LSM1102 Lab Report Introduction Transformation is a process which involves plasmid DNA being bound to the cell surface and the subsequent uptake of DNA by the cell (Panja et al.‚ 2008). For artificial transformation of E. coli cells with plasmids‚ plasmid DNA has to be extracted from bacterial cells using the High-Speed Plasmid Mini Kit‚ which is then mixed with competent E. coli cells followed by heat shock and the streaking of transformed cells on two different types of agar plate (LB and LB+ampicillin)
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-Unknown Mixtures Lab- Purpose: The aim of this experiment was to find out the two substances of the unknown mixture from salt‚ sugar‚ dirt‚ and baking soda by looking at its properties. Hypothesis: Find the unknown substance by testing it out by heating it‚ pouring water‚ vinegar‚ and observing it . Procedure: 1) Materials- * 100ml of water * Vinegar * Bunsen Burner * 2 beakers * 1 spatula 2) First‚ do steps (3-6)to find out the properties of salt‚ sugar
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Pure Culture Techniques In this first lab‚ you will be learning some very fundamental and important techniques. As is the case with most things‚ shorts cuts usually get you in trouble. This is especially true in Microbiology. The techniques you will be learning tonight‚ if mastered correctly‚ will make your life and learning experience in Microbiology much easier‚ if you don’t pay attention and practice these techniques incorrectly‚ well then……? Today you will be learning the following techniques:
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1 Student: ___________________________________________________________________________ 1. Extant microorganisms are organisms from the fossil record that are no longer present on Earth today. True False 2. All cellular organisms can be placed into one of three __________‚ which include the Bacteria‚ Archaea‚ and the Eukarya. ________________________________________ 3. Archaea are cellular organisms that have unique cell membrane __________. ________________________________________
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Osmosis 4/13/13 MLT1 Lab 9 Performed at Home Microbial Growth There are three types of environments in which cells are located which include isotonic‚ hypotonic and hypertonic. In an isotonic environment‚ the amount of water and solute are the same both inside and outside of the cell. As water drifts into the a cell‚ the same amount flows out creating a balanced environment both inside and outside of the cell. When there is a high level of
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classification ● Genus is represented by staphylococcus ● Designing organisms in the industrial setting that can provide human products through genetic engineering or clean up environment waste through bioremediation is part of the sub discipline microbiology referred to as biotechnology ● A pathogen is the term used to refer to any disease causing microorganism ● The term ubiquitous is used to refer to the fact that microbes exists nearly everywhere on the earth ● A good hypothesis must be: able to be supported or rejected by
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Lab Report Microbiology Introduction Bacteria can be found almost anywhere. For human life‚ some help us‚ some hurt us‚ and others are neutral. It is now known that good bacteria‚ or normal microflora‚ can reach 1014 microbial cells. This is far more than the 1013 cells that make up the human body (Tannock‚ 1995). The total number of bacteria on Earth is estimated to be around 4-6 x 1030 (Horner-Devine‚ 2004). It is important to know the extent of bacteria‚ how they live‚ and how they are
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specific epithet EX) Escherichia coli and Staphylococcus aureus are found in the human body. E. coli is found in the large intestine‚ and S. aureus is on skin Scientific names can describe an organism‚ honor a researcher‚ or identify the habitat of a species EX) Staphylococcus aureus - staphylo- describes the clustered arrangement of the cells; coccus indicates that they are shaped like spheres; aureus describes the golden-colored colonies EX) Escherichia coli – honors the discoverer Theodor Escherich
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wearing gloves 2: Appropriate for working with human body fluids. Autoclave‚ sharps containers‚ lab coats 3: appropriate for working with pathogens that can be transmitted via respiratory route. Self-closing‚ double doors and sealed windows 4: Highest level. Aerosol pathogens; pathogens with no vaccine/treatment. Separate building with separate ventilation and waste management systems Bacteria used in lab is classified as Class 1 by US Public Health Service/Biosafety Level 2 Review everything about
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ASEPTIC TECHNIQUES AND SOURCES OF MICROBIAL CONTAMINATION. Introduction The spread of infections has come to a point where it has become catastrophic. Aseptic technique is the method used to prevent contamination of infections. It is widely used in hospitals‚ pharmacy‚ and pharmaceutical industries and in laboratories. Different establishments have come up with more ways to improve infection control. In hospitals health care acquired infections are costing the NHS £1 Billion a year and
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