to stain everything other than the bacteria. The Gram stain is one of the oldest‚ most cost-efficient‚ yet most under-utilized‚ staining method used to identify bacteria. It consists of a basic dye (the primary stain crystal violet)‚ a mordant (Gram ’s iodine)‚ decolorizer (acetone:alcohol) and a counter stain (safranin). A mordant is a compound which helps hold the primary stain to the bacteria. In this case‚ the iodine forms a complex with the crystal violet to "lock it into" the cell wall/membrane
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A medical microbiology lab performs testing on human samples collected from different body sites. The tests are used to detect and identify any microorganisms capable of causing disease. Knowing of unknown microorganism is important on how this microorganism works and how it is structured‚ means knowing how it can affect humans. The purpose of this study was to identify an unknown bacterium by applying all methods that were previously conducted and learned in the medical microbiology laboratory
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HISTORY & PHYSICAL EXAMINATION REPORT Patient Name: T. J. Moreno Patient ID: 110497 DOB: 02/15/- - - - AGE: 44 SEX: M Room No.: 502 Date of Admission: 05/10/- - - - Admitting Physician: Patrick Keathley‚ MD‚ Endocrinology Chief Complaint: Left ankle pain. DETAILS OF PRESENT ILLNESS: This is a 44-year-old Hispanic male‚ when was kindly asked to admit by Dr. Max Hirsch the patient is status post arthrodesis of the left ankle and has newly diagnosed diabetes
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Introduction The purpose of this lab was to identify unknown bacteria cultures using various differential tests‚ and my unknown bacteria is #17. The identification of these unknown cultures was accomplished by separating and differentiating possible bacteria based on specific biochemical characteristics. Whether the tests performed identified specific enzymatic reactions or metabolic pathways‚ each was used in a way to help recognize those specifics and identify the unknown cultures. The differential tests
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Lab Report 2 Biological Media Meva Tinsley Georgia State University Lab Report 2 Introduction: Biological Media Microorganisms require nutrients and certain environmental conditions in order to thrive. In the lab‚ we use a Biological Media to aid in growth reproduction. Also referred to as a culture medium‚ a Biological Media is a substance used to support the growth of microorganisms. The two types of media most commonly used in Microbiology are selective media and differential
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Microbiology Laboratory Report Identification of Unknown Bacteria 6O BIO 251-7H1 Gram Stain Summary: Out of 28 Unknown microorganisms‚ slant 6O was randomly selected‚ and subjected to testing in order to identify it’s species. Seventeen of the unknown bacteria were classified as Gram-negative bacilli: Corynebacterium pseudodiphtheriticum‚ Enterobacter aerogenes‚ Eschirichia coli‚ Flavobacter capsulatum‚ Helicobacter pylori‚ Klebsiella pneumonia‚ Klebsiella oxytoca‚ Proteus hauseri‚ Proteus
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Preparation of SKEO The extraction of S. khuzistanica was performed by hydro distillation and clevenger apparatus for 5 hours. Once extraction was completed‚ the resulted essential oil was dehydrated using sodium sulfate (Merck). The essential oil was stored at temperature of 4ºC until using (12). In order to provide different concentrations of the extract‚ Dimethyl sulfoxide (DMSO) was used. Gas chromatography/mass spectrometry analysis of essential oil Isolation and measurement of the sample
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colorless colonies. Multiple medical experiments are credited towards transformation‚ especially ones involving cancer and the transformation of normal cells to neoplastic and cancerous cells. Introduction The general purpose of the Transformation Lab was to observe the difference in bacterial growth under differing media conditions to help understand the process of transformation and how it contributes to the life of living organisms. "Transformation" is what occurs when a cell receives and expresses
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Observations TISSUE TYPE OBSERVATIONS Simple Squamous single layer‚ flattened cells Simple Cuboidal single layered‚ cube shaped cells Simple Columnar (stomach) single layered‚ elongated cells Simple Columnar (duodenum) long columns in “S” shapes Stratified Squamous (keratinized) many layers‚ top cells flattened Stratified Squamous (non-keratinized) flat long strands with nucleus Pseudostratified Ciliated Columnar cells together in a column structure‚ single layer‚ elongated cells
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Alyse Rose Microbiology Lab Bacterial Unknown March 25‚ 2013 Bacterial Unknown Report Each student was given an unknown bacteria to figure out. I was given the unknown bacteria S38. Everybody is supposed to do all sorts of test to identify the bacteria. The first thing I did was smear my bacteria on a liquid medium. I then proceeded to incubate the medium for 24-48 hours. 1. GRAM STAIN The next step I took in finding my unknown bacteria was to gram stain it. This is used to differentiate
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