile is a positive test 5. Carbohydrates
Premium Gram staining Enzyme Bacteria
The unknown soda ash from experiment 3 was used‚ to determine the weight for each trial we used the equation of (M of HCl) x (18 ml x 105.99) / (10 x 2 x Na2CO3 ). Which was equal to (0.01472 M) x ((18 mL X 105.99)(10 x 2 X 2.428 % )= 0.6 g. To start we had to rinse the beakers‚ electrode and the stirring bar with diluted water. The sample we needed was weighted to the closest 0.1 mg which we got was 0.3 for the first trial. The sample was transferred to a 250 mL beaker and dissolved in 70 mL of
Premium Chemistry Mass Hydrogen
Laboratory Exercises in Microbiology‚ Fifth Edition Front Matter Preface © The McGraw−Hill Companies‚ 2002 PREFACE Take interest‚ I implore you‚ in those sacred dwellings which one designates by the expressive term: laboratories. Demand that they be multiplied‚ that they be adorned. These are the temples of the future—temples of well-being and of happiness. There it is that humanity grows greater‚ stronger‚ better. Louis Pasteur (French chemist‚ founder of microbiology‚ 1822–1895) There
Premium Microscope Microbiology Microorganism
as are their abilities to develop and adjust to altering environments. That is why it is so significant to be competent to identify microorganisms. This study was conducted by applying techniques and experiments cultured in microbiology lab for the identification of the unknown microorganism. Introduction: Identification of bacteria is significant because they are not only beneficial but potentially hazardous as well. The identification of bacteria can lead to breakthroughs in healthcare regarding
Premium Bacteria Microorganism Microbiology
by the emergence of Shiga toxin-producing Escherichia coli (Callaway et al.‚ 2013). Specifically‚ Shiga toxin-producing E. coli O157 is a foodborne pathogen of significant public health importance. It can cause mild to bloody diarrhea in humans which may progress to hemolytic uremic syndrome (Centers for Disease Control and Prevention‚ 1993; Hussein‚ 2007) that can be fatal in children‚ the elderly and immunocompromised individuals. E. coli O157 is also responsible for an estimated 63‚153 illnesses
Premium Bacteria Escherichia coli Microbiology
producing a form of stilbene (Figure 1). This reaction favored a crude Z-Stilbene crystal product over its E counterpart. When Z-Stilbene underwent photoisomerization with iodine for 1 hour it reconfigured almost exclusively into its more stable counterpart E-Stilbene. The reaction produced very low yield of 6.3% due to the nature of the reaction and the speed at which iodine reacts. The purity of E-Stilbene could have been increased by allowing the reaction to perform longer and to use a faster reactant
Premium Chemistry Ethanol Chemical reaction